[1]赵意华),陈清汉)﹟,华海婴),等.氯化锂对人骨肉瘤细胞U2OS增殖、凋亡及Fas、Caspase3 mRNA表达的影响[J].郑州大学学报(医学版),2012,(04):502.
 ZHAO Yihua,CHEN Qinghan,HUA Haiying,et al.Effect of lithium chloride on proliferation,apoptosis and mRNA expressions of Fas and Caspase3 in human osteosarcoma U2OS cell[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(04):502.
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氯化锂对人骨肉瘤细胞U2OS增殖、凋亡及Fas、Caspase3 mRNA表达的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年04期
页码:
502
栏目:
论著
出版日期:
2012-07-20

文章信息/Info

Title:
Effect of lithium chloride on proliferation,apoptosis and mRNA expressions of Fas and Caspase3 in human osteosarcoma U2OS cell
作者:
赵意华1)陈清汉1)华海婴2)姜玉军2)秦建英1)任明明1)
1)郑州大学第二附属医院骨科 郑州 450014 2)郑州大学医药科学研究院 郑州 450052
Author(s):
ZHAO Yihua1)CHEN Qinghan1)HUA Haiying2)JIANG Yujun2)QIN Jianying1)REN Mingming1)
1)Department of Orthopaedic Surgery,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014 2)Academy of Medical and Pharmaceutical Sciences,Zhengzhou University,Zhengzhou 450052
关键词:
氯化锂U2OS细胞增殖凋亡骨肉瘤
Keywords:
lithium chlorideU2OS cellproliferationapoptosisosteosarcoma
分类号:
R738.1
摘要:
目的:研究氯化锂(LiCl)对人骨肉瘤细胞U2OS增殖及凋亡的影响,并探讨其可能的机制。方法:以40、80、150 mmol/L的LiCl作用于U2OS细胞24、48和72 h后,采用 MTT法检测细胞增殖抑制率;作用48 h后,流式细胞术法检测细胞凋亡率,并分析细胞周期;RTPCR法检测凋亡相关基因Fas、Caspase3 mRNA的表达。结果:随LiCl作用剂量的增加,U2OS细胞增殖抑制率增加,细胞凋亡率和Fas、Caspase3 mRNA表达量亦增加(F=61 157.480,70.828和418.072,P<0.001);细胞周期分析显示细胞被阻滞于S期(P<0.001)。结论:LiCl可能通过促进凋亡基因Fas、Caspase3的表达,抑制U2OS细胞增殖并诱导凋亡。
Abstract:
Aim:To investigate the effect of lithium chloride(LiCl) on proliferation and apoptosis of human osteosarcoma cell U2OS,and probe the possible mechanism.Methods:U2OS cells were cultured with different concentrations of LiCl(40,80,and 150 mmol/L) for 24,48,and 72 h, respectively, then the growth inhibitory effects were detected with MTT.Apoptosis and cell cycle was observed by flow cytometry,and Fas and Caspase3 mRNA expression,by RTPCR for cells cultured for 48 h.Results:LiCl could inhibit the growth of U2OS cells,induce apoptosis(F=61 157.480,P<0.001) in a dosedependent manner.The cell cycle analysis revealed that the cells were arrested in S checkpoint. LiCl could increase the expression of Fas and Caspase3 mRNA obviously(F=70.828,418.072,P<0.001).Conclusion: LiCl could inhibit the proliferation and induce apoptosis of U2OS cells through increasing the expression of Fas and Caspase3.

参考文献/References:

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备注/Memo

备注/Memo:
#通讯作者,男,1963年5月生,硕士,教授,研究方向:骨科疾病的基础与临床,Email:chenqinghan833@medmail.com.cn
更新日期/Last Update: 2012-08-14