[1]单磊,郝建伟#,王向阳.LNCaP冻融抗原致敏的树突状细胞诱导的CTL对LNCaP细胞的杀伤作用*[J].郑州大学学报(医学版),2012,(06):824.
 SHAN Lei,HAO Jianwei,WANG Xiangyang.Effects of cytotoxicity T lymphocyte activated by dendritic cell loaded with LNCaP cell line frozenthawing antigen on LNCaP cell[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(06):824.
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LNCaP冻融抗原致敏的树突状细胞诱导的CTL对LNCaP细胞的杀伤作用*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年06期
页码:
824
栏目:
应用研究
出版日期:
2012-11-20

文章信息/Info

Title:
Effects of cytotoxicity T lymphocyte activated by dendritic cell loaded with LNCaP cell line frozenthawing antigen on LNCaP cell
作者:
单磊郝建伟#王向阳
河南省人民医院泌尿外科 郑州 450003
Author(s):
SHAN LeiHAO JianweiWANG Xiangyang
Department of Urology,Henan Provincial People’s Hospital,Zhengzhou 450003
关键词:
树突状细胞前列腺癌免疫治疗
Keywords:
dendritic cellprostate cancerimmunotherapy
分类号:
R737.25
摘要:
目的:研究负载LNCaP冻融抗原的树突状细胞(DC)诱导的细胞毒性T淋巴细胞(CTL)对前列腺癌LNCaP细胞的杀伤效应。方法:采用反复冻融法获得LNCaP细胞抗原,联合应用rhIL4、rhGMCSF和TNFα体外诱导人外周血单个核细胞为成熟DC,并用LNCaP细胞冻融抗原致敏,用该致敏DC体外诱导CTL,观察CTL对LNCaP细胞的特异性杀伤作用,并采用ELISA法检测致敏DC IL12 p70的分泌量。以未致敏DC为对照。结果:冻融抗原致敏DC诱导的CTL对LNCaP细胞具较高杀伤率,该作用强于未致敏DC,且当效靶比为201时效用最显著(F组别=68.314,F效靶比=7.251,F交互=56.233,P<0.05);与未致敏DC相比,致敏DC IL12 p70分泌量显著增加(t=4.020,P=0.002)。结论:LNCaP冻融抗原致敏DC激活的CTL 在体外具有更强的杀伤LNCaP细胞的作用。
Abstract:
Aim:To investigate the immune responses of cytotoxic T lymphocytes(CTL) against prostate cancer cell LNCaP after activated by dendritic cell(DC) loaded with LNCaP frozenthawing antigen in vitro.Methods:LNCaP cells were frozen and melt repeatedly 3 times,then tumor lysate was obtained.After separated from heparinized venous blood of healthy donors by FicollHypaque densitygradient centrifugation,peripheral blood mononuclear cells(PBMCs) were cultured and induced by rhIL4 and rhGMCSF and TNFα to differentiate into DC.The cytotoxicity of CTL activated by DCs loaded with frozenthawing antigen and cytokine release were measured by MTT assay and ELISA.Results:DCs could be harvested through healthy donors’ PBMCs cultured in vitro.CTL activated by lysateloaded DCs had more evident cytotoxicity against LNCaP cells in vitro(Fgroup=68.314,Fratio=7.251,Finteraction=56.233,P<0.05).The level of DCs excreting IL12,which sensitized by LNCaP lysate,was higher than that of unsensitized DCs(t=4.020,P=0.002).Conclusion:CTL activated by DCs loaded with LNCaP frozenthawing antigen exerts a remarkable killing activity on LNCaP cell in vitro.

参考文献/References:


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备注/Memo

备注/Memo:
*河南省卫生厅科技攻关基金资助项目200903134;
#通讯作者,男,1974年7月生,博士,主治医师,研究方向:泌尿系肿瘤,Email:exwhao@126.com
更新日期/Last Update: 2013-07-03