[1]张剑),李建生)#,吴敏),等.胃腺癌组织中SLP2的表达及其对SGC7901细胞增殖能力的影响[J].郑州大学学报(医学版),2013,(01):73.
 ZHANG Jian,LI Jiansheng,WU Min,et al.Expression of SLP2 in gastric adenocarcinoma tissue and its effects on proliferation of SGC7901 cell line[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(01):73.
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胃腺癌组织中SLP2的表达及其对SGC7901细胞增殖能力的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年01期
页码:
73
栏目:
论著
出版日期:
2013-01-20

文章信息/Info

Title:
Expression of SLP2 in gastric adenocarcinoma tissue and its effects on proliferation of SGC7901 cell line
作者:
张剑12)李建生1)#吴敏2)赵晔1)张自森2)
1)郑州大学第一附属医院消化内科 郑州450052 2)郑州大学第五附属医院肿瘤科 郑州 450052
Author(s):
ZHANG Jian12)LI Jiansheng1)WU Min2)ZHAO Ye1)ZHANG Zisen2)
1)Department of Gastroenterology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 2)Department of Oncology,the Fifth Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
胃腺癌SLP2RNA干扰小干扰RNA细胞增殖细胞周期
Keywords:
gastric adenocarcinomaSLP2RNA interferencesiRNAcell proliferationcell cycle
分类号:
R735.2
摘要:
目的:探讨胃腺癌组织中SLP2蛋白的表达情况及沉默SLP2对胃腺癌SGC7901细胞增殖的影响。方法:采用免疫组化法检测45例胃腺癌患者的癌组织、配对癌旁正常胃黏膜组织中SLP2蛋白的表达情况,分析其与临床病理特征的关系;将SLP2 siRNA转染SGC7901细胞,采用MTT法和流式细胞术检测转染后细胞的增殖状况及细胞周期。结果:SLP2蛋白在胃腺癌组织中的阳性表达率(68.9%,31/45)高于癌旁正常组织(26.7%,12/45)(χ2=15.429,P<0.001)。胃腺癌组织中SLP2的表达与淋巴结转移及TNM分期密切相关(校正χ2=4.784,5318,P均<0.05)。SLP2 siRNA转染SGC7901细胞后,细胞增殖活性降低;G1细胞期增多,S期细胞减少(F=14459,11.825,P均<0.05)。结论:SLP2参与了胃腺癌的发生、发展。
Abstract:
Aim:To investigate the expression of SLP2 in gastric adenocarcinoma and its effects on proliferation of SGC7901 cell line.Methods:The expression of SLP2 in 45 cases of gastric adenocarcinoma tissue and adjacent tumorfree tissue was detected by immunohistochemistry,and its association with clinical pathological characteristics was also analyzed.SLP2 siRNA was designed and transfected into gastric adenocarcinoma cell line SGC7901.The cell proliferation was detected by MTT assay,and the cell cycles was analyzed by flow cytometry.Results:The positive rate of SLP2 expression in gastric adenocarcinoma was 68.9%(31/45),which was significantly higher than that of the adjacent tumorfree tissue(267%,12/45)(χ2=15.429,P<0.001).The expression of SLP2 in gastric adenocarcinoma tissue was associated with lymph nodes metastasis and TNM staging(χ2=4784,5.318;both P<0.05).After SLP2 siRNA was transfected,the number of cells in G1 phase was increased and S phase was declined(F=14.459,11.825;both P<0.05).Conclusion:SLP2 protein shows upregulation in gastric adenocarcinoma,and it may have some relationship with carcinogenesis and development of gastric adenocarcinoma.

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备注/Memo

备注/Memo:
#通讯作者,男,1955年10月生,博士,教授,研究方向:消化道肿瘤,Email:lijiansheng@medmail.com.cn
更新日期/Last Update: 2013-04-19