[1]孙鹏辉,梁守沛,陆皓泉,等.肺癌组织热休克蛋白70提取物对A549细胞增殖的影响*[J].郑州大学学报(医学版),2012,(05):608.
 SUN Penghui,LIANG Shoupei,LU Haoquan,et al.Effect of heat shock protein 70 peptide complex on proliferation of A549 cell line[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(05):608.
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肺癌组织热休克蛋白70提取物对A549细胞增殖的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年05期
页码:
608
栏目:
论著
出版日期:
2012-09-20

文章信息/Info

Title:
Effect of heat shock protein 70 peptide complex on proliferation of A549 cell line
作者:
孙鹏辉梁守沛陆皓泉李杰王小龙许东姚武吴逸明周舫#
郑州大学公共卫生学院劳动卫生学教研室 郑州 450001
Author(s):
SUN PenghuiLIANG ShoupeiLU HaoquanLI JieWANG XiaolongXU DongYAO WuWU YimingZHOU Fang
Department of Occupational Health, College of Public Health, Zhengzhou University, Zhengzhou 450001
关键词:
热休克蛋白70肺癌增殖A549细胞
Keywords:
heat shock protein 70lung cancerproliferationA549 cell
分类号:
R730.1
摘要:
目的:观察肺癌组织热休克蛋白70(HSP70)提取物对A549细胞增殖的影响。方法:从肺癌组织中提取、纯化HSP70多肽复合物,分别以0.1、0.5、1.0、2.5及5.0 mg/L作用于A549细胞,采用MTT法检测作用24、48及72 h后细胞的增殖。结果:HSP70多肽复合物可促进A549细胞增殖,其作用随浓度和时间的增加呈增强的趋势(F浓度=171.826,F时间=35.933,P<0.001)。结论:HSP70多肽复合物可以促进A549细胞的增殖。
Abstract:
Aim: To observe the effect of heat shock protein 70(HSP70) peptide complex(HSP70PC) on the proliferation of A549 cells.Methods:Tumorderived HSP70PC was separated and purified from human lung cancer tissue by fast protein liquid chromatography,then A549 cells were treated with HSP70PC at doses of 0.1,0.5,1.0,2.5 and 5.0 mg/L.MTT was used to observe cell proliferation after 24,48 and 72 h.Results:HSP70PC could prompt the proliferation of A549 cells in a doseand timedependent manner (Fdose=171.826,Ftime=35.933,P<0.001).Conclusion: HSP70PC can promote A549 cell proliferation.

参考文献/References:

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目30600490,30571552;河南省教育厅科技创新人才支持计划基金资助项目2008HASTIT028;河南省教育厅自然科学研究资助项目2008A320018;2012年度河南省教育厅科学技术研究重点项目12B330008 #通讯作者,女,1974年7月生,博士,教授,研究方向:职业肿瘤和应激医学,Email:zhoufang23@sina.com
更新日期/Last Update: 1900-01-01