[1]韩艳林,翟明霞,吴亚红,等.肿瘤转移相关基因1 HLAA3限制性细胞毒性T淋巴细胞表位的预测与鉴定[J].郑州大学学报(医学版),2012,(06):773.
 HAN Yanlin,ZHAI Mingxia,WU Yahong,et al.Identification of HLAA3 restricted cytotoxic T lymphocyte epitopes from metastasis associated gene 1[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(06):773.
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肿瘤转移相关基因1 HLAA3限制性细胞毒性T淋巴细胞表位的预测与鉴定
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年06期
页码:
773
栏目:
论著
出版日期:
2012-11-20

文章信息/Info

Title:
Identification of HLAA3 restricted cytotoxic T lymphocyte epitopes from metastasis associated gene 1
作者:
韩艳林翟明霞吴亚红高艳锋祁元明#
郑州大学生物工程系 郑州 450001
Author(s):
HAN YanlinZHAI MingxiaWU YahongGAO YanfengQI Yuanming
Department of Bioengineering,Zhengzhou University,Zhengzhou 450001
关键词:
肿瘤转移相关基因1HLAA3细胞毒性T淋巴细胞
Keywords:
metastasis associated gene 1HLAA3cytotoxic T lymphocyte
分类号:
Q279
摘要:
目的:鉴定肿瘤转移相关基因1(MTA1)的HLAA3限制性细胞毒性T淋巴细胞(CTL)表位。方法:首先运用RTPCR方法检测MTA1 mRNA在肿瘤细胞系EC1、EC109和T47D中的表达情况。然后通过BIMAS、SYFPEITHI、NetCTL 1.2及IEDB 4个软件预测筛选MTA1 HLAA3限制性的候选表位。候选表位通过标准Fmoc化学法合成,通过结合力实验检测表位与T2A3细胞表面HLAA3分子的结合力水平,通过体外细胞毒实验检测候选肽诱导CTL的能力。结果:MTA1 mRNA在EC1、EC109和T47D细胞中均有表达。候选表位P130与HLAA3分子有弱结合力,P294、P559与HLAA3分子有中等结合力。细胞毒实验结果显示多肽P130、P294和P559对EC1细胞均有一定的杀伤作用(F细胞=176.107,F效靶比=48.306,F交互=35.686,P均<0.001)。结论:多肽P130、P294、P559能够诱导体外抗肿瘤免疫反应,可能成为新的抗肿瘤多肽疫苗的候选表位。
Abstract:
Aim:To identify the HLAA3 restricted cytotoxic T lymphocyte(CTL) epitopes from metastasis associated gene 1(MTA1).Methods:RTPCR was used to determine the expression of MTA1 mRNA in cancer cell lines,EC1,EC109 and T47D.HLAA3 epitopes from MTA1 protein were predicted by BIMAS,SYFPEITHI,NetCTL 1.2 and IEDB.Peptides were synthesized by standard Fmoc chemistry method.Their binding affinities towards HLAA3 molecule were evaluated by T2A3 cells binding assay.Their ability to induce T cell response was investigated by using cytotoxicity assay in vitro.Results:The expression of MTA1 mRNA was observed in EC1,EC109 and T47D cells.The candidate peptide P130 showed weak affinity towards HLAA3 molecule,while P294 and P559 showed moderate affinity.The CTLs induced by all the three peptides could lyse EC1 cells(Fcell=176.107,Fratio=48.306,Finteraction=35.686,P<0.001).Conclusion:The peptides P130,P294,and P559 could induce antitumor inmmunity in vitro and serve as candidates towards antitumor peptide vaccines.

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目81172893,30901362;郑州大学研究生科学研究基金资助项目11L00403;
#通讯作者,女,1957年1月生,博士,教授,研究方向:抗原肽和多肽疫苗,Email:qym@zzu.edu.cn
更新日期/Last Update: 2013-07-03