[1]张莎,袁君,张贵星,等.表没食子儿茶素没食子酸酯对A549细胞增殖、凋亡和hTERT表达的影响[J].郑州大学学报(医学版),2012,(06):807.
 ZHANG Sha,YUAN Jun,ZHANG Guixing,et al.Effects of epigallocatechin3gallate on growth and hTERT expression of A549 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(06):807.
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表没食子儿茶素没食子酸酯对A549细胞增殖、凋亡和hTERT表达的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年06期
页码:
807
栏目:
应用研究
出版日期:
2012-11-20

文章信息/Info

Title:
Effects of epigallocatechin3gallate on growth and hTERT expression of A549 cells
作者:
张莎袁君张贵星张菊娥#
郑州大学基础医学院生物化学与分子生物学教研室 郑州 450001
Author(s):
ZHANG ShaYUAN JunZHANG GuixingZHANG Ju’e
Department of Biochemistry and Molecular Biology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001
关键词:
表没食子儿茶素没食子酸酯人端粒酶逆转录酶肺肿瘤A549细胞
Keywords:
epigallocatechin3gallatehuman telomerase reverse transcriptaselung neoplasmA549 cell
分类号:
R734.2
摘要:
目的:探讨表没食子儿茶素没食子酸酯(EGCG)对人肺腺癌细胞株A549细胞增殖、凋亡及人端粒酶逆转录酶(hTERT)表达的影响。方法:用0、50、100、150、200、250 mg/L EGCG分别作用 A549细胞 24、48 和72 h后,MTT比色法检测细胞增殖抑制率;0、100、200、250 mg/L EGCG干预48 h后,应用Annexin VFITC/PI双染法检测A549细胞的凋亡情况,应用RTPCR和免疫细胞化学分别检测细胞内hTERT mRNA和蛋白的表达。结果:EGCG可抑制A549细胞的增殖,并随着剂量增加和时间延长,抑制率增加(F组间=185.944,F时间=291.234,F交互=4 186119,P均<0.001)。EGCG促进 A549细胞凋亡(F=2 757.183,P<0.001),还可抑制 A549细胞中hTERT mRNA与蛋白的表达(F=260.861和953.327,P均<0.001)。结论:EGCG能有效地抑制 A549细胞的增殖,诱导其凋亡,其机制可能与下调 hTERT 的表达有关。
Abstract:
Aim:To investigate the effects of epigallocatechin3gallate(EGCG)on proliferation and apoptosis and the expression of human telomerase reverse transcriptase(hTERT)of human lung adenocarcinoma cell line A549.Methods:A549 cells were treated with 0,50,100,150,200,250 mg/L EGCG for 24,48,and 72 h,respectively.MTT assay was used to detect the inhibitory rate of cell proliferation.Flow cytometry with Annexin VFITC/PI double staining was employed to analyze the apoptosis of A549 cells treated with various concentrations of EGCG(0,100,200,250 mg/L)for 48 h,and RTPCR and immunocytochemistry were applied to detect the mRNA and protein expression of hTERT.Results:EGCG inhibited the cell proliferation of A549 in a dose dependent manner(Fgroup=185.944,Ftime=291.234,Finteraction=4 186.119,P<0.001),and promoted the apoptosis of A549 cells(F=2 757.183,P<0.001).EGCG could suppress the mRNA and protein expression of hTERT(F=260.861 and 953.327,P<0.001).Conclusion:EGCG can effectively inhibit cell proliferation and induce apoptosis of A549 cells,which may be associated with downregulation of hTERT expression.

参考文献/References:

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[8]池花明,陶泽璋,陈始明,等.抑制hTERT表达对喉癌细胞凋亡及其相关蛋白的影响[J].中国耳鼻咽喉颅底外科杂志,2006,12(1):5

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备注/Memo

备注/Memo:

#通讯作者,女,1953年10月生,硕士,教授,研究方向:肿瘤酶学,Email:Zje8866@126.com
更新日期/Last Update: 2013-07-03