[1]罗向阳,张振武,曹振杰,等.宫内抗雄性物质暴露对胎鼠及新生大鼠阴茎组织Shh及其受体Ptch1 mRNA表达的影响*[J].郑州大学学报(医学版),2012,(05):645.
 LUO Xiangyang,ZHANG Zhenwu,CAO Zhenjie,et al.Intraamniotic androgen exposure on expressions of Shh and Ptch1 mRNA in embryo and preterm rat genital tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(05):645.
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宫内抗雄性物质暴露对胎鼠及新生大鼠阴茎组织Shh及其受体Ptch1 mRNA表达的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年05期
页码:
645
栏目:
论著
出版日期:
2012-09-20

文章信息/Info

Title:
Intraamniotic androgen exposure on expressions of Shh and Ptch1 mRNA in embryo and preterm rat genital tissue
作者:
罗向阳张振武曹振杰王伟#
郑州大学第三附属医院泌尿外科 郑州 450052
Author(s):
LUO Xiangyang ZHANG Zhenwu CAO Zhenjie WANG Wei
Department of Urology,the Third Affiliated Hospital,Zhengzhou University, Zhengzhou 450052
关键词:
ShhPtch1增殖尿道下裂大鼠
Keywords:
sonic hedgehog Ptch1 proliferation hypospadiasrat
分类号:
R726.9
摘要:
目的:观察宫内抗雄性物质暴露对胎鼠及新生大鼠阴茎组织Shh及其受体Ptch1 mRNA表达的影响。方法:SD妊娠大鼠随机分为氟他胺组(Flu组)和生理盐水组(对照组),分别于孕12~16 d腹部皮下注射Flu或生理盐水100 mg/(kg•d),2组均于胚胎19 d及出生后1、4、7 d各随机取8只大鼠,应用RTPCR技术检测各组胎鼠及新生大鼠阴茎组织Shh基因及其受体Ptch1的表达。结果:2组动物Shh mRNA的表达在孕19 d~出生后4 d均随鼠龄增加表达逐渐增强,但Flu组在孕19 d及出生后1、4、7 d均低于对照组(P<0.05)。对照组Ptch1 mRNA的表达在孕19 d~出生后7 d随鼠龄增加无变化(F=3.231,P=0.143),而Flu组有增强的趋势(F=8.922,P=0.030),且Flu组在孕19 d及出生后1、7 d与对照组相比差异有统计学意义(P<0.05)。结论:宫内抗雄性物质暴露可能通过调控Shh基因及其受体Ptch1的表达引起阴茎组织发生发展中尿道襞间充质细胞与上皮细胞转化异常,尿道板腔化受阻,进而导致尿道下裂的发生。
Abstract:
Aim:To investigate the expressions of sonic hedgehog(Shh) and Ptch1 mRNA in genital tissue of embryo and preterm rats exposed to intraamniotic androgen.Methods:Pregnant SD rats were randomly divided into two groups:normal saline group and flutamide(Flu) group.Normal saline or Flu were injected into the rats from gestational days 12 to 16,respectively.The genital tissue from the 2 groups on gestational day 19 and postnatal day 1,4,and 7 was removed. Shh and Ptch1 mRNA levels were measured by semiquantitative reverse transcription PCR.Results:The level of Shh mRNA increased in both two groups from gestational day 19 to postnatal day 4. However, in Flu group,it had significant difference from controls on gestational day 19 and postnatal days 1,4 and 7(P<0.05).The level of Ptch1 mRNA in Flu group increased from gestational day 19 to postnatal day 7(F=8.922,P=0.030),which had significant differences from controls on gestational day 19,and postnatal day 1 and 7(P<0.05).Conclusion:The results suggest Flu exposure regulate the expressions of Shh and Ptch1 mRNA,and perhaps contribute to the pathogenesis of hypospadias.

参考文献/References:

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备注/Memo

备注/Memo:
*河南省卫生科技创新型人才工程专项经费资助项目 #通讯作者,女,1973年10月生,博士,副教授,研究方向:儿童遗传代谢内分泌,Email:weiwang169@yahoo.com.cn
更新日期/Last Update: 1900-01-01