[1]陈杰,张丽华#,牛少辉.缬沙坦对大鼠急性心肌梗死后F肌动蛋白与热休克蛋白27表达的影响[J].郑州大学学报(医学版),2013,(01):84.
 CHEN Jie,ZHANG Lihua,NIU Shaohui.Effects of valsartan on expressions of Factin and HSP27 in acute myocardial infarction rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(01):84.
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缬沙坦对大鼠急性心肌梗死后F肌动蛋白与热休克蛋白27表达的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年01期
页码:
84
栏目:
论著
出版日期:
2013-01-20

文章信息/Info

Title:
Effects of valsartan on expressions of Factin and HSP27 in acute myocardial infarction rats
作者:
陈杰张丽华#牛少辉
郑州大学第二附属医院心血管内科 郑州 450014
Author(s):
CHEN JieZHANG LihuaNIU Shaohui
Department of Vasculocardiology,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014
关键词:
心肌梗死缬沙坦热休克蛋白27F肌动蛋白大鼠
Keywords:
myocardial infarctionvalsartanHSP27Factinrat
分类号:
R541.4
摘要:
目的:通过测定梗死后心肌组织中F肌动蛋白(Factin)、热休克蛋白27(HSP27)蛋白和mRNA的表达,探讨缬沙坦对大鼠急性心肌梗死后细胞骨架蛋白的影响及可能机制。方法:将54只大鼠分为心肌梗死组(MI组,n=11)、缬沙坦组(X组,n=13)、假手术组(SH组,n=15)和正常对照组(N组,n=15)。前2组大鼠结扎冠状动脉左前降支建立急性心肌梗死模型,SH组只穿线不结扎。X组给予缬沙坦(15 mg/kg),1次/d,灌胃治疗4周,其余3组给予等体积生理盐水。4周后HE染色进行心肌组织形态学分析,免疫组化、RTPCR方法测定各组心肌组织中Factin、HSP27蛋白和mRNA的表达。结果:光镜下观察,N组和SH组心肌细胞排列整齐,无肌纤维破坏,MI组和X组可见梗死区心肌细胞坏死,纤维组织增生,X组较MI组梗死面积小,纤维组织增生轻。4组中HSP27、Factin蛋白和mRNA的表达差异有统计学意义(F蛋白=312.760、310.587,P均<0.001;FmRNA=1 527.549、1 290.958,P均<0.001)。与SH组比较,MI组和X组HSP27、Factin蛋白和mRNA表达量升高(P<0.05);MI组HSP27、Factin蛋白和mRNA表达量高于X组(P<0.05)。MI组与X组中HSP27、Factin的表达呈正相关(蛋白:rMI组=0807,P=0003;rX组=0.805,P=0.001;mRNA:rMI组=0.615,P=0.044;rX组=0.762,P=0.002)。结论:缬沙坦可能通过调节细胞骨架蛋白Factin、HSP27的表达而改善心肌梗死后心室重构。
Abstract:
Aim:To investigate the effects of valsartan on the expression of cytoskeletal protein in acute myocardial infarction rats by detecting the expressions of protein and mRNA of Factin and HSP27.Methods:A total of 54 rats were randomly divided into myocardial infarction group(MI group,n=11),valsartan group(X group,n=13),sham operation group(SH group,n=15)and normal control group(N group,n=15).MI group was created by ligation of the left anterior descending coronary artery.X group was given valsartan 15 mg/kg everyday,and the other three groups were given the same amount normal saline by gavage once a day for 4 weeks.Then the hearts of rats were rapidly excised 4 weeks later.And the morphology of the cardiac muscular tissue was observed by HE staining under the optical microscope.The expressions of Factin and HSP27 protein and mRNA were detected by immunohistochemistry and RTPCR,respectively.Results:Pathological observation showed that the myocardial cells in SH and X groups ranged in order,with no fibers fractured.The normal myocytes in MI and X groups were disappeared,and the abruption and disorganization of myocardial fibers in residual myocardium were also observed.It was more serious in MI group than that of X group.There were significant differences in the expressions of HSP27 and Factin protein and mRNA among the four groups(Fprotein=312.760,310.587,P<0.001;FmRNA=1 527.549,1 290.958,P<0.001).The expressions of HSP27 and Factin protein and mRNA and mRNA in MI and X groups were higher than those of SH group and were higher in MI group than those of X group(P<0.05).The expressions of Factin and HSP27 protein and mRNA in MI and X groups had a positive correlation(protein:rMI group=0.807,P=0.003;rX group=0.805,P=0001;mRNA:rMI group=0.615,P=0.044;rX group=0.762,P=0.002).Conclusion:Valsartan may regulate the expressions of Factin and HSP27 and improve the ventricular remodeling after myocardial infarction.

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备注/Memo

备注/Memo:
#通讯作者,女,1964年3月生,硕士,教授,主任医师,研究方向:冠心病的基础与临床,Email:zlhxp@126.com
更新日期/Last Update: 2013-04-19