[1]刘晓莉),刘怀民)#,高启龙),等.靛玉红甲肟对食管癌EC9706细胞增殖、凋亡的影响*[J].郑州大学学报(医学版),2012,(04):429.
 LIU Xiaoli,LIU Huaimin,GAO Qilong,et al.Effects of indirubin3’monoxime on proliferation and apoptosis of human esophageal cancer EC9706 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(04):429.
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靛玉红甲肟对食管癌EC9706细胞增殖、凋亡的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年04期
页码:
429
栏目:
食管癌研究
出版日期:
2012-07-20

文章信息/Info

Title:
Effects of indirubin3’monoxime on proliferation and apoptosis of human esophageal cancer EC9706 cells
作者:
刘晓莉1)刘怀民1)#高启龙1)陈小兵2)张成娟3)杨峰1)
1)河南省肿瘤医院中西医结合科 郑州 450008 2)河南省肿瘤医院肿瘤内科 郑州 450008 3)河南省肿瘤医院病理科 郑州 450008
Author(s):
LIU Xiaoli1)LIU Huaimin1)GAO Qilong1)CHEN Xiaobing2)ZHANG Chengjuan3)YANG Feng1)
1)Department of Traditional Chinese and Western Medicine,Henan Province Tumor Hospital,Zhengzhou 450008 2)Department of Medical Oncology,Henan Province Tumor Hospital,Zhengzhou 450008 3)Department of Pathology,Henan Province Tumor Hospital,Zhengzhou 450008
关键词:
食管肿瘤靛玉红甲肟Sox2增殖凋亡EC9706
Keywords:
esophageal neoplasmindirubin3’monoximeSox2proliferationapoptosisEC9706
分类号:
R735.1
摘要:
目的:探讨靛玉红甲肟对食管癌EC9706细胞增殖和凋亡的影响。方法:分别用0、1、5和10 μmol/L靛玉红甲肟处理EC9706细胞12、24和48 h后,采用MTT法检测细胞增殖抑制率;用10 μmol/L靛玉红甲肟处理EC9706细胞0、24、48和72 h后,采用流式细胞术法检测凋亡率;分别用0、1、5和10 μmol/L靛玉红甲肟处理EC9706细胞48 h后,采用RTPCR法检测细胞Sox2 mRNA的表达。结果:靛玉红甲肟对食管癌EC9706细胞增殖具有明显的抑制作用,且表现为剂量依赖性和时间依赖性(F剂量=135.237,F时间=96.488,F交互=189.544,P均<0.001)。靛玉红甲肟作用EC9706细胞后,细胞凋亡率呈时间依赖性上升(F=195.350,P<0.001),Sox2 mRNA的表达呈剂量依赖性下调(F=87.552,P<0.001)。结论:靛玉红甲肟对食管癌EC9706细胞具有明显的增殖抑制和诱导凋亡作用,其作用机制可能与下调Sox2 mRNA表达有关。
Abstract:
Aim: To explore the effects of indirubin3’monoxime on proliferation and apoptosis of esophageal cancer EC9706 cells.Methods:EC9706 cells were treated with 0,1,5,and 10 μmol/L indirubin3’monoxime for 12,24 and 48 h,then the proliferative inhibition rate was measured by MTT assay.EC9706 cells were treated with 10 μmol/L indirubin3’monoxime for 0,24,48, and 72 h,then the apoptosis rate was measured by flow cytometry.EC9706 cells were treated with 0,1,5,and 10 μmol/L indirubin3’monoxime for 48 h,then the expression of Sox2 mRNA was analyzed by RTPCR.Results:Indirubin3’monoxime markedly inhibited the proliferation of EC9706 cells in a dose and timedependent manner(Fconcentration=135.237,Ftime=96.488,Finteraction=189.544,P<0.001).The apoptosis rate increased in a timedependent manner(F=195.350,P<0.001),and Sox2 mRNA was downregulated in a dosedependent manner(F=87.552,P<0.001) after the treatment by indirubin3’monoxime.Conclusion:Indirubin3’monoxime could inhibit the proliferation and induce apoptosis of EC9706 cells through downregulating Sox2 mRNA expression.

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备注/Memo

备注/Memo:
*河南省医学科技攻关计划基金资助项目2011020156 #通讯作者,男,1970年3月生,博士,副主任医师,研究方向:消化道肿瘤的中西医结合治疗,Email:lhm1970311@126.com
更新日期/Last Update: 2012-08-14