[1]郭素平),马军)#,唐芙爱),等.食管鳞状细胞癌组织中Galectin9和MMP2蛋白的表达及与预后的关系*[J].郑州大学学报(医学版),2012,(06):749.
 GUO Suping,MA Jun,TANG Fuai,et al.Relationship between prognosis and expressions of Galectin9 and MMP2 in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(06):749.
点击复制

食管鳞状细胞癌组织中Galectin9和MMP2蛋白的表达及与预后的关系*
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年06期
页码:
749
栏目:
食管癌研究
出版日期:
2012-11-20

文章信息/Info

Title:
Relationship between prognosis and expressions of Galectin9 and MMP2 in esophageal squamous cell carcinoma tissue
作者:
郭素平1)马军2)#唐芙爱1)鲁迪1)段芳龄2)郭琦1)
1)郑州大学第二附属医院消化内科 郑州 450014
2) 郑州大学第二附属医院消化研究所 郑州 450014
Author(s):
GUO Suping1)MA Jun2)TANG Fu′ai1)LU Di1)DUAN Fangling2)GUO Qi1)
1)Department of Gastroenterology,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014
2)Digestive Disease Institute,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014
关键词:
食管肿瘤鳞状细胞癌组织芯片半乳糖凝集素-9基质金属蛋白酶2预后
Keywords:
esophagus neoplasmsquamous cell carcinomatissue microarrayGalectin-9MMP-2prognosis
分类号:
R735.1
摘要:
目的:探讨食管鳞状细胞癌(简称食管鳞癌)组织中Galectin9和MMP2蛋白的表达及与预后的关系。方法:制备组织芯片,应用免疫组化SP法检测63例食管鳞癌及其癌旁正常食管黏膜组织中Galectin9和MMP2蛋白的表达,分析2种蛋白的表达与临床病理指标和预后的关系。结果:食管鳞癌组织中Galectin9蛋白的阳性表达率为62.5%,低于癌旁正常食管黏膜组织的89.3%(χ2=11.529,P<0.001),其表达与肿瘤的分化程度有关(P=0.001),且阳性表达者生存期更长(P=0.013)。食管鳞癌组织中MMP2蛋白的阳性表达率为67.9%,高于癌旁正常食管黏膜组织的357%(χ2=9.633,P=0.001),其表达与肿瘤浸润深度、TNM分期、淋巴结转移有关(P均<005),且阳性表达者生存期较短(P=0.036)。结论:食管鳞癌组织中Galectin9低表达,MMP2高表达;检测二者可用于判断食管鳞癌预后。
Abstract:
Aim:To explore the expressions of Galectin9 and MMP2 proteins,and the relationship between their expressions and prognosis of the patients with esophagus squamous cell carcinoma(ESCC).Methods:The expressions of Galectin9 and MMP2 proteins in 63 cases of ESCC and adjacent nontumor tissue were detected using tissue microarray and immunohistochemical technique.The association of Galectin9 and MMP2 protein expressions with clinicopathologic data and survival were analyzed.Results:The positive expression rate of Galectin9 protein in adjacent nontumor tissue and ESCC tissue were 89.3% and 62.5%,and there was significant difference(χ2=11.529,P<0.001).In ESCC,its expression was related with tumor differentiation(P=0.001).The patients with Galectin9 positive expression had a longer survival period(P=0.013).The positive rate of MMP2 protein in adjacent nontumor tissue and ESCC tissue were 35.7% and 679%,and there was significant difference(χ2=9.633,P=0.001).In ESCC,the expression of MMP2 was related with tumor invasion,lymph node metastasis,and clinical stage,and patients with MMP2 positive expression showed a shorter survival period(P=0036).Conclusion:In ESCC,the Galectin9 protein is downregulated and MMP2 is upregulated.Galectin9 and MMP2 could be markers for prognostic diagnosis of ESCC.

参考文献/References:

[1]Elola MT,WolfensteinTodel C,Troncoso MF,et al.Galectins:matricellular glycanbinding proteins linking cell adhesion,migration,and survival[J].Cell Mol Life Sci,2007,64(13):1679
[2]庞霞,郑湘予,李晟磊.食管鳞癌组织中半乳糖凝集素3和 Bcl2蛋白的表达[J].郑州大学学报:医学版,2010,45(1):8
[3]Dardalhon V,Anderson AC,Karman J,et al.Tim3/galectin9 pathway:regulation of Th1 immunity through promotion of CD11b+Ly6G+ myeloid cells[J].J Immunol,2009,185(3):1383
[4]吕晓智,邹亚光,殷学民,等.MMP1 mRNA在口腔鳞癌与正常黏膜的表达及临床意义[J].南方医科大学学报,2008,28(8):1362
[5]温洪涛,张蕾,张继昌,等.人食管鳞癌组织中MMP2、MMP9和TIMP1、TIMP2的表达[J].郑州大学学报:医学版,2006,41(3):418
[6]陈香丽,王连才,张王刚,等.S100A4和MMP9在非小细胞肺癌中的表达及与浸润、转移和预后的关系[J].南方医科大学学报,2008,28(7):1254
[7]Chabot S,Kashio Y,Seki M,et al.Regulation of galectin9 expression and release in Jurkat T cell line cells[J].Glycobiology,2002,12(2):111
[8]Liang M,Oomizu S,Arikawa T,et al.Galectin9 expression links to malignant potential of cervical squamous cell carcinoma[J].J Cancer Res Clin Oncol,2008,134(8):899
[9]向江东,席晓薇.半乳凝素9与肿瘤[J].现代肿瘤医学,2010,18(5):1014
[10]Kageshita T,Kashi Y,Yamauchi A,et al.Possible role of galectin9 in cell aggregation and apoptosis of human melanoma cell lines and its clinical significance[J].Int J Cancer,2002,99(6):809
[11]Yamauchi A,Kontani K,Kihara M,et al.Galectin9,a novel prognostic factor with antimetastatic potential in breast cancer[J].Breast J,2006,12(5 Suppl 2):S196
[12]Wang X,Lu N,Niu B,et al.Overexpression of auroraA enhances invasion and matrix metalloproteinase2 expression in esophageal squamous cell carcinoma cells[J].Mol Cancer Res,2012,10(5):588
[13]Groblewska M,Siewko M,Mroczko B,et al.The role of matrix metalloproteinases(MMPS) and their inhibitors(TIMPS) in the development of esophageal cancer[J].Folia Histochem Cytobiol,2012,50(1):12

相似文献/References:

[1]张威,陈玉,亚国伟,等.RNA干扰对EC9706细胞mTOR表达及细胞生长与凋亡的影响*[J].郑州大学学报(医学版),2010,(01):11.
 ZHANG Wei,CHEN Yu,YA Guowei,et al.Effect of mTOR siRNA on the expression of mTOR gene,growth and apoptosis of human esophageal carcinoma EC9706 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(06):11.
[2]王彦平),代丽萍),吴晓冰),等.细胞色素P4501A1 Ile/Val位点基因多态性与食管癌关系的meta 分析*[J].郑州大学学报(医学版),2010,(02):186.
 WANG Yanping),DAI Liping),WU Xiaobing),et al.Metaanalysis of cytochrome P4501A1 Ile/Val gene polymorphism with esophageal carcinoma[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(06):186.
[3]陈玉,亚国伟,张威,等.雷帕霉素靶蛋白RNA干扰联合雷帕霉素对人食管癌移植瘤生长和移植瘤组织中雷帕霉素蛋白基因表达的影响*[J].郑州大学学报(医学版),2009,(06):1135.
 CHEN Yu,YA Guowei,ZHANG Wei,et al.Effects of RNAi combined with rapamycin on expression of mTOR gene in tumor tissue of nude mice transplanted with human esophageal cancer cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):1135.
[4].小切口左前外侧开胸食管癌和贲门癌切除术疗效观察*[J].郑州大学学报(医学版),2009,(02):249.
 ZHOU Fuyou),WANG Lidong),SONG Qingrong),et al.Clinical analysis on 552 cases of short surgical incisim via short left anterolater for esophageal and gastric cardia cancer[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):249.
[5]王立东#.内窥镜在食管癌高发区高危人群筛查中的局限和对策[J].郑州大学学报(医学版),2009,(01):11.
 WANG Lidong.Limitation and strategies on endoscopic examination in highrisk subject screening for esophageal cancer in highincidence area[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):11.
[6]常云丽),郑鹏远)#,郭涛),等.家族史阳性食管癌组织中hMSH2和PTEN蛋白的表达*[J].郑州大学学报(医学版),2009,(01):28.
 CHANG Yunli),ZHENG Pengyuan),GUO Tao),et al.Expression of hMSH2 and PTEN in esophageal cancer tissues from the esophageal cancer patients with and without family history[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):28.
[7]申秋),王立东)﹟,常扶保),等.食管癌和贲门癌手术切除标本离体前后及固定后的收缩变化*[J].郑州大学学报(医学版),2009,(01):37.
 SHEN Qiu),WANG Lidong),CHANG Fubao),et al.Effects of specimens shrinkage by surgical resection and fixation on esophageal and gastric cardia cancers[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):37.
[8]张广平)),王立东)﹟,冯常炜),等.食管癌高发区同卵双胞胎食管、贲门和胃窦活检组织病理比较*[J].郑州大学学报(医学版),2009,(01):39.
 ZHANG Guangping),WANG Lidong),FENG Changwei),et al.Histopathological comparison on the biopsies at esophagus, gastric cardia and antrum from monozygotic twins at highincidence area for esophageal cancer[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):39.
[9]周福有)),王立东)﹟,王建坡)),等.食管癌快速康复外科应用效果分析*[J].郑州大学学报(医学版),2009,(01):63.
 ZHOU Fuyou),WANG Lidong),WANG Jianpo),et al.Analysis on fast track surgery after esophagectomy of esophageal cancer[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):63.
[10]蒋蕾,蒋烈夫#,贺建元,等.早期隆起型食管癌X线气钡双对比造影56例体会[J].郑州大学学报(医学版),2009,(01):66.
[11]庞霞,郑湘予,李晟磊,等.食管鳞癌组织中半乳糖凝集素3和Bcl2蛋白的表达*[J].郑州大学学报(医学版),2010,(01):8.
 PANG Xia,ZHENG Xiangyu,LI Shenglei,et al.Expression of Galectin3 and Bcl2 protein in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(06):8.
[12]崔玲玲),李春阳),王旗),等.食管鳞癌组织中EphrinB2、半胱氨酸蛋白酶8蛋白和mRNA的表达*[J].郑州大学学报(医学版),2010,(01):21.
 CUI Lingling),LI Chunyang),WANG Qi),et al.Expression of EphrinB2 and Caspase8 protein and mRNA in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(06):21.
[13]吴欣爱),王筱璨),樊青霞),等.食管鳞状细胞癌组织中乏氧诱导因子1α蛋白和mRNA的表达[J].郑州大学学报(医学版),2010,(02):183.
 WU Xinai),WANG Xiaocan),FAN Qingxia),et al.Expression of HIF1α protein and mRNA in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(06):183.
[14]刘欣欣,赵江沙,印宏坤,等.食管鳞状细胞癌组织中表皮调节素、双调蛋白及表皮生长因子受体mRNA的表达[J].郑州大学学报(医学版),2010,(02):189.
 LIU Xinxin,ZHAO Jiangsha,YIN Hongkun,et al.Expression of epiregulin,amphiregulin,and epidermal growth factor receptor mRNA in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(06):189.
[15]陈妍琼),赵志华),张蕾)#,等.食管鳞状细胞癌组织中KiSS1蛋白和微血管密度的检测*[J].郑州大学学报(医学版),2009,(06):1127.
 CHEN Yanqiong),ZHAO Zhihua),ZHANG Lei),et al.Expressions of KiSS1 protein and detection of MVD in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):1127.
[16]史祖宣,李克,樊青霞#.食管鳞状细胞癌组织中神经性钙黏附蛋白和mRNA的表达*[J].郑州大学学报(医学版),2009,(06):1138.
 SHI Zuxuan,LI Ke,FAN Qingxia.Expression of Ncadherin protein and mRNA in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):1138.
[17]张蕾),贺付成),张云汉)#.食管鳞癌组织中分化相关基因NDRG1的表达及肿瘤诱导分化治疗的研究进展*[J].郑州大学学报(医学版),2009,(05):933.
[18].河南食管癌高发区食管贲门双源癌前病变病理特征*[J].郑州大学学报(医学版),2009,(02):229.
[19]卢红),樊青霞)#,王留兴).Ec9706/P1细胞多药耐药基因1、多药耐药相关蛋白基因1及β微管蛋白Ⅳ基因mRNA的表达[J].郑州大学学报(医学版),2009,(02):236.
 LU Hong),FAN Qingxia),WANG Liuxing).Expression of multidrug resistance gene1, multidrug resistance protein1, and βtubulin Ⅳ mRNA in Ec9706/P1 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):236.
[20]林娜,李克,王鑫,等.食管鳞状细胞癌组织中上皮型和神经型钙黏附蛋白的表达*[J].郑州大学学报(医学版),2009,(02):238.
 LIN Na,LI Ke,WANG Xin,et al.Expression of Ecadherin and Ncadherin in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):238.

备注/Memo

备注/Memo:
*河南省医学科技攻关计划普通项目200903060
#通讯作者,男,1966年8月生,博士,副教授,研究方向:消化道肿瘤的防治、肿瘤细胞免疫治疗,Email:madejun_2@yahoo.com.cn
更新日期/Last Update: 2013-07-03