[1]刘月强,赵素贞,孟凡民,等.单次低剂量丙泊酚联合肢体缺血后处理对大鼠脑缺血再灌注损伤的影响[J].郑州大学学报(医学版),2012,(02):259.
 LIU Yueqiang,ZHAO Suzhen,MENG Fanmin,et al.Effects of single low dose propofol with limb ischemia postconditioning on cerebral ischemia/reperfusion injury in rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(02):259.
点击复制

单次低剂量丙泊酚联合肢体缺血后处理对大鼠脑缺血再灌注损伤的影响
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年02期
页码:
259
栏目:
应用研究
出版日期:
2012-03-20

文章信息/Info

Title:
Effects of single low dose propofol with limb ischemia postconditioning on cerebral ischemia/reperfusion injury in rats
作者:
刘月强赵素贞孟凡民崔明珠#
河南省人民医院麻醉科 郑州 450003
Author(s):
LIU Yueqiang ZHAO Suzhen MENG Fanmin CUI Mingzhu
Department of Anesthesiology,Henan Provincial People’s Hospital,Zhengzhou 450003
关键词:
丙泊酚肢体缺血后处理脑缺血再灌注氧化应激炎症反应大鼠
Keywords:
propofollimb ischemia postconditioning cerebral ischemia/reperfusion oxidative stress inflammation reactionrat
分类号:
R743
摘要:
目的:探讨单次低剂量丙泊酚联合肢体缺血后处理(LIPC)对大鼠脑缺血再灌注损伤的影响。方法:100只雄性SD大鼠分为5组:假手术组、模型组、丙泊酚组、LIPC组和联合治疗组(丙泊酚+LIPC),每组20只。采用线栓闭塞大脑中动脉法建立局灶性脑缺血再灌注模型。测定神经功能缺陷评分,脑梗死面积,MDA含量,SOD、GSHPx活性以及IL6、TNFα水平。 结果:5组大鼠以上指标相比,差异均有统计学意义(P<0.001);低剂量丙泊酚和LIPC均能改善脑缺血再灌注大鼠神经功能缺损症状,减少脑梗死面积,降低IL6水平、TNFα水平及MDA含量,抑制SOD、GSHPx活性的下降,而丙泊酚与LIPC联合应用效果更为明显(P<0.05)。结论:低剂量丙泊酚联合LIPC可通过抑制氧化应激和炎症反应减轻脑缺血再灌注损伤,两者合用具有协同效果。
Abstract:
Aim:To investigate the effects of single low dose propofol with limb ischemia postconditioning(LIPC) on cerebral ischemia/reperfusion injury in rats.Methods:A total of 100 male SD rats were randomly allocated into five groups:sham group,model group, propofol group,LIPC group and combined treatment group(propofol plus LIPC),20 in each group.The focal cerebral ischemia/reperfusion rat model was set up by using the middle cerebral artery occlusion method. The neurological deficit score, infarct size, malondialdehyde (MDA) content,superoxide dismutase(SOD) and glutathione peroxidase (GSHPx) activities, interleukin6(IL6) and tumor necrosis factorα(TNFα) levels were detected.Results: There were significant differences in the above parameters among the 5 groups(P<0.001);low dose propofol and LIPC both could improve neurological deficit symptoms,reduce infarct size, decrease IL6,TNFα level and MDA content, inhibit the decrease of SOD and GSHPx activities in cerebral ischemia/reperfusion rats, while the effect of propofol with LIPC combinated treatment was more obvious (P<0.05).Conclusion: Low dose propofol with LIPC is able to reduce cerebral ischemia/reperfusion injury by inhibiting oxidative stress and inflammation reaction,and combined treatment displays synergistic effect.

参考文献/References:

[1]Selim M. Perioperative stroke[J]. New Eng J Med,2007,356(7):706
[2]Fairbanks SL, Brambrink AM. Preconditioning and postconditioning for neuroprotection: The most recent evidence [J]. Best Pract Res Clin Anaesthesiol,2010,24(4):521
[3]李喆, 覃晓. 丙泊酚在缺血/再灌注损伤中的保护机制[J]. 医学综述, 2011,17(3):444
[4]林志峰,蔡婉玲.丙泊酚对大鼠脑缺血再灌注损伤后的保护作用及机制研究[J].临床合理用药,2011,4(4C):16
[5]Fudickar A,Bein B,Tonner PH. Propofol infusion syndrome in anaesthesia and intensive care medicine[J]. Curr Opin Anaesthesiol,2006,19(4): 404
[6]杨定东,戴泽平,朱美芳,等.雷米芬太尼和丙泊酚对老年大鼠认知功能的影响[J].临床麻醉学杂志,2009,25(1):48
[7]蒋燕,周泽军,高进,等.反复注射异丙酚对新生大鼠认知功能的影响[J].中华麻醉学杂志,2008,28(11):1007
[8]冯静,柴东燕,秦玉花,等.黄杞总黄酮对脑缺血再灌注大鼠的脑保护作用[J].郑州大学学报:医学版,2011,46(6):910
[9]Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats [J]. Stroke,1989,20(1):84
[10]郭建荣,丁节清,胡馨.丙泊酚对大鼠脑缺血再灌注损伤后海马线粒体ATP含量和ATP酶活性的影响[J].临床麻醉学杂志,2005,21(10):716
[11]徐磊,鲍红光,徐建国.丙泊酚对大鼠缺血再灌注脑TNFα、IL10、NFκB的影响[J].临床麻醉学杂志,2009,25(5):424
[12]Murphy PG, Myers DS, Davies MJ, et al. The antioxidant potential of propofol (2, 6diisopropylphenol) [J]. Br J Anaesth,1992,68(6):613
[13]Chen HI, Hsieh NK, Kao SJ, et al. Protective effects of propofol on acute lung injury induced by oleic acid in conscious rats [J]. Crit Care Med,2008,36(4):1214
[14]赵瑾, 李杨, 黄磊, 等. 肢体缺血后适应对小鼠脑缺血再灌注损伤的影响及机制[J]. 中国病理生理杂志,2010,26(6):1096
[15]吴莉萍. 线粒体ATP敏感性钾通道介导肢体缺血后处理对抗大鼠局灶性脑缺血再灌注损伤的神经保护作用[D].杭州:浙江大学,2006.

相似文献/References:

[1]赵璞,张卫.舒芬太尼复合丙泊酚在无痛膀胱镜检查中的应用[J].郑州大学学报(医学版),2010,(06):1017.
[2]吕慧敏,张卫.七氟醚复合笑气吸入联合气管内表面麻醉用于小儿气道异物取出术25例体会[J].郑州大学学报(医学版),2010,(06):1027.
[3]宋晓荣)△,千智斌),姬明丽).尼可刹米对丙泊酚抑制新生大鼠延髓脑片呼吸放电的拮抗作用*[J].郑州大学学报(医学版),2011,(03):388.
 SONG Xiaorong),QIAN Zhibin),JI Mingli).Antagonistic effects of nikethamide on propofol depressing respiratory discharge of medullary slices in neonatal rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(02):388.
[4]王红玉,李治松,杜英英,等.不同品牌丙泊酚靶控输注对肌袢代瓣术中患者意识消失的影响[J].郑州大学学报(医学版),2012,(03):424.
[5]巴玉峰),冯惠民),李长生),等.丙泊酚预处理对单肺机械通气大鼠肺组织血红素氧合酶1表达的影响[J].郑州大学学报(医学版),2012,(04):500.
 BA Yufeng,FENG Huimin,LI Changsheng,et al.Effect of preconditioning with propofol on expression of HO1 protein in rat lung tissue during one lung ventilation[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(02):500.
[6]冯惠民,李廷坤,吕帅国,等.丙泊酚麻醉对肝癌射频消融术患者血清白介素和HSP70水平的影响[J].郑州大学学报(医学版),2012,(04):542.
 FENG Huimin,LI Tingkun,LV Shuaiguo,et al.Effects of propofol anesthesia on serum interleukins and HSP70 levels in patients with liver cancer after radiofrequency ablation[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(02):542.
[7]高巍,宋平义,景桂霞,等.丙泊酚对HepG2肝母细胞瘤细胞侵袭能力的影响*[J].郑州大学学报(医学版),2015,(03):318.
 GAO Wei,SONG Pingyi,JING Guixia,et al.Effects of propofol on the invasion ability of hepatoblastoma HepG2 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(02):318.

备注/Memo

备注/Memo:
#通讯作者,男,1978年6月生,硕士,主治医师,研究方向:心脏手术麻醉与器官保护,Email:pearlcui@163.com
更新日期/Last Update: 1900-01-01