[1]王丽萍),曾宪旭),牛凤兰),等.五倍子酸对小鼠胃癌MFC和肝癌H22细胞增殖的抑制作用[J].郑州大学学报(医学版),2012,(03):339.
 WANG Liping),ZENG Xianxu),NIU Fenglan),et al.Inhibitive effects of gallic acid on proliferation of murine gastric cancer and hepatoma22 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(03):339.
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五倍子酸对小鼠胃癌MFC和肝癌H22细胞增殖的抑制作用
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年03期
页码:
339
栏目:
论著
出版日期:
2012-05-20

文章信息/Info

Title:
Inhibitive effects of gallic acid on proliferation of murine gastric cancer and hepatoma22 cells
作者:
王丽萍12)曾宪旭3)牛凤兰4)石卓2)#
1)郑州大学基础医学院组织学与胚胎学教研室 郑州 450001 2)吉林大学基础医学院药理学教研室 长春 130021 3)郑州大学第三附属医院病理科 郑州 450052 4)吉林大学公共卫生学院卫生毒理学教研室 长春 130021
Author(s):
WANG Liping12)ZENG Xianxu3)NIU Fenglan4)SHI Zhuo2)
1)Department of Histology and Embryology,College of Basic Medical Sciences,Zhengzhou University, Zhengzhou 450001 2)Department of Pharmacology,College of Basic Medical Sciences,Jilin University, Changchun 130021 3)Department of Pathology, the Third Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 4)Department of Hygienic Toxicology, School of Public Health, Jilin University, Changchun 130021
关键词:
五倍子酸MFC细胞H22细胞小鼠增殖
Keywords:
gallic acid MFC cell H22 cellmouseproliferation
分类号:
R961.1
摘要:
目的:研究五倍子酸(GA)对小鼠胃癌MFC细胞及小鼠肝癌H22细胞增殖的影响。方法:分别用3.125、6.250、12.500、25.000、50.000和100.000 mg/L的GA处理MFC和H22细胞48 h后,应用MTT法检测细胞的增殖抑制率;分别以6.25、12.50和25.00 mg/L的GA作用MFC和H22细胞48 h后,应用流式细胞仪检测细胞周期及凋亡情况;建立移植性H22和MFC荷瘤小鼠模型,分别给予0.50、0.25和0.20 g/kg GA、环磷酰和生理盐水10 d后计算抑瘤率。结果:GA呈剂量依赖性抑制MFC及H22细胞增殖(F=70.845、145.444,P<0.001)。GA使MFC细胞和H22细胞均阻滞在G0/G1期(F=80.432、42.903, P<0.001),各组MFC细胞凋亡率差异有统计学意义(F=45.831,P<0.001)。不同剂量GA对荷MFC和H22瘤小鼠的抑瘤率差异有统计学意义(F=206.264、110.906,P<0.001)。结论:GA具有较好的抑制MFC和H22细胞增殖的作用。
Abstract:
Aim:To explore the effect of gallic acid(GA) on proliferation of murine gastric cancer (MFC) and hepatoma22 (H22) cells.Methods:MTT was employed to detect proliferation ability of MFC and H22 cells treated with 3.125,6.250,12.500,25.000,50.000 and 100.000 mg/L GA for 48 h, respectively. Flow cytometry was used to examine cell cycle progression and apoptotic rate of MFC and H22 cells treated with 6.25,12.50 and 25.00 mg/L GA for 48 h. Tumor inhibition rates were calculated after the mice bearing H22 or MFC cells,which were administered GA(0.50,0.25 and 0.20 g/kg),cyclophosphamide or normal saline for 10 days.Results:The proliferation inhibtion rate of GA on MFC and H22 cells were enhanced in a dosedependant manner(F=70.845,145.444;P<0.001).Both MFC and H22 cells were arrested at G0/G1(F=80.432,42.903;P<0.001) and increase of cellular apoptosis was detected in MFC cells(F=45.831,P<0.001). The antitumor rates of the different dosage of GA for mice bearing MFC and H22 cells had significant differences(F=206.264,110.906;P<0.001).Conclusion:GA is applicable to inhibit proliferation of MFC and H22 cells both in vivo and in vitro mainly.

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备注/Memo

备注/Memo:
#通讯作者,女,1962年7月生,硕士,教授,研究方向:肿瘤药理学,Email:shizhuo@jlu.edu.cn
更新日期/Last Update: 2012-05-31