[1]葛丽丽,杨小昂#.肝癌细胞系Huh7中EpCAM+细胞亚群的肿瘤干细胞样特性*[J].郑州大学学报(医学版),2012,(03):306.
 GE Lili,YANG Xiaoang.Cancer stem celllike properties of EpCAM+ cell subpopulation in hepatocellular carcinoma cell line Huh7[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(03):306.
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肝癌细胞系Huh7中EpCAM+细胞亚群的肿瘤干细胞样特性*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年03期
页码:
306
栏目:
论著
出版日期:
2012-05-20

文章信息/Info

Title:
Cancer stem celllike properties of EpCAM+ cell subpopulation in hepatocellular carcinoma cell line Huh7
作者:
葛丽丽杨小昂#
郑州大学医药科学研究院;河南省肝病药理重点实验室 郑州 450052
Author(s):
GE Lili YANG Xiao’ang
Academy of Medical and Pharmaceutical Sciences, Zhengzhou University; Henan Key Laboratory for Pharmacology of Liver Disease,Zhengzhou 450052
关键词:
肝癌肿瘤干细胞上皮细胞黏附分子
Keywords:
liver cancercancer stem cell epithelial cell adhesion molecule
分类号:
R735.7
摘要:
目的:观察从肝癌细胞系Huh7中分选出的EpCAM+细胞亚群的肿瘤干细胞样特性。方法:采用免疫磁珠法从Huh7细胞中分离EpCAM+和EpCAM-细胞亚群,比较其体外增殖、克隆形成、分化、耐药和体内成瘤能力。结果:EpCAM+细胞体外增殖能力强于EpCAM-细胞(P<0.001);EpCAM+细胞克隆形成数高于EpCAM-细胞(P<0.001);分选后随着培养时间的延长,EpCAM+细胞的比率逐渐降低;加入顺铂培养后, EpCAM+细胞亚群的比率增加(P<0.001);1×103个EpCAM+细胞接种裸鼠皮下4 周即可成瘤,而在相同条件下,EpCAM-细胞至少需要接种1×105个和接种更长的时间才能成瘤。结论:EpCAM+细胞亚群具有肝癌干细胞样特性,EpCAM可作为分离纯化肝癌干细胞的一个标志物。
Abstract:
Aim:To observe the properties of epithelial cell adhesion molecule(EpCAM)positive cell subpopulation sorted from human hepatocellular carcinoma cell line Huh7.Methods:EpCAM+ and EpCAM- subpopulations were isolated by automagnetic activated cellsorting system and their abilities of roliferation,differentiation,colony formation,resistance to cisplatin and tumorgenicity were observed.Results:The cell proliferation ability of EpCAM+ subpopulation was stronger than that of EpCAM- subpopulation(P<0.001).The number of colonies formed by EpCAM+ subpopulations was more than that of EpCAM- subpopulations(P<0.001).The proportion of EpCAM+ cells in sorted subpopulation decreased gradually as the cells were cultured. After Huh7 cells were cultured with cisplatin,the percentage of EpCAM+ cells increased(P<0.001).Only 103 EpCAM+ cells were enough to form tumors in nude mice after subcutaneous inoculation for four weeks, while 105 EpCAM- cells were necessary to form tumors.Conclusion:EpCAM+ subpopulation sorted from Huh7 has properties of liver cancer stem cell. EpCAM might be a valuable marker for isolating and purifying hepatocellular carcinoma stem cells.

参考文献/References:

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备注/Memo

备注/Memo:
*河南省基础与前沿计划基金资助项目072300450060;河南省医学攻关计划基金资助项目200703087 #通讯作者,男,1963年5月生,博士,研究员,研究方向:肿瘤免疫学,Email: xiaoangyang@163.com
更新日期/Last Update: 2012-05-31