[1]杨铁军),何朝宏)#,曹新广),等.灯盏细辛对大鼠肾冷缺血再灌注损伤的保护作用[J].郑州大学学报(医学版),2012,(02):225.
 YANG Tiejun),HE Chaohong),CAO Xinguang),et al.Protective effects of eirgeron breviscapus(vant) handmazz on renal cold ischemia and reperfusion injury in rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(02):225.
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灯盏细辛对大鼠肾冷缺血再灌注损伤的保护作用
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年02期
页码:
225
栏目:
论著
出版日期:
2012-03-20

文章信息/Info

Title:
Protective effects of eirgeron breviscapus(vant) handmazz on renal cold ischemia and reperfusion injury in rats
作者:
杨铁军1)何朝宏1)#曹新广1)任君凯1)陈小兵1)王跃2)
1)河南省肿瘤医院;郑州大学附属肿瘤医院泌尿外科 郑州450008 2)郑州大学第一附属医院器官移植中心 郑州 450052 
Author(s):
YANG Tiejun1)HE Chaohong1)CAO Xinguang1) REN Junkai1)CHEN Xiaobing1)WANG Yue2)
1)Department of Urology, Henan Tumor Hospital;the Affiliated Tumor Hospital,Zhengzhou University,Zhengzhou 450008 2)Department of Transplantation,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
大鼠缺血再灌注损伤灯盏细辛
Keywords:
rat kidneyischemia and reperfusion injuryeirgeron breviscapus(vant) handmazz
分类号:
R692.6
摘要:
目的:探讨中药灯盏细辛对大鼠肾冷缺血再灌注损伤(IRI)的保护作用及机制。方法:采用随机数字表将36只SD大鼠分为假手术组、模型对照组和实验组3组(n=12);实验组术前15 min尾静脉注射灯盏细辛注射液1.2 mL/100 g,其他2组给予生理盐水。假手术组切除右肾并游离左肾蒂;模型对照组和实验组制作冷IRI模型。3组大鼠均在术后24 h再次手术切除左肾,采用透射电镜观察超微结构的变化,并制作肾脏组织匀浆,检测肾组织匀浆中超氧化物歧化酶(SOD)活性与丙二醛(MDA)含量。手术前后取血标本检测血浆中尿素氮(BUN)、肌酐(Cr)以评估肾脏功能。结果:①假手术组肾小球及肾小管及细胞器结构正常;模型对照组肾小管细胞呈现典型IRI,线粒体肿胀明显,部分线粒体膜撕裂,微绒毛脱落,胞质内空泡变性,部分细胞核可见凋亡迹象;实验组病变较模型对照组明显减轻。②3组肾组织匀浆SOD活及MDA含量差异有统计学意义(F分别为44.534和145.535,P<0.001)。③IRI后模型对照组、实验组血浆BUN、Cr均高于术前,且模型对照组高于实验组(BUN:F组间=88.647,F时间=188.620,F交互=88.331,P<0.001;Cr:F组间=200.470,F时间=555.184,F交互=375.640,P<0.001)。结论:灯盏细辛注射液对大鼠肾脏冷IRI具有保护作用。
Abstract:
Aim:To explore the effect and the mechanism of eirgeron breviscapus (vant) handmazz on cold ischemia and reperfusion injury in rats.Methods:Thirty six SD rats were randomly assigned to three groups:sham operation(A),model control (B), and experimental (C)group. In group C,eirgeron breviscapus (vant) handmazz was injected (1.2 mL/100 g) at 15 min before operation,meanwhile,normal saline was given in group A and B.Group A was only removed the right kidney. Cold ischemia and reperfusion was performed in group B and C. Left kidneys of all rats in three groups were removed.Structure of the left kidney was evaluated by electronic microscopy. Superoxide dismutase(SOD) activity and malondialdehyde(MDA) content in the renal tissues was examined, and the renal function was also assessed by determining the levels of blood urea nitrogen(BUN) and serum creatinine(CR) before and 24 h after operation.Results:Normal ultrastructure was observed by electron microscopy in group A. Swelling and vacuolation of mitochondrion with rare microvillus and cytoplasmic vacuolation appeared in group B, and apoptosis appeared in some nucleuses. The degree of tubular epithelium injury in the group C was milder than that in group B. Activity of SOD and content of MDA in renal tissues of the 3 groups had significant difference (F=44.534,145.535,P<0.001). BUN and CR in renal tissues of the 3 groups had significant difference BUN:Fgroup=88.647,Ftime=188.620,Finteraction=88.331,P<0.001;Cr:Fgroup=200.470,Ftime=555.184,Finteraction=375.640,P<0.001).Conclusion:Eirgeron breviscapus (vant) handmazz has protective effects on renal cold ischemia and reperfusion injury.

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备注/Memo

备注/Memo:
#通讯作者,男,1962年10月生,博士,主任医师,研究方向:泌尿系肿瘤、肾移植,Email:15981828716@163.com
更新日期/Last Update: 1900-01-01