[1]刘宗文)△,王文娟),田薇薇),等.食管鳞状细胞癌组织中Netrin1的表达*[J].郑州大学学报(医学版),2013,(01):8.
 LIU Zongwen,WANG Wenjuan,TIAN Weiwei,et al.Expression of Netrin1 in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(01):8.
点击复制

食管鳞状细胞癌组织中Netrin1的表达*
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年01期
页码:
8
栏目:
食管癌研究
出版日期:
2013-01-20

文章信息/Info

Title:
Expression of Netrin1 in esophageal squamous cell carcinoma tissue
作者:
刘宗文1)王文娟2)田薇薇1)梁冰1)杨家梅1)刘萍1)张钰浩3)张艳3)
1)郑州大学第二附属医院肿瘤科 郑州 450014 2)新乡医学院第三附属医院神经内科 新乡 453000 3)郑州大学基础医学院药理学教研室 郑州 450001
Author(s):
LIU Zongwen1)WANG Wenjuan2)TIAN Weiwei1)LIANG Bing1)YANG Jiamei1)LIU Ping1)ZHANG Yuhao3)ZHANG Yan3)
1)Department of Oncology,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014 2)Department of Neurology,the Third Affiliated Hospital,Xinxiang Medical University,Xinxiang 453000 3)Department of Pharmacology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001
关键词:
食管鳞状细胞癌Netrin1
Keywords:
esophageal squamous cell carcinomaNetrin1
分类号:
R735.1
摘要:
目的:探讨Netrin1在食管鳞状细胞癌(ESCC)组织中的表达情况及其与ESCC发生发展的关系。方法:采用免疫组化和原位杂交法分别检测40例ESCC及40例正常食管黏膜组织中Netrin1 蛋白和mRNA的表达。结果:ESCC组织中Netrin1蛋白的阳性表达率为47.5%(19/40),高于正常食管黏膜组织的5.0%(2/40)(χ2=18660,P<0.001)。ESCC组织中Netrin1 mRNA的阳性表达率为52.5%(21/40),高于正常食管黏膜组织的50%(2/40)(χ2=22.029,P<0.001)。Netrin1 mRNA和蛋白的表达与ESCC的组织学分级、浸润深度、淋巴结转移以及TNM分期均有关(χ2mRNA=12.420、12.477、9.528、10.566,χ2蛋白=8.878、8.021、8.338、9.950,P均<0.05)。ESCC组织中Netrin1蛋白与mRNA的表达有关联(rP=0.517,P<0.001)。结论:Netrin1表达与ESCC的发生发展有关,可作为ESCC预后判断的辅助指标。
Abstract:
Aim:To explore the correlation of Netrin1 mRNA and protein expression with the occurrence and development of esophageal squamous cell carcinoma(ESCC).Methods:PV9000 immunohistochemical method and in situ hybridization method were used to detect the expression of Netrin1 protein and mRNA in 40 cases of ESCC and 40 cases of normal esophageal mucosa.Results:Positive expression rate of Netrin1 protein was 47.5%(19/40)in ESCC tissue,which was higher than that(5.0%,2/40)in normal esophageal epithelial tissue(χ2=18.660,P<0.001).Positive expression rate of Netrin1 mRNA was 52.5%(21/40)in ESCC tissue,which was higher than that(5.0%,2/40)in normal esophageal epithelial tissue(χ2=22.029,P<0.001).Expressions of Netrin1 mRNA and protein were associated with histological grade,invasive depth,lymph node metastasis and TNM staging (χ2mRNA=12.420,12.477,9.528,10.566,χ2protein=8.878,8.021,8.338,9.950,P<0.05).There was positive correlation between the protein and mRNA expressions of Netrin1 in ESCC tissue(rP=0.517,P<0.001).Conclusion:Expression of Netrin1 has relation with pathogenesis and development of ESCC and maybe a new assistant marker for prognosis of ESCC.

参考文献/References:

[1]Guenebeaud C,Goldschneider D,Castets M,et al.The dependence receptor UNC5H2/B triggers apoptosis via PP2Amediated dephosphorylation of DAP kinase[J].Mol Cell,2010,40(6):863
[2]Paradisi A,Maisse C,Coissieux MM,et al.Netrin1 upregulation in inflammatory bowel diseases is required for colorectal cancer progression[J].Proc Natl Acad Sci USA,2009,106(40):17146
[3]DelloyeBourgeois C,Brambilla E,Coissieux MM,et al.Interference with netrin1 and tumor cell death in nonsmall cell lung cancer[J].J Natl Cancer Inst,2009,101(4):237
[4]Li SL,Gao DL,Zhao ZH,et al.Correlation of matrix metalloproteinase suppressor genes RECK,VEGF,and CD105 with angiogenesis and biological behavior in esophageal squamous cell carcinoma[J].World J Gastroenterol,2007,13(45):6076
[5]庞霞,李晟磊,赵志华,等.食管鳞状细胞癌组织中FAK、Paxillin和MMP9蛋白的表达及临床意义[J].重庆医学,2011,40(14):1367
[6]Ishii N,Wadsworth WG,Stern BD,et al.UNC6,a lamininrelated protein,guides cell and pioneer axon migrations in C.elegans[J].Neuron,1992,9(5):873
[7]Nakashiba T,Ikeda T,Nishimura S,et al.NetrinG1:a novel glycosyl phosphatidylinositollinked mammalian netrin that is functionally divergent from classical netrins[J].J Neurosci,2000,20(17):6540
[8]Nakashiba T,Nishimura S,Ikeda T,et al.Complementary expression and neurite outgrowth activity of netrinG subfamily members[J].Mech Dev,2002,111(1/2):47
[9]KeinoMasu K,Masu M,Hinck L,et al.Deleted in colorectal cancer(DCC)encodes a netrin receptor[J].Cell,1996,87(2):175
[10]Leonardo ED,Hinck L,Masu M,et al.Vertebrate homologues of C.elegans UNC5 are candidate netrin receptors[J].Nature,1997,386(6627):833
[11]Qin S,Yu L,Gao Y,et al.Characterization of the receptors for axon guidance factor netrin4 and identification of the binding domains[J].Mol Cell Neurosci,2007,34(2):243
[12]Lim YS,Mallapur S,Kao G,et al.Netrin UNC6 and the regulation of branching and extension of motoneuron axons from the ventral nerve cord of Caenorhabditis elegans[J].J Neurosci,1999,19(16):7048
[13]DelloyeBourgeois C,Brambilla E,Coissieux MM,et al.Interference with netrin1 and tumor cell death in nonsmall cell lung cancer[J].J Natl Cancer Inst,2009,101(4):237
[14]Fitamant J,Guenebeaud C,Coissieux MM,et al.Netrin1 expression confers a selective advantage for tumor cell survival in metastatic breast cancer[J].Proc Natl Acad Sci USA,2008,105(12):4850
[15]Papanastasiou AD,Pampalakis G,Katsaros D,et al.Netrin1 overexpression is predictive of ovarian malignancies[J].Oncotarget,2011,2(5):363

相似文献/References:

[1]孟青)),刘书漫),王一菱),等.食管鳞状细胞癌组织中B7H1蛋白和mRNA的表达*[J].郑州大学学报(医学版),2009,(01):48.
 MENG Qing)),LIU Shuman),WANG Yiling),et al.Expression of B7H1 mRNA and protein in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):48.
[2]吴欣爱)△,樊青霞),李鑫),等.乏氧对EC9706细胞多药耐药相关蛋白表达的影响[J].郑州大学学报(医学版),2009,(01):51.
 WU Xinai),FAN Qingxia),LI Xin),et al.Effect of hypoxia on expression of MRP1 and the mechanism in human EC9706 cell line[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):51.
[3]郑湘予,庞霞,李晟磊,等.食管鳞状细胞癌组织中CXCR4蛋白及mRNA的表达*[J].郑州大学学报(医学版),2009,(03):475.
 ZHENG Xiangyu,PANG Xia,LI Shenglei,et al.Detection of CXCR4 protein and mRNA in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):475.
[4]亓民)),李建生)#,马军),等.食管鳞状细胞癌组织中Ca125、Ca153、Ca199和Ca724的表达[J].郑州大学学报(医学版),2009,(03):478.
 QI Min)),LI Jiansheng),MA Jun),et al.Expressions of Ca125, Ca153, Ca199 and Ca724 in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):478.
[5]王琼叶),侯桂琴),王莉莉),等.雷帕霉素抑制mTOR信号通路对EC9706细胞生长及凋亡的影响*[J].郑州大学学报(医学版),2010,(03):356.
 WANG Qiongye),HOU Guiqin),WANG Lili),et al.Effects of rapamycin inhibiting mTOR signaling pathway on growth and apoptosis of esophageal squamous cell carcinoma cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(01):356.
[6]陆玉成,闫红霞,王莉莉,等.自分泌运动因子对EC9706、Eca109及EC1细胞丝切蛋白磷酸化的影响[J].郑州大学学报(医学版),2010,(04):537.
[7]高萍△,臧文巧,王涛.食管鳞状细胞癌组织中Livin与半胱氨酸蛋白酶3的表达[J].郑州大学学报(医学版),2010,(06):888.
[8]孙淼淼,于庆凯,马杰,等.食管鳞状细胞癌组织中Stathmin蛋白及mRNA的表达[J].郑州大学学报(医学版),2010,(06):891.
[9]周胜理),王立东)﹟,岳文彬),等.同卵双胞胎食管鳞状细胞癌患者7对临床病理特征[J].郑州大学学报(医学版),2011,(01):123.
 [J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(01):123.
[10]谷博,吉庆春#,赵富周,等.食管鳞状细胞癌组织中Pin1和mTOR蛋白的表达[J].郑州大学学报(医学版),2012,(02):150.
 GU Bo,JI Qingchun,ZHAO Fuzhou,et al.Expressions of Pin1 and mTOR in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(01):150.

备注/Memo

备注/Memo:
*河南省科技攻关基金资助项目112102310200 △男,1966年12月生,博士,副主任医师,研究方向:肿瘤分子生物学,Email:liuzwhh@sina.com
更新日期/Last Update: 2013-04-19