[1]赵玉玲,马晓光,李辉,等.河南省尉氏县结核分枝杆菌的基因型流行特征及耐药性*[J].郑州大学学报(医学版),2012,(05):691.
 ZHAO Yuling,MA Xiaoguang,LI Hui,et al.Genotyping characteristic and drug resistance of Mycobacterium tuberculosis isolated in Weishi county of Henan province[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(05):691.
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河南省尉氏县结核分枝杆菌的基因型流行特征及耐药性*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年05期
页码:
691
栏目:
应用研究
出版日期:
2012-09-20

文章信息/Info

Title:
Genotyping characteristic and drug resistance of Mycobacterium tuberculosis isolated in Weishi county of Henan province
作者:
赵玉玲马晓光李辉徐吉英#闫国蕊石洁
河南省疾病预防控制中心结核病预防控制所 郑州 450016
Author(s):
ZHAO YulingMA XiaoguangLI HuiXU JiyingYAN GuoruiSHI Jie
Institution of Tuberculosis Prevention and Control,Henan Provincial Center for Disease Control and Prevention,Zhengzhou 450016
关键词:
结核分枝杆菌基因分型多位点串联重复河南省耐药性
Keywords:
Mycobacterium tuberculosisgenotypingvariable number tandem repeatsHenan provincedrug resistance
分类号:
R183.3
摘要:
目的:探讨河南省尉氏县结核分枝杆菌的可变数目串联重复序列(VNTR)基因型流行特征和耐药情况。方法:河南省尉氏县结核病防治机构分离培养的257株结核分枝杆菌,采用比例法药敏试验检测其耐药情况。应用RD105缺失基因检测法鉴定北京家族基因型菌株,7个结核分枝杆菌VNTR基因位点对结核分枝杆菌进行分型,并对其分型结果进行聚类分析。结果:北京家族基因型占90.7%(233/257);VNTR基因型显示明显的多态性。230个基因型聚类分析主要分为4个基因群(Ⅰ群、Ⅱ群、Ⅲ群和Ⅳ群),其中Ⅲ群包含152基因型(59.1%),为优势基因型;耐多药结核分枝杆菌占8.9%(23/257),任意耐药占24.5%(63/257),北京家族基因型与非北京家族基因型菌株以及主要流行(Ⅲ)群与非主要流行群中耐药株分布比较,差异无统计学意义(χ2=2.412、1.954,P>0.05)。结论:河南省尉氏县北京家族基因型结核分枝杆菌为主导流行型;7位点VNTR基因分型存在4个基因群,主要流行群为Ⅲ群;耐药形势仍不容乐观。
Abstract:
Aim:To investigate the genotyping characteristic and drug resistance of the variable number tandem repeats(VNTR) of Mycobacterium tuberculosis clinical isolates in Weishi,Henan province with multilocus VNTR analysis preliminarily.Methods:A total of 257 Mycobacterium tuberculosis clinical isolates selected from Weishi county randomly were cultured with LowensteinJensen medium from June 2009 to June 2011.The drug susceptibility of Mycobacterium tuberculosis was determined by proportion method. The genotypes were identified by RD105 detection test and the polymorphism of 7 VNTR locus.The clustering of genotype was analyzed with BioNumerics.Results:Beijing family genotype accounted for 90.7%(233/257) by RD105 detection test.The VNTR genotypes showed obvious polymorphisms.The clustering of genotype showed that these strains could be categorized into 4 gene clusters(Ⅰ,Ⅱ,Ⅲ and Ⅳ)and 230 genotypes.Among them, 59.1% were cluster Ⅲ including 152 genotypes,which was the dominant genotype. The drug resistant result showed that there were 23 MDRTB strains,accounting for 8.9%(23/257),any there were 63 drug resistant strains,accounting for 24.5%(63/257). The drug resistant strains distribution between Beijing family genotype and nonBeijing family genotype,as well as major population and non major population had significant differences(χ2=2.412 and 1.954,P>0.05).Conclusion:There are at least 4 clusters of VNTR genotype and cluster Ⅲ is the predominant prevalent strain in Weishi county. The drug resistance is still not optimistic.

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备注/Memo

备注/Memo:
*国家“十?一五”科技重大传染病专项基金资助项目2008ZX10003010;河南省医学科技攻关计划基金资助项目201003140 #通讯作者,女,1965年5月生,本科,主任医师,研究方向:结核、HIV双重感染,Email:xjy3115@sohu.com
更新日期/Last Update: 1900-01-01