[1]刘宗文),冯付明),王亚莉),等.食管鳞状细胞癌TE13细胞线粒体DNA及细胞凋亡检测*[J].郑州大学学报(医学版),2012,(03):281.
 LIU Zongwen),FENG Fuming),WANG Yali),et al.Detection of mitochondrial DNA and apoptosis in esophageal squamous cell carcinoma TE13 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(03):281.
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食管鳞状细胞癌TE13细胞线粒体DNA及细胞凋亡检测*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年03期
页码:
281
栏目:
食管癌研究
出版日期:
2012-05-20

文章信息/Info

Title:
Detection of mitochondrial DNA and apoptosis in esophageal squamous cell carcinoma TE13 cells
作者:
刘宗文1)冯付明2)王亚莉3)杨建萍2)庞霞2)王峰4)郭凤玲5)郑国香6)闫俊宇7)张功员8)陈奎生2)#
1)郑州大学第二附属医院肿瘤科 郑州 450014 2)郑州大学第一附属医院病理科;河南省肿瘤病理重点实验室 郑州 450052 3)新乡医学院生理学与神经生物学教研室 新乡 453100 4)郑州大学第一附属医院肿瘤科 郑州 450052 5)河南省中医院内科 郑州 450002 6)郑州幼儿师范学校 郑州 450000 7)郑州大学外语学院 郑州 450001 8)郑州大学基础医学院组织学与胚胎学教研室 郑州 450001
Author(s):
LIU Zongwen1)FENG Fuming2)WANG Yali3)YANG Jianping2)PANG Xia2)WANG Feng4)GUO Fengling5)ZHENG Guoxiang6)YAN Junyu7)ZHANG Gongyuan8) CHEN Kuisheng2)
1)Department of Oncology,the Second Affiliated Hospital,Zhengzhou 450014 2)Department of Pathology, the First Affiliated Hospital,Zhengzhou 450052 3)Department of Physiology and Neurobiology,Xinxiang 453100 4)Department of Oncology,the First Affiliated Hospital,Zhengzhou 450052 5)Department of Clinical Medicine,Zhengzhou 450002 6)Zhengzhou Kindergarten Teachers’ School,Zhengzhou 450000 7)School of Foreign Languages,Zhengzhou 450001 8)Department of Histology and Embryology,Zhengzhou 450001
关键词:
食管肿瘤鳞状细胞癌TE13细胞线粒体DNA凋亡
Keywords:
esophageal neoplasmsquamous cell carcinomaTE13 cellmitochondrial DNAapoptosis
分类号:
R735.1
摘要:
目的:检测人食管鳞状细胞癌TE13细胞中线粒体DNA(mtDNA)与细胞凋亡的发生情况及可能的关系。方法:TE13细胞分为2组:溴化乙啶(EB)处理组在细胞培养液中加入50 mg/L EB,未处理组常规培养;在培养第4、8和12天分别利用半定量RTPCR检测2组细胞mtDNA的表达,利用流式细胞术检测细胞的凋亡情况。结果:EB处理组的第4天和第8天mtDNA的表达量分别为(0.467±0.031)和(0.197±0.015),到第12天 mtDNA已完全丢失,未处理组分别为(0.660±0.046)、(0.673±0.031)和(0.653±0.021),差异有统计学意义(F组间=1 133.878,F时间=109.058,F交互=104.597;P<0.001)。流式细胞术检测结果显示,EB处理组凋亡细胞百分比高于未处理组(F组间=2 773.035,F时间=407.652,F交互=406.641;P<0.001)。结论:抑制TE13细胞中mtDNA的复制可能会促进细胞凋亡。
Abstract:
Aim:To detect the mitochondrial DNA(mtDNA) and apoptosis in esophageal squamous cell carcinoma(ESCC) TE13 cells.Methods:TE13 cells were allocated into 2 groups:EB treatment group and control group.RTPCR was used to detect the expression of mtDNA,and apoptosis in the cells was detected by flow cytometry at days 4,8 and 12.Results:The result of mtDNA expression in EB treatment group gradually decreased from (0.467±0.031),(0.197±0.015) to 0 at days 4,8 and 12,control group was (0.660±0.046),(0.673±0.031)and(0.653±0.021),and there were significant differences between the two groups(Fgroup=1 133.878,Ftime=109.058,Finteraction=104.597;P<0.001). The results of flow cytometry revealed that cell apoptosis rates in EB treatment group were significantly higher than those of control group at days 4,8 and 12 (Fgroup=2 773.035,Ftime=407.652,Finteraction=406.641;P<0.001).Conclusion:The inhibition of mtDNA duplication may prompt the apoptosis of TE13 cells.

参考文献/References:

[1]Lindolm D, Eriksson O, Korhonen L. Mitochondrial proteins in neuronal degeneration[J]. Biochem Biophys Res Commun, 2004,321(4):753
[2]Dasgupta S, Koch R, Westra WH, et al. Mitochondrial DNA mutation in normal margins and tumors of recurrent head and neck squamous cell carcinoma patients[J].Cancer Prev Res (Phila),2010,3(9):1205
[3]Davis CD, Emenaker NJ, Milner JA. Cellular proliferation, apoptosis and angiogenesis: molecular targets for nutritional preemption of cancer[J]. Semin Oncol, 2010, 37 (3):243
[4]Krakstad C, Chekenya M. Survival signalling and apoptosis resistance in glioblastomas: opportunities for targeted therapeutics[J]. Mol Cancer, 2010, 9:135
[5]李超,伏圣博,刘华玲,等.细胞凋亡研究进展[J].世界科技研究与发展, 2007, 29(3): 45
[6]Norberg E, Orrenius S, Zhivotovsky B. Mitochondrial regulation of cell death: processing of apoptosisinducing factor (AIF) [J]. Biochem Biophys Res Commun, 2010, 396(1):95
[7]戴纪刚,闵家新,张国强,等.线粒体DNA缺失细胞系的建立[J].第三军医大学学报,2005,27(6):570
[8]张国桥,凌贤龙,陈正堂.人线粒体DNA缺失肝癌细胞株建立[J].细胞生物学杂志,2007,29(1):127
[9]Eaton JS, Lin ZP, Sartorelli AC, et al. Ataxiatelangiectasia mutated kinase regulates ribonucleotide reductase and mitochondrial homeostasis[J]. J Clin Invest, 2007, 117(9): 2723
[10]Carew JS,Huang P.Mitochondrial defects in cancer[J].Mol Cancer,2002,1:9
[11]DaliYoucef N, Mataki C, Coste A, et al. Adipose tissuespecific inactivation of the retinoblastoma protein protects against diabesity because of increased energy expenditure[J]. Proc Natl Acad Sci USA,2007,104(25):10703
[12]Pelicano H, Xu RH, Du M, et al. Mitochondrial respiration defects in cancer cells cause activation of Akt survival pathway through a redoxmediated mechanism[J]. J Cell Biol,2006,175(6):913

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备注/Memo

备注/Memo:
*河南省科技攻关计划基金资助项目72102310104;河南省教育厅自然科学研究计划基金资助项目2008A310016 #通讯作者,男,1964年8月生,博士,教授,研究方向:肿瘤病理学,Email:chenksh2002@yahoo.com.cn
更新日期/Last Update: 2012-05-31