[1]王琰),陈旻静)#,张娟),等.皮肤恶性黑色素瘤组织中Kiss1和MMP9蛋白的表达[J].郑州大学学报(医学版),2012,(04):481.
 WANG Yan,CHEN Minjing,ZHANG Juan,et al.Expressions of Kiss1 and MMP9 in cutaneous malignant melanoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(04):481.
点击复制

皮肤恶性黑色素瘤组织中Kiss1和MMP9蛋白的表达
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年04期
页码:
481
栏目:
论著
出版日期:
2012-07-20

文章信息/Info

Title:
Expressions of Kiss1 and MMP9 in cutaneous malignant melanoma tissue
作者:
王琰12)陈旻静1)#张娟12)王大伟12)
1)郑州大学第一附属医院整形外科 郑州 450052 2)河南省高等学校临床医学重点学科开放实验室 郑州 450052
Author(s):
WANG Yan12)CHEN Minjing1)ZHANG Juan12)WANG Dawei12)
1)Department of Plastic Surgery, the First Affiliated Hospital,Zhengzhou University, Zhengzhou 450052 2)Open Laboratory of Key Discipline of Clinical Medical Sciences, Higher School of Henan Province, Zhengzhou 450052
关键词:
皮肤恶性黑色素瘤Kiss1基质金属蛋白酶9淋巴结转移
Keywords:
cutaneous malignant melanomaKiss1MMP9lymph node metastasis
分类号:
R739.5
摘要:
目的:检测皮肤恶性黑色素瘤(CMM)组织中Kiss1和基质金属蛋白酶9(MMP9)的表达。方法:应用免疫组化SP法检测42例CMM、20例皮肤交界痣及20例正常皮肤组织中Kiss1及MMP9蛋白的表达,探讨其与CMM发生及浸润转移的关系。结果:3种组织中Kiss1和MMP9蛋白阳性表达率相比差异有统计学意义(χ2=14.474和12.866,P<0.05);CMM组织中Kiss1的表达低于交界痣及正常皮肤组织,而MMP9的表达高于交界痣及正常皮肤组织(P<0.017)。CMM组织中Kiss1及MMP9的表达均与肿瘤的浸润深度及淋巴结转移有关(P<0.05)。CMM组织中Kiss1与MMP9的表达呈负关联(rP=-0.342,P=0.026)。结论:Kiss1和MMP9在CMM的发生发展中起重要作用。
Abstract:
Aim:To detect the expressions of Kiss1 and MMP9 in cutaneous malignant melanoma(CMM) tissue.Methods:The expressions of Kiss1 and MMP9 in 42 cases of CMM, 20 cases of junctional nevus and 20 cases of normal skin specimens were detected by immunohistochemical SP technique.Results:The positive expression rates of Kiss1 and MMP9 among the 3 groups had significant difference(χ2=14.474,12.866,P<0.05).The positive expression rate of Kiss1 protein in CMM tissue was lower than those in junctional nevus and normal skin tissue(P<0.017),but the positive expression rate of MMP9 protein in CMM tissue was significantly higher than those in junctional nevus and normal skin tissue(P<0.017).The expressions of Kiss1 and MMP9 in CMM tissue was related to the invasion depth of tumor and lymph node metastasis(P<0.05). The expressions of Kiss1 and MMP9 in CMM tissue was negatively associated(rP=-0.342,P=0.026).Conclusion:Kiss1 and MMP9 may play an important role in the carcinogenesis and development of CMM.

参考文献/References:

[1]Lee JH,Welch DR.Suppression of metastasis in human breast carcinoma MDAMB435 cells after transfection with the metastasis suppressor gene,Kiss1[J].Cancer Res,1997,57(12):2384
[2]Ikeguchi M,Yamaguchi K,Kaibara N.Clinical significance of the loss of Kiss1 and orphan Gproteincoupled receptor (hOT7T175)gene expression in esophageal squamous cell carcinoma[J]. Clin Cancer Res,2004,10(4):1379
[3]Dhar DK,Naora H,Kubota H,et al. Downregulation of Kiss1 expression is responsible for tumor invasion and worse prognosis in gastric carcinoma[J].Int J Cancer,2004,111(6):868
[4]Stark AM,Tongers K,Maass N,et al.Reduced metastasissuppressor gene mRNAexpression in breast cancer brain metastases[J].J Cancer Res Clin Oncol,2005,131(3):191
[5]张祖平,郭爱萍,王玉龙,等.MMP9在乳腺癌中的表达及临床意义[J].中国肿瘤临床, 2002, 29(1):72
[6]刘易欣,赵春歌,赵秀兰,等.MMP9 EGFR在胃癌Lauren’s分型中的表达及意义[J]. 中国肿瘤临床,2006,33(11):632
[7]Masui T,Doi R,Mori T, et al. Metastin and its variant forms suppress migration of pancreatic cancer cells[J].Biochem Biophys Res Commun,2004,315(1):85
[8]李卫东,姚庆娟,刘刚,等.结直肠癌VEGF和MMP9基因的表达[J].中国肿瘤临床, 2008, 35(13):756
[9]黄海力,王孟薇.肿瘤浸润转移分子机制的研究进展[J].生物技术通信,2006,17(1):84
[10]Lee JH,Miele ME,Hicks DJ,et al.Kiss1,a novel human malignant melanoma metastasissuppressor gene[J].J Natl Cancer Inst,1996,88(23):1731
[11]Kotani M,Detheux M,Vandenbogaerde A,et al.The metastasis suppressor gene KISS1 encodes Kisspeptins,the natural ligands of the orphan G proteincoupled receptor GPR54[J].J Biol Chem,2001,276(37):34631
[12]Stafford LJ,Xia C,Ma W,et al.Identification and characterization of mouse metastasis suppressor KISS1 and its Gproteincoupled receptor[J].Cancer Res,2002,62(19):5399
[13]Becker JA,Mirjolet JF,Bernard J,et al.Activation of GPR54 promotes cell cycle arrest and apoptosis of human tumor cells through a specific transcriptional program not shared by other Gqcoupled receptors[J].Biochem Biophys Res Commun,2005,326(3):677
[14]张延新,刘喜民,陈奎生,等.胃癌组织中KISS1和基质金属蛋白酶9蛋白的表达[J]. 郑州大学学报:医学版,2010,45(3):384
[15]李娜,李珊珊,张红艳,等.食管鳞状细胞癌组织中KISS1和MMP9蛋白和mRNA的表达[J]. 郑州大学学报:医学版,2009,44(1):55
[16]Yan C,Wang H,Boyd DD. Kiss1 represses 92kDa typeⅣ collagenase expression by down regulating NFκB binding to the promoter as a consequence of IκBαinduced block of p65/p50 nuclear translocation[J].J Biol Chem,2001,276(2):1164

相似文献/References:

[1]陈妍琼),赵志华),张蕾)#,等.食管鳞状细胞癌组织中KiSS1蛋白和微血管密度的检测*[J].郑州大学学报(医学版),2009,(06):1127.
 CHEN Yanqiong),ZHAO Zhihua),ZHANG Lei),et al.Expressions of KiSS1 protein and detection of MVD in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):1127.
[2]李娜)),李珊珊)#,张红艳),等.食管鳞状细胞癌组织中KISS1和MMP9蛋白和mRNA的表达*[J].郑州大学学报(医学版),2009,(01):55.
 LI Na),LI Shanshan),ZHANG Hongyan),et al.Expressions of KISS1 and MMP9 in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):55.
[3]张延新),刘喜民),陈奎生)#,等.胃癌组织中KISS1和基质金属蛋白酶9蛋白的表达*[J].郑州大学学报(医学版),2010,(03):384.
 ZHANG Yanxin),LIU Ximin),CHEN Kuisheng),et al.Expression of KISS1 and MMP9 in gastric carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(04):384.
[4]陈旻静,张娟,陈建华.皮肤恶性黑色素瘤组织中Runx3与Smad4蛋白的表达[J].郑州大学学报(医学版),2010,(06):980.

备注/Memo

备注/Memo:
#通讯作者,女,1967年10月生,硕士,教授,主任医师,研究方向:体表肿瘤的基础与临床,Email:chenmj67@yahoo.com.cn
更新日期/Last Update: 2012-08-14