[1]祝淮阳△,熊瑜.术前热疗联合灌注化疗对非小细胞肺癌患者T淋巴细胞亚群及血清P53抗体水平的影响[J].郑州大学学报(医学版),2012,(06):844.
 ZHU Huaiyang,XIONG Yu.Effects of preoperative infusion chemotherapy combined with hyperthermia on T lymphocyte subsets and serum P53 antibody level of nonsmall cell lung cancer patients[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(06):844.
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术前热疗联合灌注化疗对非小细胞肺癌患者T淋巴细胞亚群及血清P53抗体水平的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年06期
页码:
844
栏目:
应用研究
出版日期:
2012-11-20

文章信息/Info

Title:
Effects of preoperative infusion chemotherapy combined with hyperthermia on T lymphocyte subsets and serum P53 antibody level of nonsmall cell lung cancer patients
作者:
祝淮阳熊瑜
山东省胸科医院肿瘤综合科 济南 250013
Author(s):
ZHU HuaiyangXIONG Yu
Department of Comprehensive Oncology,Shandong Chest Hospital,Jinan 250013
关键词:
灌注化疗热疗非小细胞肺癌免疫功能
Keywords:
infusion chemotherapyhyperthermianonsmall cell lung cancerimmunity
分类号:
R734.1
摘要:
目的:评估术前热疗联合灌注化疗对非小细胞肺癌患者免疫功能的影响。方法:选取中晚期非小细胞肺癌患者68例,31例(A组)术前给予单纯顺铂和丝裂霉素动脉灌注化疗;余37例(B组)灌注化疗前1 h和注药24 h后,用体外高频热疗机热疗1次。2组分别于灌注化疗前、术前1 天、术后第10天采集静脉血,应用间接免疫荧光法测定T淋巴细胞亚群;分别于灌注化疗前,术前1天,术后第10、30、60和90天抽取清晨空腹静脉血,应用ELISA法检测P53抗体水平。结果:A组术前CD3、CD4水平较灌注化疗前降低,CD8、CD4/CD8轻微升高;术后CD3、CD4及CD4/CD8水平升高,CD8降低。B组术前CD3、CD4水平较灌注化疗前明显增高,CD8降低,CD4/CD8升高;术后上述指标变化趋势未发生改变(P均<0.001)。2组术后血清P53抗体水平均逐渐降低,B组较A组降低明显(F组间=52.735,F时间=9.297,F交互=4.219,P<0.001)。结论:术前热疗联合灌注化疗可以改善晚期非小细胞肺癌患者的免疫力。
Abstract:
Aim:To study the effect of preoperative infusion chemotherapy combined with hyperthermia on the immunity of nonsmall cell lung cancer(NSCLC) patients.Methods:A total of 68 patients with intermediate and advanced NSCLC were divided into two groups.Group A(n=31) was treated with interventional arterial infusion chemotherapy alone.Group B(n=37) was treated with transcatheter arterial infusion chemotherapy combined with hyperthermia.The levels of the subsets of the peripheral blood T lymphocytes(CD3,CD4,CD8)and the P53 antibody were detected by indirect immunofluorescence technique and ELISA,respectively.Results:The CD3 and CD4 levels before operation were significantly lower than those before infusion chemotherapy in group A,CD8 and CD4/CD8 were higher;but CD3,CD4 level and CD4/CD8 increased,and CD8 level decreased after operation.As to group B,CD3,CD4 and CD4/CD8 levels before operation were significantly higher than those before infusion chemotherapy,CD8 were lower;and the trend didn’t change after operation when compared with those before operation(P<0.001).The level of serum P53 antibodies decreased gradually postoperation in the two groups,especially in group B(Fgroup=52.735,Ftime=9.297,Finteraction=4.219,P<0.001).Conclusion:The combination of preoperation infusion chemotherapy and hyperthermia could enhance the intermediate and advanced NSCLC patients’ immunity.

参考文献/References:

[1]李彩霞,曹弛.经肝动脉栓塞化疗对细胞免疫状态的影响[J].中国肿瘤临床,2003,30(9):683

[2]Ohguri T,Imada H,Yahara K,et al.Radiotherapy with 8MHz radiofrequencycapacitive regional hyperthermia for stage Ⅲ nonsmallcell lung cancer:the radiofrequencyoutput power correlates with the intraesophageal temperature and clinical outcomes[J].Int J Radiat Oncol Biol Phys,2009,73(1):128

[3]Ohguri T,Imada H,Narisada H,et al.Systemic chemotherapy using paclitaxel and carboplatin plus regional hyperthermia and hyperbaric oxygen treatment for nonsmall cell lung cancer with multiple pulmonary metastases:preliminary results[J].Int J Hyperthermia,2009,25(2):160

[4]王颖.浅谈临床肿瘤热疗中若干问题[J].中华放射肿瘤学杂志,2004,13(1):25

[5]蒋卫红.高频热疗治疗前列腺疾病的护理体会[J].中国健康月刊A,2011,30(3):211

[6]Ren D,Kim H,Tu HC,et al.The VDAC2BAK rheostat controls thymocyte survival[J].Sci Signal,2009,2(85):ra48

[7]Blazichova S,Rorersky J,Koska J,et al.Effect of hyperthermia water bath on parameters of cellular immunity[J].Int J Clin Pharmocol Res,2000,20(1):41

[8]Agarwal ML,Taylor WR,Chernov MV,et al.The p53 network[J].J Biol Chem,1998,273(1):1

[9]韩毅,许绍发.血清p53抗体与肺癌的研究进展[J].中国肺癌杂志,2003,6(5):406

[10]Shih CM,Chen K,Wang YC,et al.Elevated p53 and p21waf1 mRNA expression in blood lymphocytes from lung cancer patients with chemoresistance[J].Cancer Detect Prev,2007,31(5):366

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备注/Memo

备注/Memo:
△男,1976年9月生,硕士研究生,主治医师,研究方向:胸部肿瘤的外科治疗,Email:zhyxkyy@163.com
更新日期/Last Update: 2013-07-03