[1]黄丽艳),王娜),汤黎明),等.PTEN基因表达对EC9706细胞增殖、侵袭、凋亡能力的影响*[J].郑州大学学报(医学版),2013,(01):16.
 HUANG Liyan,WANG Na,TANG Liming,et al.Effects of PTEN on proliferation,invasion and apoptosis in EC9706 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(01):16.
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PTEN基因表达对EC9706细胞增殖、侵袭、凋亡能力的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年01期
页码:
16
栏目:
食管癌研究
出版日期:
2013-01-20

文章信息/Info

Title:
Effects of PTEN on proliferation,invasion and apoptosis in EC9706 cells
作者:
黄丽艳1)王娜2)汤黎明3)许燕3)黄幼田3)杨红艳3)董子明3)#
1)辽宁医学院卫生所 锦州 121001 2)郑州大学基础医学院微生物学与免疫学教研室 郑州 450001 3)郑州大学基础医学院病理生理学教研室 郑州 450001
Author(s):
HUANG Liyan1)WANG Na2)TANG Liming3)XU Yan3)HUANG Youtian3)YANG Hongyan3)DONG Ziming3)
1)Clinic Center,Liaoning Medical College,Jinzhou 121001 2)Department of Microbiology and Immunology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001 3)Department of Pathophysiology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001
关键词:
PTENEC9706细胞增殖细胞侵袭凋亡食管癌
Keywords:
PTENEC9706cell proliferationcell invasionapoptosisesophageal carcinoma
分类号:
R735.1
摘要:
目的:观察PTEN基因表达水平的变化对食管癌EC9706细胞增殖、侵袭、凋亡能力的影响。方法:构建2个靶向PTEN的siRNA载体及1个PTEN表达载体,分别转染EC9706细胞。实验分沉默1组、沉默2组、siRNA载体对照组、PTEN表达组、表达载体对照组和未转染组。RTPCR、Western blot法检测转染后各组细胞PTEN基因mRNA和蛋白表达水平。采用CCK8试验、软琼脂克隆形成实验、流式细胞术和Transwell试验分别检测各组细胞增殖、克隆形成能力、细胞凋亡率及侵袭能力。结果:与未转染组比较,沉默1组、沉默2组PTEN mRNA和蛋白表达量均降低,PTEN表达组PTEN mRNA相对表达量升高(F=25.762,P<0.001);与未转染组比较,PTEN表达组细胞软琼脂克隆形成数和穿透细胞数均降低,细胞凋亡率增加(F=50.752、29.981、32.193,P均<0.001)。结论:上调PTEN的表达水平可以有效抑制EC9706细胞的增殖、克隆形成和侵袭转移能力,同时促进细胞凋亡。
Abstract:
Aim:To observe the effects on the proliferation,invasion and apoptosis ability in EC9706 cells when PTEN expression level being changed.Methods:Two PTEN siRNA vectors and one PTEN expression vector were constructed and transfected into EC9706 cells respectively.The experimental cells were divided into silent group 1,silent group 2,siRNA blank control,PTEN group,PTEN vector control,and untransfected group.mRNA and protein expressions of PTEN were measured by RTPCR and Western blot assay.CCK8 assay,colony formation assay,flow cytometry and Transwell migration assay were employed to assess the ability of proliferation,colonyforming,apoptosis and migration of EC9706 cells when PTEN expression level was changed.Results:Compared with untransfected group,PTEN mRNA and protein expression levels of silent group 1 and silent group 2 were significantly decreased while that of PTEN group was significantly increased (F=25.762,P<0.001).Compared with untransfected group,the ability of cell proliferation,colonyforming and migration significantly decreased and apoptosis rate increased in PTEN group(F=50.752,29.981,32.193,P<0.001).Conclusion:Upregulation of PTEN expression could inhibit EC9706 cells’ growth,proliferation,migration and promote apoptosis.

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备注/Memo

备注/Memo:
*河南省重大公益科研基金资助项目2011 #通讯作者,男,1953年4月生,博士,教授,研究方向:病理生理学,Email:dongzm@zzu.edu.cn
更新日期/Last Update: 2013-04-19