[1]徐莉娟),代丽萍),王凯娟),等.多种肿瘤相关抗原抗体在乳癌早期诊断中的应用[J].郑州大学学报(医学版),2012,(02):241.
 XU Lijuan),DAI Liping),WANG Kaijuan),et al.Application of antibodies against a panel of multiple tumorassociated antigens in early diagnosis of breast cancer[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(02):241.
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多种肿瘤相关抗原抗体在乳癌早期诊断中的应用
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年02期
页码:
241
栏目:
论著
出版日期:
2012-03-20

文章信息/Info

Title:
Application of antibodies against a panel of multiple tumorassociated antigens in early diagnosis of breast cancer
作者:
徐莉娟12)代丽萍1)王凯娟1)张建营1)王鹏1)#
1)郑州大学公共卫生学院流行病学教研室 郑州 450001 2)郑州大学第一附属医院检验科 郑州 450052
Author(s):
XU Lijuan12)DAI Liping1)WANG Kaijuan1)ZHANG Jianying1)WANG Peng1)
1)Department of Epidemiology,College of Public Health,Zhengzhou University,Zhengzhou 450001 2)Clinical Laboratory,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
乳癌肿瘤相关抗原自身抗体早期诊断
Keywords:
breast cancertumorassociated antigenautoantibodyearly diagnosis
分类号:
R737.9
摘要:
目的:评价多种肿瘤相关抗原 (TAA) 抗体的联合检测在乳癌早期诊断中的价值,探索一种真实可靠的乳癌早期诊断方法。方法:应用酶联免疫吸附试验对115例乳癌患者和153例正常对照血清中的14种TAA抗体进行检测,应用流行病学方法对检测结果进行综合评价。结果:单独应用14种TAA中的任何一种进行检测时,灵敏度普遍偏低(最高低于33%);对14种TAA进行不同的组合,逐渐增加抗原数目进行检测,随着检测抗体的增多,诊断的灵敏度随之增加,抗原数目增加到8种(Cyclin E,Koc,Imp1,cIAP,p62,p16,Cyclin B1和Calnuc)时,灵敏度达到最高;联合8种TAA检测的灵敏度提高到72.2%,特异度达到91.2%,阳性、阴性似然比分别为8.490、0.304,阳性、阴性预测值分别为86.5%、81.4%,Kappa值0.650。结论:该诊断实验结果与真实值中度一致,8种TAA联合检测乳癌的诊断价值较高。
Abstract:
Aim:To evaluate the diagnostic value of antibodies against multiple tumorassociated antigens (TAAs) in the early diagnosis of breast cancer and to develop a valid and reliable diagnostic method for breast cancer.Methods:Enzymelinked immunosorbent assay (ELISA) was used to detect antibodies against fourteen TAAs in 115 sera samples from patients with breast cancer,and also in 153 sera samples from normal individuals.Epidemiological methods were used to evaluate the testing results for each antiTAA antibody.Results:The sensitivities were low(the highest below 33% )when a separate TAA was used;with the successive addition of TAAs,there was a stepwise increase of sensitivity.The sensitivity did not increase until when there were eight TAAs (Cyclin E,Koc,Imp1,cIAP,p62,p16,Cyclin B1 and Calnuc).The positive antibody reaction of the eight TAAs reached a sensitivity of 72.2% and a specificity of 91.2%.Positive likelihood ratio and negative likelihood ratio were 8.490 and 0.304.Positive and negative predictive values were 86.5% and 81.4%. Kappa value was 0.650.Conclusion:The observed value of this assay had midrange coincidence with actual value;there was a high diagnosis value with 8 TAAs combined detection of breast cancer.

参考文献/References:

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备注/Memo

备注/Memo:
#通讯作者,女,1981年3月生,硕士,讲师,研究方向:肿瘤流行病学,Email:wangpeng@zzu.edu.cn
更新日期/Last Update: 1900-01-01