[1]刘月强,赵素贞,孟凡民,等.单次低剂量丙泊酚联合肢体缺血后处理对大鼠脑缺血再灌注损伤的影响[J].郑州大学学报(医学版),2012,(02):259.
　LIU Yueqiang,ZHAO Suzhen,MENG Fanmin,et al.Effects of single low dose propofol with limb ischemia postconditioning on cerebral ischemia/reperfusion injury in rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(02):259.
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单次低剂量丙泊酚联合肢体缺血后处理对大鼠脑缺血再灌注损伤的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年02期
页码:
259
栏目:
应用研究
出版日期:
2012-03-20

文章信息/Info

Title:
Effects of single low dose propofol with limb ischemia postconditioning on cerebral ischemia/reperfusion injury in rats
作者:
刘月强; 赵素贞; 孟凡民; 崔明珠#
河南省人民医院麻醉科 郑州 450003
Author(s):
LIU Yueqiang;  ZHAO Suzhen;  MENG Fanmin;  CUI Mingzhu
Department of Anesthesiology，Henan Provincial People’s Hospital，Zhengzhou 450003
关键词:
丙泊酚; 肢体缺血后处理; 脑缺血再灌注; 氧化应激; 炎症反应; 大鼠
Keywords:
propofol; limb ischemia postconditioning;  cerebral ischemia/reperfusion;  oxidative stress;  inflammation reaction; rat
分类号:
R743
摘要:
目的：探讨单次低剂量丙泊酚联合肢体缺血后处理(LIPC)对大鼠脑缺血再灌注损伤的影响。方法：100只雄性SD大鼠分为5组：假手术组、模型组、丙泊酚组、LIPC组和联合治疗组（丙泊酚＋LIPC）,每组20只。采用线栓闭塞大脑中动脉法建立局灶性脑缺血再灌注模型。测定神经功能缺陷评分，脑梗死面积，MDA含量，SOD、GSHPx活性以及IL6、TNFα水平。 结果：5组大鼠以上指标相比，差异均有统计学意义（P<0.001）；低剂量丙泊酚和LIPC均能改善脑缺血再灌注大鼠神经功能缺损症状，减少脑梗死面积，降低IL6水平、TNFα水平及MDA含量，抑制SOD、GSHPx活性的下降，而丙泊酚与LIPC联合应用效果更为明显（P<0.05）。结论：低剂量丙泊酚联合LIPC可通过抑制氧化应激和炎症反应减轻脑缺血再灌注损伤，两者合用具有协同效果。
Abstract:
Aim:To investigate the effects of single low dose propofol with limb ischemia postconditioning(LIPC) on cerebral ischemia/reperfusion injury in rats.Methods:A total of 100 male SD rats were randomly allocated into five groups:sham group,model group, propofol group,LIPC group and combined treatment group(propofol plus LIPC),20 in each group.The focal cerebral ischemia/reperfusion rat model was set up by using the middle cerebral artery occlusion method. The neurological deficit score, infarct size, malondialdehyde (MDA) content,superoxide dismutase(SOD) and glutathione peroxidase (GSHPx) activities, interleukin6(IL6) and tumor necrosis factorα(TNFα) levels were detected.Results: There were significant differences in the above parameters among the 5 groups(P<0.001);low dose propofol and LIPC both could improve neurological deficit symptoms,reduce infarct size, decrease IL6,TNFα level and MDA content, inhibit the decrease of SOD and GSHPx activities in cerebral ischemia/reperfusion rats, while the effect of propofol with LIPC combinated treatment was more obvious (P<0.05).Conclusion: Low dose propofol with LIPC is able to reduce cerebral ischemia/reperfusion injury by inhibiting oxidative stress and inflammation reaction,and combined treatment displays synergistic effect.

参考文献/References:

［1］Selim M. Perioperative stroke［J］. New Eng J Med,2007,356（7）:706
［2］Fairbanks SL, Brambrink AM. Preconditioning and postconditioning for neuroprotection: The most recent evidence ［J］. Best Pract Res Clin Anaesthesiol,2010,24（4）:521
［3］李喆, 覃晓. 丙泊酚在缺血/再灌注损伤中的保护机制［J］. 医学综述, 2011,17(3):444
［4］林志峰,蔡婉玲.丙泊酚对大鼠脑缺血再灌注损伤后的保护作用及机制研究［J］.临床合理用药,2011,4(4C):16
［5］Fudickar A,Bein B,Tonner PH. Propofol infusion syndrome in anaesthesia and intensive care medicine［J］. Curr Opin Anaesthesiol,2006,19(4): 404
［6］杨定东,戴泽平,朱美芳,等.雷米芬太尼和丙泊酚对老年大鼠认知功能的影响［J］.临床麻醉学杂志,2009,25(1):48
［7］蒋燕,周泽军,高进,等.反复注射异丙酚对新生大鼠认知功能的影响［J］.中华麻醉学杂志,2008,28(11):1007
［8］冯静,柴东燕,秦玉花,等.黄杞总黄酮对脑缺血再灌注大鼠的脑保护作用［J］.郑州大学学报:医学版,2011,46(6):910
［9］Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats ［J］. Stroke,1989,20(1):84
［10］郭建荣,丁节清,胡馨.丙泊酚对大鼠脑缺血再灌注损伤后海马线粒体ATP含量和ATP酶活性的影响［J］.临床麻醉学杂志,2005,21(10):716
［11］徐磊,鲍红光,徐建国.丙泊酚对大鼠缺血再灌注脑TNFα、IL10、NFκB的影响［J］.临床麻醉学杂志,2009,25(5):424
［12］Murphy PG, Myers DS, Davies MJ, et al. The antioxidant potential of propofol (2, 6diisopropylphenol) ［J］. Br J Anaesth,1992,68(6):613
［13］Chen HI, Hsieh NK, Kao SJ, et al. Protective effects of propofol on acute lung injury induced by oleic acid in conscious rats ［J］. Crit Care Med,2008,36(4):1214
［14］赵瑾, 李杨, 黄磊, 等. 肢体缺血后适应对小鼠脑缺血再灌注损伤的影响及机制［J］. 中国病理生理杂志,2010,26(6):1096
［15］吴莉萍. 线粒体ATP敏感性钾通道介导肢体缺血后处理对抗大鼠局灶性脑缺血再灌注损伤的神经保护作用［D］.杭州：浙江大学,2006.

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备注/Memo

备注/Memo:
#通讯作者，男，1978年6月生，硕士，主治医师，研究方向：心脏手术麻醉与器官保护，Email:pearlcui@163.com
更新日期/Last Update: 1900-01-01