[1]何文强△,尹继云,孟晓,等.siRNA沉默Pim3对T24细胞增殖和细胞周期的影响[J].郑州大学学报(医学版),2012,(05):681.
 HE Wenqiang,YIN Jiyun,MENG Xiao,et al.Effect of silencing Pim3 with small interfering RNA on cell proliferation and cell cycle of T24 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(05):681.
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siRNA沉默Pim3对T24细胞增殖和细胞周期的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年05期
页码:
681
栏目:
论著
出版日期:
2012-09-20

文章信息/Info

Title:
Effect of silencing Pim3 with small interfering RNA on cell proliferation and cell cycle of T24 cells
作者:
何文强△尹继云孟晓王梅叶张文涛曲宪东李南南
武警河南总队医院泌尿外科 郑州 450052
Author(s):
HE WenqiangYIN JiyunMENG XiaoWANG MeiyeZHANG WentaoQU XiandongLI Nannan
Departmen of Urology,Henan Provincial Cops Hospital,Chinese People’s Armed Police Force,Zhengzhou 450052
关键词:
Pim3膀胱癌T24细胞小干扰RNA细胞增殖细胞周期
Keywords:
Pim3bladder carcinomaT24 cellsmall interfering RNAcell proliferationcell cycle
分类号:
R737.14
摘要:
目的:研究小干扰RNA(siRNA)沉默Pim3对膀胱癌细胞增殖和周期的影响。方法:膀胱癌T24细胞分为3组:未处理组(不作任何处理)、对照siRNA组(采用50 nmol/L对照siRNA转染)和Pim3 siRNA组(采用50 nmol/L Pim3 siRNA转染)。采用Western blot检测转染48 h Pim3、Cyclin D1、Cdk2、P21蛋白表达的变化,CCK8试剂检测转染24、48、72、96 h细胞的增殖速率,流式细胞术检测转染48 h细胞周期的变化。结果:3组膀胱癌T24细胞Pim3、Cyclin D1、Cdk2、P21蛋白表达比较,差异有统计学意义(F=32.506、49.573、23.079、44.758,P<0.05),与未处理组和对照siRNA组相比,Pim3 siRNA组Pim3、Cyclin D1和Cdk2蛋白的表达下降,P21蛋白表达升高(P<0.05)。转染Pim3 siRNA48、72和96 h后,膀胱癌T24细胞的增殖速率均明显受到抑制(F=50.067、132.504、277.389,P<0.001)。3组G0/G1、S和G2/M期膀胱癌T24细胞比例比较,差异有统计学意义(F=107.970、20.039和66.872, P<0.05),Pim3 siRNA组G0/G1期膀胱癌T24细胞比例高于未处理组和对照siRNA组(P<0.05),而S和G2/M期膀胱癌T24细胞比例低于未处理组和对照siRNA组(P<0.05)。结论:Pim3有望成为治疗膀胱癌潜在的分子靶点。
Abstract:
Aim:To investigate the effect of silencing Pim3 with small interfering RNA(siRNA)on cell proliferation and cell cycle of bladder carcinoma T24 cells.Methods:T24 cells were divided into 3 groups:untreated group,control siRNA group,and Pim3 siRNA group.Pim3 siRNA and control siRNA were utilized to transfect bladder carcinoma T24 cells by LipofectamineTM2000. Western blot was used to detect the protein expressions of Pim3,Cyclin D1,Cdk2,and P21. CCK8 kit and flow cytometry were used to examine cell proliferation and cell cycle, respectively.Results:The protein expressions of Pim3,Cyclin D1,Cdk2,and P21 among the 3 groups had significant differences(F=32.506,49.573,23.079,44.758,P<0.05). The expressions of Pim3,Cyclin D1,and Cdk2 decreased and P21 increased in Pim3 siRNA group(P<0.05).The proliferation rate of T24 cells was obviously inhibited at 48,72,96 h after transfection with Pim3 siRNA (F=50.067,132.504,277.389,P<0.001).The percentage of G0/G1,S,and G2/M phases cells among the 3 groups had significant differences(F=107.970,20.039,66.872,P<0.05).The percentage of G0/G1 phase of T24 cells in Pim3 siRNA group was higher than those in untreated group and control siRNA group, whereas S and G2/M phases appeared the opposite result.Conclusion:Pim3 may be a useful molecular target for therapy of bladder carcinoma.

参考文献/References:

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备注/Memo

备注/Memo:
△男,1970年6月生,硕士,副主任医师,研究方向:泌尿系肿瘤,Email:qiangwen0519@163.com
更新日期/Last Update: 1900-01-01