[1]李杰),梁守沛),陆皓泉),等.肺腺癌组织中HSP70多肽复合物的分离及纯化*[J].郑州大学学报(医学版),2012,(06):753.
 LI Jie,LIANG Shoupei,LU Haoquan,et al.Separation and purification of heat shock protein 70 peptide complex from human lung adenocarcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(06):753.
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肺腺癌组织中HSP70多肽复合物的分离及纯化*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年06期
页码:
753
栏目:
论著
出版日期:
2012-11-20

文章信息/Info

Title:
Separation and purification of heat shock protein 70 peptide complex from human lung adenocarcinoma tissue
作者:
李杰1)梁守沛2)陆皓泉1)孙鹏辉1)王小龙1)许东1)姚武1)周舫1)#
1)郑州大学公共卫生学院劳动卫生学教研室 郑州 450001
2)郑州人民医院医务科 郑州 450003
Author(s):
LI Jie1)LIANG Shoupei2)LU Haoquan1)SUN Penghui1)WANG Xiaolong1)XU Dong1)YAO Wu1)ZHOU Fang1)
1)Department of Occupation Health,College of Public Health,Zhengzhou University,Zhengzhou 450001
2)Department of Medical Administration,Zhengzhou People’s Hospital,Zhengzhou 450003
关键词:
肺腺癌HSP70多肽复合物蛋白分离纯化
Keywords:
lung adenocarcinomaHSP70 peptide complexprotein separation and purification
分类号:
R135.99
摘要:
目的:探讨肺腺癌组织中HSP70多肽复合物分离及纯化的可行性方案。方法:采用裂解液裂解、超声破碎、ConAsepharose亲和层析和DEAE阴离子交换层析方法,从肺腺癌组织中分离纯化HSP70蛋白复合物;通过SDSPAGE电泳、Western blot检测获得蛋白;运用Bradford法对其进行定量分析。结果:通过该实验方法可以从1 g肺癌组织中获取110 μg左右的HSP70多肽复合物,其相对分子质量为70 000。结论:采用ConAsepharose亲和层析和DEAE阴离子亲和层析相结合的蛋白纯化法可以获取高纯度的HSP70多肽复合物。
Abstract:
Aim:To explore a method of separating and purifying HSP70 peptide complex from human lung adenocarcinoma.Methods:HSP70 peptide complex was separated and purified from human lung adenocarcinoma tissue by means of cell lysis,ultrasonication,ConAsepharose column,and DEAE ionexchange chromatogaraphy.The purified fractions were tested by SDSPAGE and Western blot.And quantitative analysis was made by Bradford.Results:About 110 μg HSP70 peptide complex was obtained from 1 g lung adenocarcinoma tissue by this experimental method,with relative molecular weight of 70 000.Conclusion:The high purity HSP70 peptide complex could be obtained by ConAsepharose column and DEAE ionexchange chromatogaraphy.

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目30600490;河南省教育厅科技创新人才支持计划基金资助项目2008HASTIT028;河南省教育厅自然科学研究基金资助项目2008A320018
#通讯作者,女,1974年7月生,博士,副教授,研究方向:职业肿瘤,E-mail:zhoufang23@sina.com
更新日期/Last Update: 2013-07-03