[1]袁娜娜,卢高峰,唐芙爱#.人结直肠癌组织中肿瘤相关巨噬细胞的检测[J].郑州大学学报(医学版),2013,(01):106.
 YUAN Nana,LU Gaofeng,TANG Fuai.Detection of tumorassociated macrophages in colorectal cancer tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(01):106.
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人结直肠癌组织中肿瘤相关巨噬细胞的检测
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年01期
页码:
106
栏目:
应用研究
出版日期:
2013-01-20

文章信息/Info

Title:
Detection of tumorassociated macrophages in colorectal cancer tissue
作者:
袁娜娜卢高峰唐芙爱#
郑州大学第二附属医院消化内科 郑州 450014
Author(s):
YUAN NanaLU GaofengTANG Fu’ai
Department of Gastroenterology,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014
关键词:
结直肠癌巨噬细胞CD68
Keywords:
colorectal cancermacrophageCD68
分类号:
R735.4
摘要:
目的:肿瘤相关巨噬细胞(TAMs)在人结直肠癌组织中的定量与分布检测。方法:采用Realtime PCR对30例人结直肠癌手术切除标本及15例内镜取材的正常结直肠标本中TAMs标记物CD68 mRNA表达水平进行定量检测,应用免疫组织化学方法检测标本中TAMs的组织分布。分析结直肠癌手术切除标本中CD68 mRNA的表达与临床病理参数之间的关系。结果:CD68 mRNA在结直肠癌组织中的表达高于正常结直肠组织(t=2.720,P<0.001)。不同临床病理参数的结直肠癌组织中CD68 mRNA水平差异无统计学意义(P>0.05)。TAMs分布于结直肠癌组织间质和肿瘤上皮细胞中。结论:TAMs高水平分布于结直肠癌组织间质和肿瘤上皮细胞中。
Abstract:
Aim:To detect the level of tumorassociated macrophages(TAMs)in human colorectal cancer(CRC)tissues.Methods:The expression level of TAMs marker CD68 mRNA was quantified by Realtime PCR in 30 cases of human surgical specimens from CRC and 15 cases of normal colorectal tissue from endoscope.The relationship between the expression of CD68 mRNA and clinicopathological parameters was analyzed in CRC tissue.The distribution of TAMs in CRC tissue was confirmed with the CD68 monoclonal antibody staining.Results:The expression level of CD68 mRNA in CRC tissues was higher than those in normal colorectal tissue(t=2.720,P<0.001).CD68 mRNA level in CRC tissue from different clinlica pathological parameters was not significant(P>0.05).TAMs distributed in the stroma of the tumor tissue and the epithelium cells of CRC tissue.Conclusion:There are more TAMs in stroma tissue and epithelium cells of CRC.

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备注/Memo

备注/Memo:
#通讯作者,女,1952年5月生,本科,教授,研究方向:消化道肿瘤,Email:tangfuai@126.com
更新日期/Last Update: 2013-04-19