[1]宋帅召,张东铭,付艳芹,等.高糖高脂饮食妊娠大鼠脂联素和AMPK mRNA的表达*[J].郑州大学学报(医学版),2012,(03):309.
 SONG Shuaizhao,ZHANG Dongming,FU Yanqin,et al.Expression of adiponectin and AMPK mRNA in pregnant rats with high sucrose and fat diet[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(03):309.
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高糖高脂饮食妊娠大鼠脂联素和AMPK mRNA的表达*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年03期
页码:
309
栏目:
论著
出版日期:
2012-05-20

文章信息/Info

Title:
Expression of adiponectin and AMPK mRNA in pregnant rats with high sucrose and fat diet
作者:
宋帅召张东铭付艳芹辛雅萍张苏河#
郑州大学第二附属医院内分泌科 郑州 450014
Author(s):
SONG ShuaizhaoZHANG DongmingFU YanqinXIN YapingZHANG Suhe
Department of Endocrinology,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014
关键词:
胰岛素抵抗脂联素腺苷酸活化蛋白激酶妊娠大鼠
Keywords:
insulin resistanceadiponectinAMPactivated protein kinasegestationrat
分类号:
R587.1
摘要:
目的:探讨高糖高脂饮食妊娠大鼠脂联素(APN)和腺苷酸活化蛋白激酶(AMPK)mRNA的表达与胰岛素抵抗(IR)的关系。方法:将66只健康雌性SD大鼠随机分为高糖高脂饮食孕鼠组(HP组)、高糖高脂饮食未孕鼠组(HV组)、正常饮食孕鼠组(NP组)及正常饮食未孕鼠组(NV组)4组。在妊娠第20天禁食12 h后测空腹血糖和胰岛素水平,计算胰岛素抵抗指数(HOMAIR);应用RTPCR测定脂肪组织中APN mRNA和骨骼肌组织中AMPKα2 mRNA的表达。结果:4组空腹血糖、胰岛素水平和HOMAIR水平差异有统计学意义(P<0.001),HP组空腹血糖、胰岛素水平和HOMAIR较其他3组明显升高(P<0.05);4组APN和AMPKα2 mRNA表达水平差异有统计学意义(P<0.001),HP组APN和AMPKα 2 mRNA的表达较HV组和NP组明显降低(P<0.05),且均与HOMAIR呈负相关(r=-0.537,-0.516;P<0.05)。结论:APN和AMPK可能参与了妊娠期IR的发生发展。
Abstract:
Aim: To study the relationship between the expression of adiponectin and AMPactivated protein kinase(AMPK)and insulin resistance (IR) of pregnant rats with high sucrose and fat diet.Methods:Sixtysix healthy female SD rats were divided into four groups randomly: sucrose and fat diet pregnant rat(HP) group, sucrose and fat diet virgin rat (HV) group, normal diet pregnant rat (NP) group and normal diet virgin rat (NV) group. Twenty days after gestation,fasting plasma glucose (FPG) and fasting insulin (FINS) were measured.IR index (HOMAIR) was calculated according to FPG and FINS. RTPCR was performed to detect the expression of APN mRNA in adipose tissue and AMPK mRNA in the skeletal muscle tissue.Results:Levels of FPG, FINS and HOMAIR showed significant difference in four groups(P<0.001);levels of FPG, FINS and HOMAIR in HP group increased significantly than those in the other three groups(P<0.05).Levels of adiponectin mRNA and AMPK mRNA showed significant difference in four groups(P<0.001);levels of adiponectin mRNA and AMPK mRNA in HP group decreased significantly than those in HV and NP groups (P<0.05).The expression of adiponectin mRNA and AMPK mRNA correlated with HOMAIR negatively in HP group (r=-0.537,-0.516;P<0.05).Conclusion:Adiponectin and AMPK might be involved in the IR in gestation.

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备注/Memo

备注/Memo:
*河南省医学科技攻关计划重点基金资助项目201002010 #通讯作者,女,1956年2月生,博士,教授,主任医师,研究方向:妊娠期糖尿病胰岛素抵抗的相关因素,Email:zhangsuhe@126.com
更新日期/Last Update: 2012-05-31