[1]王文佳,张正全,贾欣#,等.2甲氧基雌二醇对4T1、SPCA1和PC3细胞的生长抑制作用[J].郑州大学学报(医学版),2012,(06):804.
 WANG Wenjia,ZHANG Zhengquan,JIA Xin,et al.Growth inhibition effects of 2methoxyestradiol on 4T1,SPCA1 and PC3 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(06):804.
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2甲氧基雌二醇对4T1、SPCA1和PC3细胞的生长抑制作用
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年06期
页码:
804
栏目:
论著
出版日期:
2012-11-20

文章信息/Info

Title:
Growth inhibition effects of 2methoxyestradiol on 4T1,SPCA1 and PC3 cells
作者:
王文佳张正全贾欣#李雪冰张振中
郑州大学药学院 郑州 450001
Author(s):
WANG WenjiaZHANG ZhengquanJIA XinLI XuebingZHANG Zhenzhong
School of Pharmaceutical Sciences,Zhengzhou University,Zhengzhou 450001
关键词:
2甲氧基雌二醇维拉帕米协同指数4T1SPCA1细胞PC3细胞
Keywords:
2methoxyestradiolverapamilsynergic index4T1 cellSPCA1 cellPC3 cell
分类号:
R979.1
摘要:
目的:探讨2甲氧基雌二醇(2ME)对多种肿瘤细胞的生长抑制作用。方法:以鼠乳癌4T1细胞株、人肺腺癌SPCA1细胞株、人前列腺癌PC3细胞株为研究对象,采用SRB法考察2ME对多种肿瘤细胞的生长抑制作用,并联合维拉帕米,利用协同指数探讨影响2ME抗癌作用的因素。结果:2ME对4T1、SPCA1和PC3细胞有不同程度的抑制作用,IC50分别是6.11、1.89和5.12 μmol/L;联合维拉帕米后,抑制率高于单独用药组(P<001),IC50分别降低至2.01、0.57和2.77 μmol/L,协同指数0.77~0.43。结论:2ME对3种细胞都有一定的生长抑制作用,维拉帕米对2ME有明显的增效作用。
Abstract:
Aim:To explore growth inhibition effect of 2methoxyestradiol(2ME) on a variety of tumor cells.Methods:The study object was 4T1 cell strain of rat mastocarcinoma,SPCA1 cell strain of human pulmonary adenocarcinoma and PC3 cell strain of human prostate cancer.SRB method was adopted to investigate growth inhibition effect of 2ME on the above tumor cells.In addition,verapamil and synergic index were used to explore the factors influencing 2ME anticancer effect.Results:2ME had inhibition effects on 4T1,SPCA1 and PC3 cells with IC50 of 6.11,1.89 and 5.12 μmol/L,respectively.After verapamil was adopted,the inhibitory rate was significantly higher than that of the singledrug group(P<0.01),and IC50 dropped to 2.01,0.57 and 2.77 μmol/L,respectively,with the synergic index distributed within 077~0.43.Conclusion:2ME has inhibition effects on the three kinds of tumor cells,and verapamil has obvious synergistic effect on 2ME.

参考文献/References:

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[1]贾欣),韩淑萍),孙惠斌),等.肺癌荷瘤小鼠体内组织中2甲氧基雌二醇纳米混悬剂的分布*[J].郑州大学学报(医学版),2014,(06):792.
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备注/Memo

备注/Memo:

#通讯作者,女,1975年8月生,硕士,讲师,研究方向:肿瘤靶向药物治疗,Email:jiaxin@zzu.edu.cn
更新日期/Last Update: 2013-07-03