[1]王冲),余建),黄河)#.Plk1磷酸化修饰PinX1对宫颈癌HeLa细胞有丝分裂及凋亡的影响*[J].郑州大学学报(医学版),2013,(03):323.
 WANG Chong),YU Jian),HUANG He).Effects of PinX1 phosphorylation by Plk1 on mitosis and apoptosis of cervical cancer HeLa cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(03):323.
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Plk1磷酸化修饰PinX1对宫颈癌HeLa细胞有丝分裂及凋亡的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年03期
页码:
323
栏目:
论著
出版日期:
2013-06-20

文章信息/Info

Title:
Effects of PinX1 phosphorylation by Plk1 on mitosis and apoptosis of cervical cancer HeLa cells
作者:
王冲1)余建2)黄河2)#
1) 郑州大学第一附属医院血液科 郑州 450052
2) 浙江大学附属第一医院骨髓移植中心 杭州 310003
Author(s):
WANG Chong1)YU Jian2)HUANG He2)
1)Department of Hematology, the First Affiliated Hospital,Zhengzhou University, Zhengzhou 450052
2)Department of Bone Marrow Transplantation,the First Affiliated Hospital, Zhejiang University, Hangzhou 310003
关键词:
Plk1PinX1凋亡磷酸化有丝分裂
Keywords:
Plk1PinX1apoptosisphosphorylationmitosis
分类号:
R730.231
摘要:
摘要目的:探讨有丝分裂激酶Plk1通过磷酸化修饰PinX1调控宫颈癌HeLa细胞有丝分裂及凋亡的作用。 方法:运用酵母双杂交、免疫共沉淀、谷胱甘肽S转移酶沉降实验了解Plk1与PinX1在体内外是否能够相互结合采用;采用体内外磷酸化实验明确Plk1是否能够磷酸化修饰PinX1;采用免疫荧光染色及流式细胞术检测PinX1磷酸化对HeLa细胞有丝分裂及凋亡的影响。 结果:Plk1与PinX1在体内外均能结合;Plk1在体内外均可磷酸化修饰PinX1;过量表达PinX1的非磷酸化突变体能够阻碍HeLa细胞的有丝分裂进程;过量表达PinX1的磷酸化突变体导致细胞凋亡的增加(F=76 554.133,P<0.001)。 结论:Plk1与PinX1相互结合并通过磷酸化修饰PinX1阻碍细胞有丝分裂进程及促进细胞凋亡。
Abstract:
Abstract Aim: To investigate the effects of phosphorylation of PinX1 by Plk1 on mitosis and apoptosis of cervical cancer HeLa cells. Methods:Using yeast hybridation assay, coimmunoprecipitation and GST pulldown assay to determine the domain of Plk1 and PinX1 binding and interaction in vitro and in vivo. To check the phosphorylation of PinX1 by Plk1 using in vitro phosphorylation assay. Immunofluorescence assay and flow cytometry were used to analyze the effect of phosphorylation of PinX1 on apoptosis and mitosis. Results: Plk1 interacted with PinX1 and could be phosphorylated by PinX1 in vivo and in vitro. Overexpression of phosphomimicking mutant of PinX1 in Hela cells resulted in significant mitosis delay and apoptosis(χ2=76 554.133,P<0.001). Conclusion: Plk1 and PinX1 could interacted and the Plk1mediated phosphorylation of PinX1 is essential for mitosis and apoptosis in HeLa cells.

参考文献/References:

[1]Zhou XZ, Lu KP. The Pin2/TRF1interacting protein Pinx1 is a potent telomerase inhibitor[J]. Cell, 2001,107(3):347
[2]Yuan K, Li N, Jiang K, et al. PinX1 is a novel microtubulebinding protein essential for accurate chromosome segregation[J]. J Biol Chem, 2009,284(34):23072
[3]Blasco MA. The epigenetic regulation of mammalian telomeres[J]. Nat Rev Genet, 2007,8(4):299
[4]Smogorzewska A, de Lange T. Regulation of telomerase by telomeric proteins[J]. Annu Rev Biochem, 2004,73:177
[5]de Lange T. Shelterin: the protein complex that shapes and safeguards human telomeres[J]. Genes Dev, 2005,19(18):2100
[6]Yoo JE, Oh BK, Park YN. Human Pinx1 mediates TRF1 accumulation in nucleolus and enhances TRF1 binding to telomeres[J]. J Mol Biol, 2009,388(5):928
[7]Cai MY, Zhang B, He WP, et al. Decreased expression of Pinx1 protein is correlated with tumor development and is a new independent poor prognostic factor in ovarian carcinoma[J]. Cancer Sci,2010,101(6):1543
[8]Zhang B, Bai YX, Ma HH, et al. Silencing Pinx1 compromises telomere length maintenance as well as tumorigenicity in telomerasepositive human cancer cells[J]. Cancer Res, 2009,69(1):75
[9]Wong JM, Kusdra L, Collins K. Subnuclear shuttling of human telomerase induced by transformation and DNA damage[J]. Nat Cell Biol, 2002,4(9):731
[10]Li N, Yuan K, Yan F, et al. Pinx1 is recruited to the mitotic chromosome periphery by Nucleolin and facilitates chromosome congression[J]. Biochem Biophys Res Commun, 2009,384(1):76

相似文献/References:

[1]王冲),王伟琼),余建),等.Plk1磷酸化修饰Tara蛋白对HeLa细胞胞质分裂及增殖的影响*[J].郑州大学学报(医学版),2013,(06):743.
 WANG Chong),WANG Weiqiong),YU Jian),et al.Phosphorylation of Tara by Plk1 regulates proliferation and cytokinesis of HeLa cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(03):743.

备注/Memo

备注/Memo:
*国家自然科学基金资助项目30900640;中国博士后科学基金资助项目2012M511591
#通讯作者,男,1961年6月生,教授,主任医师,研究方向:血液系统恶性肿瘤的临床及基础,Email:hehuang.zju@gmail.com
更新日期/Last Update: 2013-07-03