[1]李志勤),孙长宇),余祖江),等.HCV核心蛋白对人肝癌细胞系SMMC7721增殖的影响*[J].郑州大学学报(医学版),2013,(03):310.
 LI Zhiqin),SUN Changyu),YU Zujiang),et al.Effect of HCV core protein on proliferation of human hepatocellular carcinoma cell line SMMC7721[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(03):310.
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HCV核心蛋白对人肝癌细胞系SMMC7721增殖的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年03期
页码:
310
栏目:
论著
出版日期:
2013-06-20

文章信息/Info

Title:
Effect of HCV core protein on proliferation of human hepatocellular carcinoma cell line SMMC7721
作者:
李志勤12)孙长宇1)余祖江1)黄建敏2)李建生3)#张毅2)#
1) 郑州大学第一附属医院感染科 郑州450052
2)郑州大学第一附属医院生物细胞治疗中心 郑州 450052
3)郑州大学第一附属医院消化内科 郑州 450052
Author(s):
LI Zhiqin12)SUN Changyu1) YU Zujiang1) HUANG Jianmin2)LI Jiansheng3) ZHANG Yi2)
1)Department of Infectious Disease,the First Affiliated Hospital, Zhengzhou University,Zhengzhou 450052
2)Biotherapy Center, the First Affiliated Hospital, Zhengzhou University,Zhengzhou 450052
3)Department of Digestive Disease, the First Affiliated Hospital, Zhengzhou University,Zhengzhou 450052
关键词:
肝癌SMMC7721HCV核心蛋白
Keywords:
hepatocellular carcinomaSMMC7721HCV core protein
分类号:
R735.7
摘要:
摘要目的:观察HCV核心蛋白对低侵袭性人肝癌细胞系SMMC7721增殖的影响。方法:将HCV核心蛋白的原核表达质粒pcDNA3.0CEGFP转染SMMC7721细胞,以空质粒pcDNA3.0(-)转染的细胞作对照。分别采用RTPCR、Western blot和ELISA法检测转染细胞HCV核心蛋白mRNA、蛋白及培养上清中蛋白的表达水平;进行纤维连接蛋白黏附实验、侵袭实验及运动实验,MTT法观察细胞增殖情况。 结果:重组质粒pcDNA3.0CEGFP转染的SMMC7721细胞HCV核心蛋白mRNA、蛋白及培养上清中蛋白的表达水平,细胞黏附率,侵袭实验及运动实验穿膜细胞数均高于对照(t=8.141、5.762、19.931、2.961、4.112和4.213,P均<0.05)。pcDNA3.0CEGFP转染的SMMC7721细胞增殖率也较对照明显增加(P<0.05)。结论:HCV核心蛋白外源基因可促进SMMC7721细胞系的增殖。
Abstract:
AbstractAim: To investigate the effect of HCV core protein on the proliferation of human hepatocellular carcinoma cell line SMMC7721.Methods:SMMC7721 cells were transfected with plasmid pcDNA3.0CEGFP,and cells transfected with pcDNA3.0 (-) served as controls. The mRNA expression in the cells, and protein expressions in the cells and in the supernatant of HCV core protein were verified by RTPCR,Western blot and ELISA methods,respectively.A series of functional assays were used to monitor the changes of the cells adhesive, migrant and invasive capabilities,and MTT method was used to detect cell proliferation.Results: The mRNA expression in the cells, and protein expressions in the cells and in the supernatant of HCV core protein, adhesive, migrant and invasive capabilities of the cells transfected with pcDNA3.0CEGFP were higher than those of the control(t=8.141,5.762, 19.931,2.961,4.213,and 4.112,P<0.05).The proliferation rate of the cells transfected with pcDNA3.0CEGFP was higher than that of the control(P<0.05). Conclusion:HCV core protein might enhance the proliferation of SMMC7721 cells.

参考文献/References:

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备注/Memo

备注/Memo:
*国家自然科学基金面上项目81171986;河南省科技创新杰出人才计划基金资助项目124200510006;河南省卫生厅科研基金资助项目201203015
#通讯作者,李建生,男,1955年10月生,主任医师,教授,研究方向:消化道肿瘤,Email:lijiansheng@medmail.com.cn;张毅,男,1964年4月生,教授,研究方向:肿瘤免疫学、肿瘤干细胞和生物细胞治疗,Email:chyizhang@gmail.com
更新日期/Last Update: 2013-07-03