[1]蔡要欣),张韧铮),孙岚),等.纳米活性炭吸附多烯紫杉醇对A549细胞增殖及凋亡的影响*[J].郑州大学学报(医学版),2012,(06):759.
 CAI Yaoxin,ZHANG Renzheng,SUN Lan,et al.Effects of docetaxelloaded activated carbon nanoparticles on proliferation and apoptosis of A549 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(06):759.
点击复制

纳米活性炭吸附多烯紫杉醇对A549细胞增殖及凋亡的影响*
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年06期
页码:
759
栏目:
论著
出版日期:
2012-11-20

文章信息/Info

Title:
Effects of docetaxelloaded activated carbon nanoparticles on proliferation and apoptosis of A549 cells
作者:
蔡要欣12)张韧铮2)孙岚1)宋东颖13)刘贵富4)张莉蓉2)#张英鸽1)#
1)军事医学科学院毒物药物研究所 北京 100850
2)郑州大学基础医学院药理学教研室 郑州 450001
3)吉林大学药学院 长春 130021
4)中国人民解放军66018部队卫生队 北京 100144
Author(s):
CAI Yaoxin12)ZHANG Renzheng2)SUN Lan1)SONG Dongying13)LIU Guifu4)ZHANG Lirong2)ZHANG Yingge1)
1)Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850

2)Department of Pharmacology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001

3)College of Pharmacy,Jilin University,Changchun 130021

4)Chinese People’s Liberation Army 66018 Military Health Team,Beijing 100144
关键词:
纳米活性炭多烯紫杉醇A549细胞凋亡增殖
Keywords:
activated carbon nanoparticledocetaxelA549 cellapoptosisproliferation
分类号:
R734.2
摘要:
目的:研究纳米活性炭吸附多烯紫杉醇(ACNPDOC)对人肺腺癌细胞A549增殖及凋亡的影响。方法:将A549细胞分别用0.2、1.0、5.0、25.0和125.0 μmol/L DOC和ACNPDOC培养12、24、48和72 h后,MTT法测细胞增殖抑制率。将A549分别用IC50浓度的ACNPDOC、DOC和与ACNPDOC等量的ACNP培养24 h后,应用流式细胞仪检测细胞凋亡率。结果:0.2~125.0 μmol/L的DOC及ACNPDOC对A549细胞均有增殖抑制作用,作用呈浓度和时间依赖性,ACNPDOC组细胞增殖抑制率高于DOC组(F药物=12.326,F时间=76.247,F浓度=53.028,F交互=20.842,P<0.05)。ACNPDOC的IC50为0.80 μmol/L,DOC为43.45 μmol/L。ACNP、DOC和ACNPDOC组的细胞总凋亡率差异有统计学意义(F=30.208,P<0.001),ACNPDOC组凋亡率高于ACNP组和DOC组(P均<0001)。结论:ACNPDOC较DOC对A549细胞有较强的增殖抑制及诱导凋亡作用。
Abstract:
Aim:To study the effects of docetaxel(DOC)loaded activated carbon nanoparticles(ACNPDOC) on proliferation and apoptosis of human lung cancer A549 cells.Methods:A549 cells were teated with 0.2,1.0,5.0,25.0 and 125.0 μmol/L DOC or ACNPDOC for 12,24,48 and 72 h,respectively,then the proliferation inhibition rate was assayed by MTT.A549 cells were teated with ACNPDOC,DOC and ACNP at dose of IC50 for 24 h,then the apoptosis rate was detected by flow cytometry.Results:DOC and ACNPDOC in the concentration range of 0.2~125.0 μmol/L could inhibit A549 cell proliferation in a time and concentrationdependent manner,but the effects of ACNPDOC were significantly stronger(Fdrug=12.326,Ftime=76.247,Fdose=53.028,Finteraction=20.842,P<0.05).IC50 of ACNPDOC and DOC was 080 and 43.45 μmol/L,respectively.The apoptosis rate of A549 cells treated with ACNP,DOC,or ACNPDOC at the dose of IC50 differed from each other(F=30.208,P<0.001),and that of ACNPDOC group was higher than those of ACNP group and DOC group(P<0.001).Conclusion:ACNPDOC can inhibit A549 cell proliferation,induce apoptosis,and the effects is stronger than DOC.

参考文献/References:

[1] 张阳德.纳米生物技术学[M].北京:科学出版社,2005:1

[2]孙岚,张英鸽.小鼠腹腔注射纳米活性炭体内去向追踪观察[J].中国药理学与毒理学杂志,2003,17(4):316

[3]曲秋莲,张英鸽.胃癌靶向缓释制剂——活性炭吸附抗癌药研究进展[J].中华医学实践杂志,2008,7(5):399

[4]杨钟,赵君贤,李培锋,等.活性炭纳米粒子对5Fu抗肿瘤作用的影响[J].军事医学科学院院刊,2009,33(5):416

[5]张浩,孙岚,鄂晓,等.纳米活性炭吸附丝裂霉素C腹腔化疗的生物发光成像研究[J].军事医学,2011,35(4):299

[6]黄广建,陈忠清,刘铭,等.活性炭纳米粒对胃癌淋巴清扫的指导作用[J].中国现代医学杂志,2005,15(2):233

[7]陈浩,黄广建,倪泉兴,等.纳米活性碳作为药物载体在淋巴靶向治疗中的作用[J].复旦学报:医学版,2007,34(4):518

[8]Stearns RC,Paulauskis JD,Godleski JJ.Endocytosis of ultrafine particles by A549 cells[J].Am J Respir Cell Mol Biol,2001,24(2):108

[9]Bitterle E,Karg E,Schroeppel A,et al.Dosecontrolled exposure of A549 epithelial cells at the airliquid interface to airborne ultrafine carbonaceous particles[J].Chemosphere,2006,65(10):1784

[10]Sayes CM,Wahi R,Kurian PA,et al.Correlating nanoscale titania structure with toxicity:a cytotoxicity and inflammatory response study with human dermal fibroblasts and human lung epithelial cells[J].Toxicol Sci,2006,92(1):174

[11]Viktorsson K,Lewensohn R,Zhivotovsky B,et al.Apoptotic pathways and therapy resistance in human malignancies[J].Adv Cancer Res,2005,94:143

[12]Tsuruo T,Naito M,Tomida A,et al.Molecular targeting therapy of cancer:drug resistance,apoptosis and survival signal[J].Cancer Sci,2003,94(1):15

[13]王岳勇,陈志涛,何勇.肾细胞癌中survivin基因的表达及其与细胞凋亡的关系[J].实用医学杂志,2006,22(5):489

备注/Memo

备注/Memo:
*国家863计划项目2006AA03Z333;国家973计划项目2010CD933904
#通讯作者,张英鸽,男,1958年10月生,博士,教授,研究方向:纳米药理,E-mail:zhangyg58@126.com;张莉蓉,女,1964年10月生,博士,教授,研究方向:遗传药理,E-mail:lrzhang@zzu.edu.cn
更新日期/Last Update: 2013-07-03