[1]李胜云△,白冰,徐振亚.腹腔灌注冲洗疗法对游离胃癌细胞腹腔转移的预防作用[J].郑州大学学报(医学版),2013,(02):266.
 LI Shengyun,BAI Bing,XU Zhenya.Effect of intraoperative peritoneal lavage on gastric cancer peritoneal metastasis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(02):266.
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腹腔灌注冲洗疗法对游离胃癌细胞腹腔转移的预防作用
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年02期
页码:
266
栏目:
应用研究
出版日期:
2013-03-20

文章信息/Info

Title:
Effect of intraoperative peritoneal lavage on gastric cancer peritoneal metastasis
作者:
李胜云白冰徐振亚
郑州大学第一附属医院医院感染管理科 郑州 450052
Author(s):
LI ShengyunBAI BingXU Zhenya
Department of Hospital Infection Management, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450052
关键词:
胃癌腹腔灌洗癌胚抗原
Keywords:
gastric cancer peritoneal lavage carcinoembryonic antigen
分类号:
R735.2
摘要:
研究术中腹腔灌注冲洗疗法对胃癌细胞腹腔转移的预防作用。方法:对行胃癌切除术且病理证实为腺癌的72例患者按临床病理分期分为7组,在胃癌切除及完成消化道重建后,用37 ℃生理盐水500 mL灌洗腹腔,每位患者共灌洗6次,收集灌洗液。以CD45和CEA为检测指标,采用流式细胞分析技术检测腹腔灌洗液中CD45阴性且CEA 阳性的细胞表达情况,统计灌洗液中的胃癌细胞数量及灌洗液中胃癌细胞数量为0时的灌洗次数。结果:灌洗次数和胃癌分期对胃癌细胞数量的影响存在交互效应(F=25.290,P<0.001),胃癌分期效应差异有统计学意义(F=55.751,P<0.001),胃癌细胞数量随分期进展而增多;胃癌细胞随灌洗次数增多依次减少(P<0.05);ⅡA期、ⅡB期、ⅢA期患者灌洗液中检测胃癌细胞为0的最高灌洗次数为3、6、6次。结论:腹腔灌洗可有效减少腹腔内游离癌细胞,对预防游离癌细胞腹腔转移有重要意义。
Abstract:
Aim:To investigate the preventive effectiveness of intraoperative peritoneal lavage on gastric cancer peritoneal metastasis. Methods:A total of 72 patients undergoing gastrectomy were divided into 7 groups according to clinical stages. Lavaging the peritoneal cavity 6 times with 37 ℃ normal saline. The gastric cancer cells with negative CD45 expression and positive CEA expression were detected by flow cytometry.Results:The times of lavage and tumor stages had an interactive impact on the quantity of gastric cells(F=25.290,P<0.001),the effect of the lavage among different stages had significant difference(F=55.751,P<0.001). Gastric cancer cells increased with tumor stages and decreased with the increase of peritoneal lavage times(P<0.05);patients at stage ⅡA,ⅡB,ⅢA need 3,6,6 times peritoneal lavage respectively to clear up the gastric cancer cells in peritoneal cavity. Conclusions:Peritoneal lavage can effectively reduce peritoneal gastric cancer cells, and has important effect on the prevention of peritoneal metastasis.

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备注/Memo

备注/Memo:
△女,1967年9月生,硕士,副教授,研究方向:腹腔肿瘤术中腹腔灌注冲洗疗法的应用,Email:lishengyun1@163.com
更新日期/Last Update: 2013-04-19