[1]李永丽),李立),李静孟),等.重症肌无力患者胸腺增生组织中11 000条带的蛋白质组学分析*[J].郑州大学学报(医学版),2012,(03):291.
 LI Yongli),LI Li),LI Jingmeng),et al.Proteomics analysis of 11 000 protein band of myasthenia gravis patients with thymic hyperplasia[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(03):291.
点击复制

重症肌无力患者胸腺增生组织中11 000条带的蛋白质组学分析*
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年03期
页码:
291
栏目:
专题研究
出版日期:
2012-05-20

文章信息/Info

Title:
Proteomics analysis of 11 000 protein band of myasthenia gravis patients with thymic hyperplasia
作者:
李永丽1) 李立2)李静孟3)朱利伟4)崔新征56)方华7)杨鲲鹏5)张清勇56)#高峰7)
1)河南省人民医院放射科 郑州 450003 2)河南省医学科学院 郑州 450003 3)第三军医大学附属新桥医院胸外科 重庆 4000374)漯河医学高等专科学校第三附属医院外科 漯河 462002 5)郑州大学第二附属医院胸外科 郑州450014 6)郑州大学生物医学工程研究所 郑州 450014 7)郑州大学医药科学研究院免疫学研究室神经免疫实验室 郑州 450052
Author(s):
LI Yongli1)LI Li2)LI Jingmeng3)ZHU Liwei4)CUI Xinzheng56) FANG Hua7) YANG Kunpeng5) ZHANG Qingyong56) GAO Feng7)
1)Department of Radiology,Zhengzhou 450003 2) Henan Academy of Medical Sciences, Zhengzhou 450003 3) Department of Thoracic Surgery of Xinqiao Hospital,Chongqing 400037 4) Department of Surgery,Luohe 462002 5) Department of Thoracic Surgery, Zhengzhou 450014 6)Institute of Biological Medicine,Zhengzhou 450014 7)Lab of Neuroimmunology,Zhengzhou University,Zhengzhou 450052
关键词:
重症肌无力蛋白质组学基质辅助激光解析电离飞行时间质谱双向电泳4.1R蛋白
Keywords:
myasthenia gravisproteomicsMALDITOFMStwodimensional electrophoresisprotein 4.1R
分类号:
R593.2
摘要:
目的:探讨重症肌无力(MG)患者胸腺增生组织中相对分子质量为11 000差异(异常)条带的蛋白质组成。方法:采用非还原性SDSPAGE制备型电泳对11 000条带进行筛选和富集;应用双向电泳得到蛋白质二维图谱,并对差异蛋白点进行质谱分析;应用免疫组化法比较20例MG胸腺增生组织和10例正常对照组织中4.1R蛋白的表达水平。结果:MG患者胸腺增生组织中目标条带的阳性率为72.7%(8/11),该条带由(30±6)个蛋白点组成,质谱分析显示有16个高表达差异蛋白点,其中3个蛋白得到鉴定,分别为4.1R蛋白、自整合抑制因子1和胆碱乙酰转移酶。免疫组化结果显示,与正常对照组的(0.98±0.18)相比,MG患者胸腺增生组织中4.1R蛋白表达水平为(1.55±0.46)明显增高(t=3.054,P=0.016)。结论:MG胸腺增生组织11 000条带由多个蛋白点构成,这些蛋白主要功能涉及细胞骨架的组成、细胞内信息传递和乙酰胆碱合成等。
Abstract:
Aim:To discuss the formation and expression of abnormal 11 000 protein band from the tissue of myasthenia gravis patients(MG) with thymic hyperplasia.Methods:The 11 000 protein band was screened and enriched by nonreducing SDSPAGE.The electrophoretogram was obtained from two dimensional electrophoresis,and differential proteins were analyzed by MS. The expression of protein 4.1R was compared between the experimental group(20 cases of MG) and control group(10 cases of normal control) using the method of immunohistochemistry.Results:The positive rate of 11 000 protein band was 72.7%(8/11),which was consisted of(30±6)spots.16 protein spots expressed with higher level,among which,3 were identified as protein 4.1R, barrier to autointegration factor 1 and choline acetyltransferase,respectively. Immunohistochemical staining results showed that protein 4.1R from MG patients(1.55±0.46)was higher than that of control group(0.98±0.18)(t=3.054,P=0.016).Conclusion: The 11 000 protein band is consisted of many protein spots, and main function of them is associated with the processes of cytoskeleton formation, signal transduction and acetylcholine synthesis.

参考文献/References:

[1]Aarli JA, Gilhus NE, Lisak RP, et al. Myasthenia gravis[J]. Autoimmune Dis, 2011: 697575.Epub 2012 Jan 24.
[2]Baggi F, Antozzi C, Toscani C, et al. Acetylcholine receptorinduced experimental myasthenia gravis: what have we learned from animal models after three decades?[J]. Arch Immunol Ther Exp (Warsz), 2012, 60(1): 19
[3]苏艳宾, 李倩如, 高峰, 等. 趋化因子及其受体在重症肌无力患者胸腺组织的异常表达[J]. 现代免疫学, 2011, 31 (3): 248
[4]李明月. 电视胸腔镜胸腺切除治疗重症肌无力临床效果分析[J]. 中外医学研究,2011,9(15):5
[5]刘国红, 杨继要, 呼琳, 等. 胃腺癌及正常胃黏膜组织蛋白质组双向电泳图谱分析[J]. 郑州大学学报:医学版,2009,44(4):786
[6]刘佳,姜曦,王少华,等.NV蛋白酶含量检测方法的准确度评价[J].吉林大学学报:医学版,2009,35(4):762
[7]Wang Y, Cheng Z, Lu X, et al. Echinococcus multilocularis:proteomic analysis of the protoscoleces by twodimensional electrophoresis and mass spectrometry[J]. Exp Parasitol, 2009, 123(2):162
[8]Gasteiger E, Jung E, Bairoch A. SWISSPROT: connecting biomolecular knowledge via a protein database[J]. Curr Issues Mol Biol,2001,3(3):47
[9]Huang da W, Sherman BT, Lempicki RA. Systematic and integrative analysis of large gene lists using DAVID Bioinformatics Resources[J]. Nat Protoc,2009,4(1):44
[10]李枫. 图像分析中光密度参数物理意义的正确理解和使用[J]. 解剖学杂志,2009,32(2):271
[11]Cheng C,Wu G,Yeung SC,et al.Serum protein profiles in myasthenia gravis[J].Ann Thorac Surg,2009,88(4):1118
[12]巫国勇, 庞景灼, 程超, 等. 血清蛋白质组学在重症肌无力诊断中的应用[J]. 中华医学杂志, 2009, 89(31): 2184
[13]刘萍, 丁旭峰, 章怡祎, 等. 双向电泳质谱法探索实验性重症肌无力大鼠疾病相关蛋白[J]. 中华中医药学刊, 2007, 25(12): 2486
[14]Montes de Oca R, Shoemaker CJ, Gucek M, et al. Barriertoautointegration factor proteome reveals chromatinregulatory partners[J]. PLoS One, 2009, 4(9):e7050
[15]SeguraTotten M, Wilson KL. BAF: roles in chromatin, nuclear structure and retrovirus integration[J]. Trends Cell Biol, 2004, 14(5):261
[16]Wessler I, Kirkpatrick CJ, Racké K. Nonneuronal acetylcholine, a locally acting molecule, widely distributed in biological systems: expression and function in human[J]. Pharmacol Ther, 1998, 77(1):59
[17]Huang SC, Liu ES, Chan SH, et al. Mitotic regulation of protein 4.1R involves phosphorylation by cdc2 kinase[J].Mol Biol Cell,2005,16(1):117

相似文献/References:

[1]郭海周))△,张春芳)#,张恒).鼠肺缺血预处理后肺组织中热休克蛋白27检测*[J].郑州大学学报(医学版),2009,(01):81.
 GUO Haizhou),ZHANG Chunfang),ZHANG Heng).Protective effects of heat shock protein 27 in rat lung during ischemic preconditioning[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):81.
[2]高峰),李立),张俊杰),等.重症肌无力患者胸腺组织中共刺激分子B7H1的表达*[J].郑州大学学报(医学版),2012,(03):287.
 GAO Feng),LI Li),ZHANG Junjie),et al.Expression of B7H1 in thymus of patients with myasthenia gravis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(03):287.
[3]杨俊红),崔新征),方华),等.重症肌无力患者胸腺组织抑制性消减cDNA文库的构建及分析*[J].郑州大学学报(医学版),2012,(03):295.
 YANG Junhong),CUI Xinzheng),FANG Hua),et al.Construction and analysis of suppression subtractive cDNA library of thymus from myasthenia gravis patients[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(03):295.
[4]关兵峰),崔新征),方华),等.重症肌无力患者胸腺组织中胆碱乙酰转移酶和烟碱型乙酰胆碱受体的表达*[J].郑州大学学报(医学版),2012,(03):299.
 GUAN Bingfeng),CUI Xinzheng),FANG Hua),et al.Expression of and choline acetyltransferase nicotinic acetylcholine receptor in thymus tissue of myasthenia gravis patients[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(03):299.
[5]崔新征),李立),杨鲲鹏),等.新的重症肌无力外科临床分型和分期的应用*[J].郑州大学学报(医学版),2012,(03):303.
 CUI Xinzheng),LI Li),YANG Kunpeng),et al.Application of a new clinical classification and stages in the surgery of patients with myasthenia gravis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(03):303.

备注/Memo

备注/Memo:
*国家自然科学基金资助项目30570625;河南省医学科技攻关基金资助项目201003147 #通讯作者,男,1963年11月生,主任医师,教授,研究方向:重症肌无力的临床医学与发病机制,Email:qyzhang@zzu.edu.cn
更新日期/Last Update: 2012-05-31