[1]李敏,王建生#,刘东伟.霉酚酸酯对糖尿病大鼠肾组织中IGF1、MMP2表达的影响[J].郑州大学学报(医学版),2013,(01):77.
 LI Min,WANG Jiansheng,LIU Dongwei.Effect of mycophenolate mofetil on insulinlike growth factor1 and matrix metalloproteinase2 in the kidneys of diabetic rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(01):77.
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霉酚酸酯对糖尿病大鼠肾组织中IGF1、MMP2表达的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年01期
页码:
77
栏目:
论著
出版日期:
2013-01-20

文章信息/Info

Title:
Effect of mycophenolate mofetil on insulinlike growth factor1 and matrix metalloproteinase2 in the kidneys of diabetic rats
作者:
李敏王建生#刘东伟
郑州大学第二附属医院肾内科 郑州 450014
Author(s):
LI MinWANG JianshengLIU Dongwei
Department of Nephrology,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014
关键词:
霉酚酸酯胰岛素样生长因子1基质金属蛋白酶2糖尿病肾病
Keywords:
mycophenolate mofetilinsulinlike growth factor1matrix metalloproteinase2diabetic nephropathy
分类号:
R587.1
摘要:
目的:探讨霉酚酸酯(MMF)对糖尿病(DM)大鼠肾组织中胰岛素样生长因子1(IGF1)及基质金属蛋白酶2(MMP2)表达的影响。方法:雄性SD大鼠随机分为对照组(n=12)、糖尿病组(DM组,n=11)、MMF组3组(n=10)。于8周末检测24 h尿蛋白量、血糖、内生肌酐清除率(Ccr)、相对肾质量(左肾质量/体质量);PAS染色观察肾脏病理改变;免疫组化、Western印迹检测肾脏IGF1、MMP2的表达。结果:3组大鼠相对肾质量、血糖、24 h尿蛋白和Ccr差异有统计学意义(F分别为51.097、150.925、161.315和103.307,P<0.001),MMF组相对肾质量、24 h尿蛋白和Ccr均低于DM组,高于对照组(P<0.05)。与对照组比较,DM组肾小球体积增大,系膜区基质明显增生,肾小球毛细血管袢受压,基底膜增厚,而MMF组较DM组有所改善。3组大鼠肾组织IGF1、MMP2蛋白表达差异有统计学意义(F分别为15.715和20.449,P<0.001),DM组中IGF1的表达较对照组增加,MMP2的表达较对照组下降;MMF组IGF1较DM组下降,MMP2增加(P<0.05)。结论:MMF可能通过减少肾脏IGF1的表达,同时上调MMP2表达,起到保护肾脏的作用。
Abstract:
Aim:To investigate the effect of mycophenolate mofetil(MMF) on the expression of insulinlike growth factor1(IGF1) and matrix metalloproteinase2(MMP2) in kidney of diabetic rats.Methods:DM rat models induced by intraperitoneal injection with streptozotocin were constructed.Male SD rats were divided into three groups:normal control rats(control group),diabetic rats(DM group),and diabetic rats received MMF(MMF group).Eight weeks later,24hour proteinuria quantitation,blood glucose,creatine clearance rate(Ccr),and the ratio of kidney weight/body weight were measured,and kidney pathology was observed.Immunohistocytochemistry and Western blot were used to analyze the expression of insulinlike growth factor1(IGF1)and matrix metalloproteinase2(MMP2).Results:The differences on the ratio of kidney weight/body weight,blood glucose,24hour proteinuria quantitation and creatine clearance rate(Ccr) among three groups were statistically significant(F=51.097,150.925,161.315 and 103.307,P<0.001).The ratio of kidney weight/body weight,24hour proteinuria quantitation and creatine clearance rate(Ccr) of MMF group were lower as compared with DM group and increased markedly as compared with the control group.The PAS staining of sectiones of nephridial tissue showed that the degree of glomerular basement membrane thickening,mesangium cell hyperplasia,compression of capillary loop and extracellular matrix hyperplasia of the DM group increased significantly as compared with the Control group.Improvements were observed in the MMF group.The differences on the expression of IGF1 and MMP2 in renal tissue among three groups were statistically significant(F=15.715 and 20.449,P<0.001).Western blot analysis noted that compared with control group the expression of IGF1 in renal tissue of DM group was increased.MMF treatment could reduce the increased expression of IGF1.The expression of MMP2 was on the contrary.Conclusion:The protective role of MMF in diabetic nephropathy which reduce extracellular matrix deposition in renal tissue possibly associated with the decrease of IGF1 and the increase of MMP2.

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备注/Memo

备注/Memo:
#通讯作者,女,1957年11月生,教授,研究方向:肾小球疾病,Email:wjs9857@sina.com
更新日期/Last Update: 2013-04-19