[1]王瑞,张杰,张卫星#,等.黄芪多糖对大剂量60Co γ射线损伤大鼠睾丸间质细胞的影响*[J].郑州大学学报(医学版),2012,(06):791.
 WANG Rui,ZHANG Jie,ZHANG Weixing,et al.Effects of astragalus polysacharin on rat leydig cells exposed to 60Co γ ray[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(06):791.
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黄芪多糖对大剂量60Co γ射线损伤大鼠睾丸间质细胞的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年06期
页码:
791
栏目:
论著
出版日期:
2012-11-20

文章信息/Info

Title:
Effects of astragalus polysacharin on rat leydig cells exposed to 60Co γ ray
作者:
王瑞张杰张卫星#秦俊昌王磊
郑州大学第一附属医院泌尿外科;河南省高等学校临床医学重点学科开放实验室;郑州大学肿瘤分子外科研究所 郑州 450052
Author(s):
WANG RuiZHANG JieZHANG WeixingQIN JunchangWANG Lei
Department of Urology,the First Affiliated Hospital,Zhengzhou University;Open Laboratory of Key Discipline of Clinical Medical Sciences,Higher School of Henan Province;the Institute of Molecular Cancer Surgery of Zhengzhou University,Zhengzhou 450052
关键词:
黄芪多糖睾丸间质细胞60Co γ射线大鼠
Keywords:
astragalus polysacharinleydig cell60Co γ rayrat
分类号:
R285.5;R697.22
摘要:
目的:探讨黄芪多糖(APS)对60Co γ射线损伤大鼠睾丸间质细胞的防治作用。方法:雄性SD大鼠30只,随机分为空白对照组、60Co γ照射组、APS+60Co γ照射组。观察各组大鼠睾丸间质细胞的形态学改变;ELISA法检测血清睾酮(T)、黄体生成素(LH)的含量;RTPCR法检测睾丸间质细胞黄体生成素受体(LHR)mRNA水平。结果:与空白对照组相比,60Co γ照射组大鼠睾丸间质细胞形态发生改变,细胞体积缩小,胞质减少,细胞核皱缩,数目减少;APS+60Co γ照射组睾丸组织病理改变较60Co γ照射组减轻。3组大鼠血清 T及LH水平差异无统计学意义(F=2.446和1.142,P>0.05),睾丸组织中LHR的mRNA的表达差异有统计学意义(F=24.032,P<0.001)。结论:APS可防治60Co γ射线对睾丸间质细胞损伤,其机制可能与促进睾丸间质细胞LHR mRNA的高表达有关。
Abstract:
Aim:To investigate the protection of astragalus polysacharin(APS) on the impairment of the leydig cells caused by higher doses of 60Co γ in rats.Methods:Thirty male SD rats were randomized into three groups(n=10):the normal control group,the radiation control group,the APS plus radiation group.The structural changes of the leydig cells were observed with light microscope.The serum luteinizing hormone(LH) and testosterone(T) levels were measured by ELISA;and the mRNA expression level of luteinizing hormone receptor(LHR) from the leydig cells surface was measured by RTPCR.Results:Compared with the normal control group,the leydig cells exhibited decreases in size,cytoplasm,and quantity as well as the nuclear shrinkage after radiation.The mRNA expression levels of LHR were lower(P<0.01);in the APS irrigation group,the pathological changes of leydig cells were lightly.The mRNA expression levels were close to the normal;the serum LH and T levels were no significant change.Conclusion:APS can reduce the impairment of the leydig cells caused by the higher doses 60Co γ ray in rats,which may be involved in its induce the high expression of LHR mRNA.

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备注/Memo

备注/Memo:
*河南省科技攻关基金资助项目102102310067;
#通讯作者,男,1957年7月生,博士,教授,主任医师,研究方向:泌尿外科、男科学,Email:huzi918@hotmail.com
更新日期/Last Update: 2013-07-03