[1]王军△,尚凤伟,朱登纳,等.银杏内酯B对缺氧缺血性脑损伤新生大鼠脑组织Caspase3及VEGF mRNA表达的影响*[J].郑州大学学报(医学版),2013,(02):175.
 WANG Jun,SHANG Fengwei,ZHU Dengna,et al.Effects of ginkgolide B on expression of Caspase3 and VEGF mRNA in brain tissue of newborn rats with hypoxicischemic brain damage[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(02):175.
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银杏内酯B对缺氧缺血性脑损伤新生大鼠脑组织Caspase3及VEGF mRNA表达的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年02期
页码:
175
栏目:
论著
出版日期:
2013-03-20

文章信息/Info

Title:
Effects of ginkgolide B on expression of Caspase3 and VEGF mRNA in brain tissue of newborn rats with hypoxicischemic brain damage
作者:
王军尚凤伟朱登纳王宝珍张冬秀张贞焕马洁琼
郑州大学第三附属医院脑瘫康复科 郑州 450052
Author(s):
WANG JunSHANG FengweiZHU DengnaWANG BaozhenZHANG DongxiuZHANG ZhenhuanMA Jieqiong
Department of Rehabilitaion for Cerebral Palsy,the Third Affiliated Hospital, Zhengzhou University,Zhengzhou 450052
关键词:
缺氧缺血性脑损伤新生大鼠 银杏内酯B Caspase3 血管内皮生长因子
Keywords:
hypoxicischemic brain damage newborn rat ginkgolide B Caspase3 vascular endothelial growth factor
分类号:
R722.1
摘要:
目的:观察银杏内酯B (GB)对缺氧缺血性脑损伤(HIBD)新生大鼠脑组织Caspase3及血管内皮生长因子(VEGF)mRNA表达的影响。方法:清洁级新生7 d龄SD大鼠96只,随机分为假手术组、模型组、GB低剂量组及GB高剂量组。后3组采用经典 Rice法制作HIBD动物模型,假手术组不行缺氧处理。模型制备4 h后GB高、低剂量组分别按10、5 mg/kg腹腔注射GB,余2组注射等量生理盐水,1次/d,共5 d。造模后第3、7、14和28天每组分别随机选取6只动物处死,荧光定量PCR法检测脑组织Caspase3及VEGF mRNA的表达。结果:造模后,假手术组各时间点Caspase3和VEGF mRNA表达水平差异均无统计学意义(F=0.134,0.382,P均>0.05),而模型组、GB低和高剂量组Caspase3 和VEGF mRNA的表达均于造模后第3天达最高,此后逐渐下降,至造模后第28天接近假手术组水平(F=109.356,33.281,9.703和39.945,103.903,60.956,P<0.001)。造模后第3、7和14天,4组Caspase3和VEGF mRNA表达水平差异均有统计学意义(F=128.865和97.912,40.058和57.121,24.184和8.621,P均<0.001);与假手术组比较,模型组Caspase3 和VEGF mRNA的表达均升高(P<0.05);与模型组比较,GB低和高剂量组Caspase3 的表达降低,VEGF mRNA的表达升高(P<0.05); GB高剂量组2指标的变化较低剂量组更明显(P<0.05)。结论:GB可下调HIBD后脑组织Caspase3表达及上调VEGF表达,从而减轻并修复脑损伤。
Abstract:
Aim: To observe the effect of ginkgolide B (GB) on mRNA expression of Caspase3 and vascular endothelial growth factor (VEGF) in brain tissue of hypoxicischemic brain damage (HIBD) newborn rats. Methods: A total of 96 clean 7dayold health SD rats were randomly divided into sham operation group, the model group, low and high dose GB treatment groups. Classic Rice method was used to establish HIBD model in the latter 3 groups. 4 h after operation, GB at dose of 5 mg/kg and 10 mg/kg was given to rats in the low and high dose GB treatment groups by intraperitoneal injection postoperation, once a day for 5 days, sham operation and model groups were given equal physiological saline. 6 rats in each group were sacrificed at the 3rd,7th,14th and 28th day after model establishment,respectively. Quantitative realtime fluorescent PCR was employed to detect the expressions of Caspase3 and VEGF mRNA in brain tissue. Results: The expressions of Caspase3 and VEGF mRNA had no significant differences among 4 time points in sham operation group(F=0.134,0.382,P>0.05),but in the model group, low and high dose GB treatment groups, the expressions of Caspase3 and VEGF mRNA reached the peak at the 3rd day,then decreased,and reached the level of the sham operation group at the 28th day after operation(F=109.356,33.281,9.703 and 39.945,103.903,60.956,P<0.001).At the 3rd,7th,14th day after operation, the expressions of Caspase3 and VEGF mRNA had significant differences among the 4 groups(F=128.865 and 97.912,40.058 and 57.121,24.184 and 8.621,P<0.001);compared with sham operation group, the expressions of Caspase3 and VEGF mRNA in model group were higher,while the expression of Caspase3 mRNA was lower and that of VEGF mRNA was higher in low and high dose GB treatment groups when compared with the model group(P<0.05), and the changes of high dose GB treatment group was more significant(P<0.05).Conclusion: GB can repair brain damage after HIBD through downregulating Caspase3 expression and upregulating VEGF expression.

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备注/Memo

备注/Memo:
*河南省教育厅自然科学研究项目2009A320039△女,1963年7月生,博士,教授,主任医师,研究方向:小儿神经疾病的康复治疗,Email:swangjun@zzu.edu.cn
更新日期/Last Update: 2013-04-19