[1]高峰),李立),张俊杰),等.重症肌无力患者胸腺组织中共刺激分子B7H1的表达*[J].郑州大学学报(医学版),2012,(03):287.
 GAO Feng),LI Li),ZHANG Junjie),et al.Expression of B7H1 in thymus of patients with myasthenia gravis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(03):287.
点击复制

重症肌无力患者胸腺组织中共刺激分子B7H1的表达*
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年03期
页码:
287
栏目:
专题研究
出版日期:
2012-05-20

文章信息/Info

Title:
Expression of B7H1 in thymus of patients with myasthenia gravis
作者:
高峰1)李立2)张俊杰3)方华1)刘素娜1)杜英4)关兵峰56)张清勇56)#
1)郑州大学医药科学研究院免疫学研究室神经免疫实验室 郑州 450052 2)河南省医学科学院 郑州 450003 3)周口市中医院胸外科 周口466000 4)郑州大学基础医学院微生物学与免疫学教研室 郑州 450001 5)郑州大学第二附属医院胸外科 郑州 450014 6)郑州大学生物医学工程研究所 郑州 450014
Author(s):
GAO Feng1)LI Li2)ZHANG Junjie3)FANG Hua1)LIU Suna1)DU Ying4)GUAN Bingfeng56)ZHANG Qingyong56)
1)Lab of Neuroimmunology,Department of Immunology Research,Academy of Medical and Pharmaceutical Sciences,Zhengzhou 450052 2)Henan Academy of Medical Sciences, Zhengzhou 450003 3)Department of Thoracic Surgery,Zhoukou 466000 4)Department of Microbiology and Immunology,Zhengzhou 450001 5)Department of Thoracic Surgery,Zhengzhou 450014 6)Institute of Biological Medicine,Zhengzhou 450014
关键词:
B7H1胸腺组织重症肌无力
Keywords:
B7H1thymusmyasthenia gravis
分类号:
R593.2
摘要:
目的:检测重症肌无力(MG)患者胸腺组织中共刺激分子B7H1的表达。方法:以正常胸腺组织为对照,采用RTPCR法检测胸腺增生(14例)、胸腺萎缩(5例)和胸腺瘤(6例)组织中B7H1 mRNA的表达,流式细胞仪分析胸腺组织中B7H1蛋白的表达情况,免疫组化染色方法观察胸腺组织中B7H1蛋白的分布。结果:不同类型胸腺组织中BHH1 mRNA和蛋白表达差异有统计学意义(FmRNA=5.152,P=0.005;F蛋白=116.049,P<0.001)。免疫组化染色结果显示,B7H1蛋白阳性信号主要定位于胸腺组织的皮髓质交界处和髓质胸腺细胞膜上,胸腺增生组织中B7H1蛋白阳性胸腺细胞数量较多,分布密集,信号强度高。结论:B7H1 mRNA和蛋白的高表达可能与MG胸腺组织异常增生有关。
Abstract:
Aim:To detect the expression of costimulatory molecule B7H1 in thymus tissue of myasthenia gravis (MG) patients.Methods:With normal thymus as control, expression level of B7H1 mRNA in the thymus tissue from 25 patients with MG was detected by RTPCR, while tissue distribution and expression level of B7H1 proteins in different pathological types of MG were detected by immunohistochemical staining and flow cytometry, respectively.Results:The expression of B7H1 mRNA and protein were significantly different among the 4 groups (FmRNA=5.152,P=0.005;Fprotein=116.049,P<0.001).Immunohistochemical staining showed that the B7H1 protein was mainly located in the membrane of thymus cell in the thymic medulla and the junction between thymic cortex and medulla.Conclusion:The high expression of B7H1 mRNA and protein may interfere with the thymic dysplasia in MG patients.

参考文献/References:

[1]吴元波. CD4+CD25+调节性T细胞对成人眼肌型重症肌无力临床转归的影响[D]. 合肥:安徽医科大学,2011.
[2]闫晓海. 重症肌无力患者伴胸腺增生及胸腺瘤的电生理特点分析[J]. 中国实用医药,2011,6(2):114
[3]Tackenberg B, Kruth J, Bartholomaeus JE, et al. Clonal expansions of CD4+ B helper T cells in autoimmune myasthenia gravis[J]. Eur J Immunol, 2007,37(3):849
[4]Prelog M, Keller M,Geiger R, et al. Thymectomy in early childhood:significant alterations of the CD4(+)CD45RA(+)CD62L(+) T cell compartment in later life[J].Clin Immunol,2009,130(2):123
[5]韩利勋, 侯玉芹, 白伟利. 重症肌无力患者手术前后血清白介素6水平研究[J]. 中国实用神经疾病杂志,2009,12(14):38
[6]孟青, 刘书漫, 王一菱, 等. 食管鳞状细胞癌组织中B7H1蛋白和mRNA的表达[J]. 郑州大学学报: 医学版, 2009,44(1):48
[7]Sakthivel P, Ramanujam R, Wang XB,et al.Programmed death1: from gene to protein in autoimmune human myasthenia gravis[J].J Neuroimmunol,2008,193(1/2):149
[8]张憨,李柱一. 胸腺与重症肌无力[J]. 中国神经免疫学和神经病学杂志,2011,18(5):361
[9]迟小伟, 刘杰. B7CD28/CTLA4在胃癌患者中的表达及意义[J]. 山东医药,2010,50(35):33
[10]Aricha R, Feferman T, Fuchs S, et al. Ex vivo generated regulatory T cells modulate experimental autoimmune myasthenia gravis[J].J Immunol,2008,180(4):2132
[11]Keir ME,Butte MJ,Freeman GJ,et al.PD1 and its ligands in tolerance and immunity[J].Annu Rev Immunol,2008,26:677
[12]Afaneh C, Muthukumar T, Lubetzky M, et al. Urinary cell levels of mRNA for OX40, OX40L, PD1,PDL1 or PDL2 and acute rejection of human renal allografts[J].Transplantation, 2010, 90(12): 1381
[13]祖莹, 李成荣, 李德发, 等. 支气管哮喘患儿 B7家族共刺激分子及其受体表达的变化[J]. 实用儿科临床杂志,2009,24(4):271
[14]Mu CY,Huang JA, Chen Y, et al. High expression of PDL1 in lung cancer may contribute to poor prognosis and tumor cell immune escape through suppressing tumor infiltrating dendritic cells maturation[J]. Med Oncol,2011,28(3):682
[15]Geng L, Deng J, Jiang G, et al. B7H1 upregulated expression in human hepatocellular carcinoma tissue:correlation with tumor interleukin10 levels[J].Hepatogastroenterology, 2011, 58 (107/108):960
[16]Frigola X, Inman BA, Lohse CM, et al. Identification of a soluble form of B7H1 that retains immunosuppressive activity and is associated with aggressive renal cell carcinoma[J].CLin Cancer Res, 2011, 17 (7): 1915
[17] Jia R, Jiao Z, Xu X, et al. Functional significance of B7H1 expressed by human uveal melanoma cells[J].Mol Med Report,2011,4(1):163

相似文献/References:

[1]孟青)),刘书漫),王一菱),等.食管鳞状细胞癌组织中B7H1蛋白和mRNA的表达*[J].郑州大学学报(医学版),2009,(01):48.
 MENG Qing)),LIU Shuman),WANG Yiling),et al.Expression of B7H1 mRNA and protein in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):48.

备注/Memo

备注/Memo:
*国家自然科学基金资助项目30570625;河南省医学科技攻关基金资助项目201003147;河南省基础与前沿基金资助项目072300450080 #通讯作者,男,1963年11月生,主任医师,教授,研究方向:重症肌无力的临床医学与发病机制,Email:qyzhang@zzu.edu.cn
更新日期/Last Update: 2012-05-31