[1]陆永光△,符春晖,严华,等.阿托伐他汀对内皮细胞微粒诱导的人脐静脉内皮细胞ERK、p38MAPK、NFκB p65蛋白及ICAM1 mRNA 表达的影响*[J].郑州大学学报(医学版),2012,(06):765.
 LU Yongguang,FU Chunhui,YAN Hua,et al.Effects of atorvastatin on expressions of ERK,p38MAPK,NFκB proteins and ICAM1 mRNA in HUVECs induced by endothelial microparticles[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(06):765.
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阿托伐他汀对内皮细胞微粒诱导的人脐静脉内皮细胞ERK、p38MAPK、NFκB p65蛋白及ICAM1 mRNA 表达的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年06期
页码:
765
栏目:
论著
出版日期:
2012-11-20

文章信息/Info

Title:
Effects of atorvastatin on expressions of ERK,p38MAPK,NFκB proteins and ICAM1 mRNA in HUVECs induced by endothelial microparticles
作者:
陆永光符春晖严华陈湘桂黄军章陈丽媛
钦州市第二人民医院心内科 钦州 535099
Author(s):
LU YongguangFU ChunhuiYAN HuaCHEN XiangguiHUANG JunzhangCHEN Liyuan
Department of Cardiology,the Second People’s Hospital of Qinzhou,Qinzhou 535099
关键词:
内皮细胞微粒脐静脉内皮细胞丝裂素活化蛋白激酶细胞间黏附分子1阿托伐他汀
Keywords:
endothelial microparticlehuman umbilical vein endothelial cellmitogenactivated protein kinaseintercellular adhesion molecule 1atorvastatin
分类号:
R543.3
摘要:
目的:探讨阿托伐他汀干预对内皮细胞微粒(EMPs)诱导的人脐静脉内皮细胞(HUVEC)ERK/MAPK和NFκB信号通路及细胞间黏附分子1(ICAM1)表达的影响。方法:将体外培养的HUVEC细胞系ECV304分组培养:①EMPs不同作用时间组用EMPs (终浓度105 mL-1)分别刺激细胞0、3、6、12和24 h。②EMPs不同作用剂量组分别用终浓度为0、102、103、104及105 mL-1的EMPs刺激细胞24 h。③抑制剂预处理组在EMPs刺激前,分别用ERK、p38MAPK及NFκB抑制剂PD98059、SB203580、PDTC进行预处理。④阿托伐他汀预处理组在EMPs刺激前,用阿托伐他汀进行预处理。然后,用实时荧光定量PCR测定细胞中ICAM1 mRNA的表达,Western blot法测定磷酸化ERK(pERK)、pp38MAPK、NFκB p65蛋白的表达。结果:随EMPs作用时间的延长和作用剂量的增加,细胞pERK、pp38MAPK、NFκB p65蛋白及ICAM1 mRNA的表达均逐渐增加(P均<0.001)。用上述抑制剂及阿托伐他汀预处理后,EMPs诱导的细胞 ICAM1 mRNA表达均降低(P<0.05)。结论:阿托伐他汀可能通过ERK/MAPK和NFκB信号通路下调EMPs诱导的内皮细胞 ICAM1的表达。
Abstract:
Aim:To observe the effects of atorvastatin on ERK,p38MAPK and nuclear factorκB (NFκB) and intercellular adhesion molecule 1(ICAM1) expressions in endothelial microparticles(EMPs)induced human umbilical vein endothelial cells(HUVECs).Methods:The cultured HUVECs were cultured with EMPs of 105 mL-1for 0,3,6,12,and 24 h.The HUVECs were cultured with 0,102,103,104,105 mL-1 EMPs for 24 h.The HUVECs were pretreated with ERK inhibitor (PD98059),p38 inhibitor (SB203580),NFκB inhibitor (PDTC),or atorvastatin,then were treated with EMPs.The ICAM1 mRNA level was detected by RealtimePCR.Western blot was performed to determine the expression levels of phosphoERK(pERK),pp38MAPK and NFκB p65 proteins.Results:With the increase of dose and exposure time of EMPs,the expression levels of ICAM1 mRNA,pERK,pp38MAPK and NFκB p65 protein in HUVECs obviously increased(P<0.001).After the pretreatment of the inhibitors mentioned above and atorvastatin,the ICAM1 mRNA expression decreased(P<0.05).Conclusion:Atorvastatin could downregulate ICAM1 expression in EMPsinduced HUVECs by blocking ERK/MAPK and NFκB signaling pathway

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备注/Memo

备注/Memo:
*广西自然科学基金青年基金资助项目2012GXNSFBA053113;广西卫生厅自筹经费科研项目 Z2011103;△男,1977年10月生,博士,副主任医师,研究方向:冠心病的诊治,Email:evershine_lu@hotmail.com
更新日期/Last Update: 2013-07-03