[1]宋中立),刘海东),王祚懿),等.重铬酸钾对大鼠睾丸支持细胞中雄激素结合蛋白、转铁蛋白和抑制素蛋白表达的影响[J].郑州大学学报(医学版),2012,(04):479.
 SONG Zhongli,LIU Haidong,WANG Zuoyi,et al.Effect of potassium bichromate on expressions of ABP,Tf and INH in rat sertoli cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(04):479.
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重铬酸钾对大鼠睾丸支持细胞中雄激素结合蛋白、转铁蛋白和抑制素蛋白表达的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年04期
页码:
479
栏目:
论著
出版日期:
2012-07-20

文章信息/Info

Title:
Effect of potassium bichromate on expressions of ABP,Tf and INH in rat sertoli cells
作者:
宋中立1)刘海东2)王祚懿1)李时恩1)#陈小玉1)姚武1)吴逸明1)
1)郑州大学公共卫生学院劳动卫生学教研室 郑州 450001 2)乳山市疾病预防控制中心 乳山 264500
Author(s):
SONG Zhongli1)LIU Haidong2)WANG Zuoyi1)LI Shi’en1)CHEN Xiaoyu1)YAO Wu1)WU Yiming1)
1)Department of Occupational Health,College of Public Health,Zhengzhou University,Zhengzhou 450001 2)Rushan Center for Disease Control and Prevention,Rushan 264500
关键词:
重铬酸钾支持细胞雄激素结合蛋白转铁蛋白抑制素大鼠
Keywords:
potassium bichromatesertoli cellandrogen binding proteinTfinhibinrat
分类号:
R136.3
摘要:
目的:研究重铬酸钾对大鼠睾丸支持细胞中雄激素结合蛋白(ABP)、转铁蛋白(Tf) 和抑制素(INH)蛋白的表达的影响。方法:建立大鼠睾丸支持细胞体外原代培养模型,分为0 mol/L、10-7 mol/L、10-6 mol/L重铬酸钾染毒组,Western blot法检测重铬酸钾作用24 h后各组睾丸支持细胞中ABP、Tf和INH蛋白表达水平的变化。结果:ABP蛋白的表达量随重铬酸钾浓度的升高而升高(0.39±0.19、0.51±0.18及0.81±0.19),差异有统计学意义(F=10.738,P<0.001);Tf蛋白的表达量也随重铬酸钾浓度的升高而增加(0.56±0.19、1.21±0.19及1.19±0.17),差异有统计学意义(F=32.435,P<0.001);而INH蛋白表达却呈下降趋势(1.06±0.19、1.20±0.19及0.71±0.19),差异有统计学意义(F=14.116,P<0.001)。结论:重铬酸钾能够引起大鼠睾丸支持细胞分泌功能的变化,产生生殖毒性作用。
Abstract:
Aim:To observe the effect of potassium bichromate on the expressions of ABP,Tf and INH protein in rat sertoli cells(SC).Methods:Rat SC were isolated and cultured with 0 mol/L,10-7 mol/L,10-6 mol/L potassium bichromate for 24 h.Western blot was used to determine the expressions of ABP,Tf and INH protein.Results:With the increasing concentration of potassium bichromate, the expression level of ABP(0.39±0.19,0.51±0.18,and 0.81±0.19;F=10.738,P<0.001) and Tf protein(0.56±0.19,1.21±0.19,and 1.19±0.17;F=32.435,P<0.001) increased;while that of INH protein decreased(1.06±0.19,1.20±0.19,and 0.71±0.19;F=14.116,P<0.001).Conclusion: Potassium bichromate can affect the secretory functions of rat SC,and produce reproductive toxicity.

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备注/Memo

备注/Memo:
#通讯作者,男,1955年2月生,本科,副教授,研究方向:工业毒理,Email:shienli369@126.com
更新日期/Last Update: 2012-08-14