[1]画宝勇)#,马丽华),李时恩).重铬酸钾对A549细胞MSH2 mRNA及蛋白表达的影响[J].郑州大学学报(医学版),2012,(06):841.
 HUA Baoyong,MA Lihua,LI Shien.Effects of potassium bichromate on expression of MSH2 mRNA and protein in A549 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(06):841.
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重铬酸钾对A549细胞MSH2 mRNA及蛋白表达的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年06期
页码:
841
栏目:
应用研究
出版日期:
2012-11-20

文章信息/Info

Title:
Effects of potassium bichromate on expression of MSH2 mRNA and protein in A549 cells
作者:
画宝勇1)#马丽华2)李时恩1)
1)郑州大学公共卫生学院劳动卫生学教研室 郑州 450001
2)郑州大学口腔医学院 郑州 450052
Author(s):
HUA Baoyong1)MA Lihua2)LI Shi’en1)
1)Department of Occupational Health,College of Public Health,Zhengzhou University,Zhengzhou 450001;
2)College of Stomatology,Zhengzhou University,Zhengzhou 450052
关键词:
重铬酸钾MSH2基因错配修复基因A549细胞
Keywords:
potassium bichromateMSH2mismatch repair geneA549 cell
分类号:
R135.1
摘要:
目的:研究重铬酸钾(K2Cr2O7)对A549细胞MSH2 mRNA及蛋白表达的影响。方法:体外培养条件下用0、1.25×10-6、2.50×10-6及5.00×10-6 μmol/L的K2Cr2O7溶液染毒A549细胞24 h后,分别用MTT法、Realtime PCR及Western blot方法检测细胞活性、MSH2 mRNA及蛋白的表达。结果:随K2Cr2O7作用浓度的升高,A549细胞活性、MSH2 mRNA相对表达量和蛋白表达量均逐渐降低(F=175.040、66.128和33.326,P<0001)。结论:K2Cr2O7影响A549细胞的损伤修复。
Abstract:
Aim:To observe the effect of potassium bichromate on the expression of MSH2 mRNA and protein in A549 cells.Methods:A549 cells were treated with 0,1.25×10-6,2.50×10-6 and 5.00×10-6 μmol/L potassium bichromate for 24 h,then cells survival ability,the expressions of MSH2 mRNA and protein were detected by MTT,Real time PCR and Western blot methods,respectively.Results:Cells survival ability,the MSH2 mRNA and protein expression in A549 cells decreased with the potassium bichromate concentration increasing(F=175.040,66.128 and 33.326,P<0001).Conclusion:Potassium bichromate can affect the ability of damage repair of A549 cells.

参考文献/References:

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[2]李瑛,许建宁.001 DNA错配修复基因 hMSH2 与肿瘤关联的研究进展[J].国外医学:卫生学分册,2008,35(1):1

[3]Gomez RL,Galles C,Spampinato CP.Highlevel production of MSH2 from Arabidopsis thaliana:a DNA mismatch repair system key subunit[J].Mol Biotechnol,2011,47(2):120

[4]Li T,Suo Q,He D,et al.Esophageal cancer risk is associated with polymorphisms of DNA repair genes MSH2 and WRN in Chinese population[J].J Thora Oncol,2012,7(2):448

[5]Thompson CM,Haws LC,Harris MA,et al.Application of the US EPA mode of action Framework for purposes of guiding future research:a case study involving the oral carcinogenicity of hexavalent chromium[J].Toxicol Sci,2011,119(1):20

[6]Zhitkovich A.Chromium in drinking water:sources,metabolism and cancer risks[J].Chem Res Toxicol,2011,24(10):1617

[7]Kouso H,Yoshino I,Miura N,et al.Expression of mismatch repair proteins,hMLH1/hMSH2,in nonsmall cell lung cancer tissues and its clinical significance[J].J Surg Oncol,2008,98(5):377

[8]Xinarianos G,Liloglou T,Prime W,et al.hMLH1 and hMSH2 expression correlates with allelic imbalance on chromosome 3p in nonsmall cell lung carcinomas[J].Cancer Res,2000,60(15):4216

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备注/Memo

备注/Memo:

#通讯作者,男,1975年11月生,硕士,副教授,研究方向:公共卫生与预防医学,Email:hby@zzu.edu.cn
更新日期/Last Update: 2013-07-03