[1]李锐莉,许俐娜,袁曼,等.八核铜络合物对SGC7901细胞生长的影响[J].郑州大学学报(医学版),2012,(02):197.
 LI Ruili,XU Lina,YUAN Man,et al.Effect of eight nuclear copper complexes on growth of SGC7901 cell[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(02):197.
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八核铜络合物对SGC7901细胞生长的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年02期
页码:
197
栏目:
论著
出版日期:
2012-03-20

文章信息/Info

Title:
Effect of eight nuclear copper complexes on growth of SGC7901 cell
作者:
李锐莉许俐娜袁曼崔丙林徐霞#
郑州大学药学院药物分析教研室 郑州 450001
Author(s):
LI RuiliXU LinaYUAN Man CUI BinglinXU Xia
Department of Pharmaceutical Analysis,College of Pharmaceutical Sciences,Zhengzhou University,Zhengzhou 450001
关键词:
八核铜络合物胃癌SGC7901细胞Caspase3蛋白
Keywords:
eight nuclear copper complexesgastric neoplasmSGC7901 cellCaspase3 protein
分类号:
R917
摘要:
目的:研究八核铜络合物(NCu)对人胃癌SGC7901细胞生长的影响,探讨NCu抗肿瘤活性的相关机制。方法:体外常规培养人SGC7901细胞,用不同质量浓度的NCu(3.125、6.250、12.500、25.000、50.000、100.000 mg/L)分别处理24、48和72 h后,MTT法检测SGC7901细胞增殖抑制率,计算IC50。分别用12.5、25.0和50.0 mg/L NCu处理SGC7901细胞,倒置显微镜和扫描电镜下观察细胞形态的改变,流式细胞仪测定SGC7901细胞周期和凋亡,Westernblot法检测Caspase3蛋白的表达。结果:NCu作用24、48和72 h对SGC7901细胞的IC50分别为73.45、33.71和22.78 mg/L。随着NCu质量浓度的增加和作用时间的延长,扫描电镜下可见细胞微绒毛逐渐消失,胞质收缩,细胞呈球形,早期凋亡细胞形成膜包裹的凋亡小体凸出于细胞表面。NCu可剂量依赖性和时间依赖性地抑制SGC7901细胞的增殖,将其阻滞在G0/G1期(F组间=3 586.750,F时间= 418.133,F交互=224.383,P均<0.001)。NCu可剂量依赖性地促使SGC7901细胞发生凋亡(F=66.932,P<0.001),增强Caspase3蛋白的表达(F=55.133,P<0.001)。结论:NCu可通过诱导Caspase3蛋白的表达,参与SGC7901凋亡的调节,抑制细胞增殖。
Abstract:
Aim:To explore the effect of eight nuclear copper complexes(NCu) on the growth of SGC7901 cell.Methods:SGC7901 cells were treated with different concentrations(3.125,6.250,12.500,25.000,50.000,and 100.000 mg/L) of NCu for 24,48,and 72 h, the cell proliferation was detected by MTT assay,and IC50 was calculated.SGC7901 cells were treated with different concentrations(12.5 mg/L,25.0 mg/L,50.0 mg/L) of NCu for 24,48, and 72 h,cell cycle and apoptosis rate were detected using flow cytometry,and the expression of Caspase3 protein was detected using Westernblot assay.Results:IC50 of NCu to SGC7901 cells was 73.45,33.71 and 22.78 mg/L at 24,48 and 72 h. In time and concentrationdependent manner,NCu could inhibit SGC7901 proliferation, arrested it at G0/G1 phase(Fconcentration=3 586.750,Ftime= 418.133,P<0.001),increased the expression of Caspase3 protein(F=55.133,P<0.001), and promoted apoptosis(F=66.932,P<0.001).Conclusion: NCu could restrain the cell proliferation by regulating the apoptosis of SGC7901 cells through inducing the expression of Caspase3 partly.

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备注/Memo

备注/Memo:
#通讯作者,女,1965年6月生,博士,教授,研究方向:药物色谱分析,Email:xuxia@zzu.edu.cn
更新日期/Last Update: 1900-01-01