[1]沈洁,冯常炜#,郝炳章,等.血清中miR21和let7a在胃癌无创性诊断及手术效果评定中的作用[J].郑州大学学报(医学版),2012,(05):722.
 SHEN Jie,FENG Changwei,HAO Bingzhang,et al.Application of miR21 and let7a in serum for noninvasive diagnosis of gastric cancer and evaluation of surgery results[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(05):722.
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血清中miR21和let7a在胃癌无创性诊断及手术效果评定中的作用
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年05期
页码:
722
栏目:
应用研究
出版日期:
2012-09-20

文章信息/Info

Title:
Application of miR21 and let7a in serum for noninvasive diagnosis of gastric cancer and evaluation of surgery results
作者:
沈洁冯常炜#郝炳章梅璐
郑州大学第二附属医院消化内科 郑州 450014
Author(s):
SHEN JieFENG ChangweiHAO BingzhangMei Lu
Department of Gastroenterology,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014
关键词:
胃癌血清微小RNAmiR21let7a
Keywords:
gastric cancermiR in serummiR21let7a
分类号:
R735.2
摘要:
目的:探讨血清中miR21和let7a在胃癌无创性诊断以及手术效果评定中的作用。方法:采用荧光定量PCR法分别检测正常对照、胃窦溃疡、胃窦炎性息肉、肠上皮化生及胃窦腺癌患者血清样本及配对胃组织中miR21 和let7a的表达。结果:血清中miR21和let7a的含量与配对组织中二者的表达量呈正相关(rS=0.826和0.889,P<0.001)。术后胃癌患者血清中miR21含量较术前降低(t=0.511,P=0.030),而let7a则升高(t=0.770,P<0.001)。5组对象血清中miR21和let7a含量差异均有统计学意义(χ2=10.841,P=0.028;χ2=26.460,P<0.001)。与正常对照组相比,胃癌患者血清中miR21含量升高,肠化及胃癌患者血清中let7a含量降低。结论:血清中miR21和let7a的含量可能成为胃癌的无创诊断及手术效果评定的一种新的有效的生物标记。
Abstract:
Aim:To explore the application of miR21 and let7a in serum for noninvasive on diagnosis of gastric cancer and evaluation of surgery results.Methods:Fluorescence quantitative PCR was used to determine the levels of miR21 and let7a in serum and corresponding tissue samples taken from normal controls, and patients with gastric ulcer, gastric inflammatory polyps, intestinal metaplasia and gastric cancer.Results: miR21 and let7a in serum both had positive correlation with the expression in corresponding tissue (rS=0.826 and 0.889,P<0.001). miR21 in serum of gastric cancer patients reduced observably after operation(t=0.511,P=0.030), while let7a increased (t=0.770, P<0.001). Differences of miR21 and let7a in serum were both statistically significant in 5 groups (χ2=10.841, P=0.028;χ2=26.460, P<0.001). Compared with normal control group, miR21 in serum of patients with gastric cancer increased, while let7a in serum of patients with intestinal metaplasia and gastric cancer decreased. Conclusion:miR21 and let7a in serum may become a new effective biologic marker for noninvasive diagnosing of gastric cancer and the assessment of the results of operations.

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备注/Memo

备注/Memo:
#通讯作者,男,1956年6月生,博士,教授,研究方向:消化道肿瘤,Email:fengchangwei@126.com
更新日期/Last Update: 1900-01-01