[1]景坤玉),董腾慧),郭明洲),等.食管鳞状细胞癌细胞及组织中钙敏感受体基因启动子区甲基化状态检测*[J].郑州大学学报(医学版),2013,(02):155.
 JING Kunyu),DONG Tenghui),GUO Mingzhou),et al.Detection of methylation status of promoter region of CASR in cell and tissue of human esophageal squamous cell carcinoma[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(02):155.
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食管鳞状细胞癌细胞及组织中钙敏感受体基因启动子区甲基化状态检测*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年02期
页码:
155
栏目:
食管癌研究
出版日期:
2013-03-20

文章信息/Info

Title:
Detection of methylation status of promoter region of CASR in cell and tissue of human esophageal squamous cell carcinoma
作者:
景坤玉1)董腾慧2)郭明洲2)薛乐勋1)#
1)郑州大学生物工程系细胞生物学研究室 郑州 4500012)中国人民解放军总医院消化科 北京 100853
Author(s):
JING Kunyu1) DONG Tenghui2) GUO Mingzhou2) XUE Lexun1)
1)Laboratory for Cell Biology, Department of Bioengineering, Zhengzhou University, Zhengzhou 450001 2)Department of Gastroenterology, Chinese PLA General Hospital, Beijing 100853
关键词:
食管鳞状细胞癌表观遗传学DNA甲基化钙敏感受体
Keywords:
esophageal squamous cell carcinomaepigeneticsDNA methylationcalciumsensing receptor
分类号:
R735.1
摘要:
目的:研究钙敏感受体(CASR)基因启动子区甲基化状态与食管鳞状细胞癌(ESCC)发生之间的关系,探讨CASR甲基化作为潜在的ESCC早期诊断标志物的可能性。方法:采用甲基化特异性PCR(MSP)方法分析6株食管癌细胞系、8例正常食管黏膜组织以及68例原发ESCC组织中CASR启动子区的甲基化状态。采用半定量RTPCR方法分析甲基化酶抑制剂5氮杂2脱氧胞嘧啶核苷(5Aza)处理前后食管癌细胞系中CASR mRNA的表达情况。结果:5株食管癌细胞系(KYSE30、KYSE70、KYSE140、TE8、BIC1)CASR启动子区发生甲基化,而KYSE510未发生甲基化。KYSE30、KYSE140细胞系CASR基因表达缺失,5Aza处理96 h后恢复表达;而KYSE510处理前后均有表达。在68例原发ESCC组织中,CASR启动子区甲基化率为71%(48/68),而在8例正常食管黏膜组织中CASR启动子区均未甲基化(0/8)。CASR启动子区甲基化与ESCC患者的年龄构成(χ2=1.114, P=0.291)、性别(χ2=0.342, P=0.558)、肿瘤位置(χ2=1.209, P=0.546)、临床TNM分期(χ2=0.181, P=0.670)及淋巴结转移(χ2=1.169, P=0.280)无关。结论:CASR基因在ESCC组织中的表达受启动子区甲基化的调控;CASR启动子区甲基化在ESCC组织中频繁发生,可能作为食管癌早期诊断的潜在标志物和治疗靶点并在其发生发展中起重要作用。
Abstract:
Aim: To investigate the promoter region methylation status of calciumsensing receptor(CASR) in esophageal carcinogenesis, and to explore the possibility of CASR promoter methylation as a potential biomarker for human esophageal squamous cell carcinoma (ESCC). Methods: The promoter region methylation of CASR in 6 esophageal cancer cell lines, 8 normal esophageal mucosa and 68 primary ESCC tissue samples were investigated using methylation specific PCR. Semiquantitative RTPCR was used to evaluate CASR expression levels before and after treatment with 5aza2’deoxycytidine (5Aza) in cell lines. Results: Methylation of the CASR promoter region was detected in 5 cell lines(KYSE30, KYSE70, KYSE140,TE8 and BIC1), but not in KYSE510. Loss of CASR expression was found in the methylated cell lines. Reexpression of CASR was induced after 5Aza treatment for 96 h. In 68 cases of human primary ESCC tissue samples, the methylation rate of CASR promoter region was 71%(48/68), but no one was methylated in 8 cases of normal esophageal mucosa(0/8). No association was found between promoter region hypermethylation and age(χ2=1.114, P=0.291), gender(χ2=0.342, P=0.558), tumor location(χ2=1.209, P=0.546), tumor stage(χ2=0.181, P=0.670) or lymph node metastasis(χ2=1.169, P=0.280). Conclusion: The methylated CASR frequently exists in primary human ESCC, suggesting it may be used as a potential early detection biomarker or target for this disease.

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备注/Memo

备注/Memo:
*国家重点基础研究发展计划基金资助项目2010CB912802;国家自然科学基金资助项目81071953#通讯作者,男,1944 年2月生,本科,教授,研究方向:肿瘤标志物与基因工程,Email:xuelx@zzu.edu.cn
更新日期/Last Update: 2013-04-19