[1]李莹,张楠,刘芳丽,等.白藜芦醇对铅暴露小鼠学习记忆能力及氧化应激相关因子的影响*[J].郑州大学学报(医学版),2013,(03):327.
 LI Ying,ZHANG Nan,LIU Fangli,et al.Effects of resveratrol on learning memory performance and oxidative stress in lead exposure mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(03):327.
点击复制

白藜芦醇对铅暴露小鼠学习记忆能力及氧化应激相关因子的影响*
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年03期
页码:
327
栏目:
论著
出版日期:
2013-06-20

文章信息/Info

Title:
Effects of resveratrol on learning memory performance and oxidative stress in lead exposure mice
作者:
李莹张楠刘芳丽王雯陈灿映李文杰#
郑州大学公共卫生学院营养与食品卫生学教研室 郑州 450001
Author(s):
LI Ying ZHANG Nan LIU Fangli WANG Wen CHEN Canying LI Wenjie
Department of Nutrition and Food Hygiene,College of Public Health,Zhengzhou University,Zhengzhou 450001
关键词:
白藜芦醇铅暴露氧化应激小鼠
Keywords:
resveratrollead exposureoxidative stressmouse
分类号:
R995
摘要:
摘要目的:探讨白藜芦醇对铅暴露小鼠学习记忆能力及氧化应激相关因子的影响。方法:将50只昆明小鼠随机分成5组,每组10只,即空白对照组、铅暴露组、白藜芦醇低、中、高剂量干预组。自由饮水形式染毒8周后,以Morris水迷宫实验测试小鼠学习记忆能力;用Z5000型石墨炉原子吸收光谱仪测定血铅、海马组织铅含量;测定血清和海马组织中SOD活力和MDA含量。结果:Morris水迷宫实验结果显示,各组小鼠逃避潜伏期比较,差异有统计学意义(F=9.044,P<0.001)。各组小鼠血铅和海马组织铅含量比较,差异均有统计学意义(F=24.142和17.511,P均<0.001);与铅暴露组相比,白藜芦醇干预组海马组织铅含量降低,差异有统计学意义(P<0.05)。各组小鼠血清和海马组织SOD活力比较,差异均有统计学意义(F=35.190和11.933,P均<0.001);各组小鼠血清和海马组织MDA含量比较,差异有统计学意义(F=5.610和4.406,P均<0.05)。与空白对照组比较,铅暴露组和3个白藜芦醇干预组小鼠血清、海马组织SOD活力降低(P<0.05);与铅暴露组相比,白藜芦醇中剂量和高剂量干预组小鼠血清、海马组织SOD活力升高(P<0.05)。结论:白藜芦醇干预可降低铅暴露小鼠血铅、海马组织铅含量,拮抗其对学习记忆能力的损伤作用,提高SOD活力、降低MDA含量。
Abstract:
AbstractAim:To explore the effects of resveratrol (RES) on the learning memory performance and oxidative stress in lead exposure mice. Methods: Fifty Kunming mice were randomly divided into five groups, which were control group, lead exposure only group (Pb), low (L), middle (M), and high (H) dose RES interfering groups. The mice were allowed freely to drink water. After eight weeks feeding, Morris water maze (MWM) test was employed to evaluate the learning memory ability of the mice. The lead levels in blood and hippocampus were analyzed by graphite furnace atomic absorption spectrometry. Serum and hippocampal SOD activity and MDA level were measured. Results: MWM test showed that the escape latency among the 5 groups had significant differences (F=9.044,P<0.001). The lead levels in blood and hippocampus had significant differences (F=24.142 and 17.511,P<0.001).Compared with Pb group,the lead levels in hippocampus of RES interfering groups reduced (P<0.05). The SOD activity in blood and hippocampus showed significant differences among groups (F=35.190 and 11.933,P<0.001). The MDA levels also showed significant differences among groups (F=5.610 and 4.406,P<0.05). Compared with control group,the serum and hippocampal SOD activity significantly decreased in Pb group and the three RES interfering groups (P<0.05). Compared with Pb group,the SOD activity in M and H groups significantly increased (P<0.05). Conclusion: RES could reduce the lead levels in blood and hippocampus, reverse the learning memory damage in lead exposure mice,as well as increase SOD activity and reduce the level of MDA.

参考文献/References:

[1]Plusquellec P, Muckle G, Dewailly E, et al. The relation of lowlevel prenatal lead exposure to behavioral indicators of attention in Inuit infants in Arctic Quebec[J]. Neurotoxicol Teratol, 2007, 29(5):527
[2]Othaman AI, El Missiry MA. Role of selenium against lead toxicity in male rats[J]. J Biochem Mol Toxicol, 1998, 12(6):345
[3]Kim HJ, Chang EJ, Cho SH, et al. Antioxidative activity of resveratrol and its derivatives isolated from seeds of Paeonia lactiflora[J]. Biosci Biotechnol Biochem, 2002, 66(9):1990
[4]李国洪,金美娟,王新鸣,等. 白藜芦醇对氧化型LDL所致血管内皮细胞损伤的保护作用[J]. 解放军医学杂志,2008,33(6):719
[5]任俊伟, 杨琴. 白藜芦醇对脑缺血/再灌注氧化应激损伤保护机制的研究进展[J]. 中国药理学通报, 2011, 27(4):448
[6]高大宽. 白藜芦醇对小鼠脑缺血再灌注损伤的保护作用机制研究[D].西安:第四军医大学, 2006.
[7]陈灿映,冯静,刘芳丽,等.孕哺期铅暴露对仔鼠学习记忆能力及海马组织中PJNK表达的影响[J].郑州大学学报:医学版,2012,47(1):78
[8]刘欣欣,李宁,郑艳涛,等. 母鼠孕哺期铅暴露对仔鼠学习记忆能力及神经反射的影响[J]. 实用儿科临床杂志,2009,24(19):1519
[9]沈晓明, 颜崇淮, 吴圣楣, 等. 胎儿期低水平铅暴露对婴儿发育影响的前瞻性研究[J]. 中华儿科杂志, 1997, 35(4):174
[10]林茂. 白黎芦醇对阿尔茨海默病小鼠Aβ表达的影响[D].贵州:遵义医学院, 2009.
[11]李燕玲, 王文敏. 白藜芦醇抗氧化作用在神经保护中的研究进展[J]. 国际神经病学神经外科杂志, 2010, 37(2):154
[12]Jurczuk M, MoniuszkoJakoniuk J, Brzoska MM. Involvement of some lowmolecular thiols in the peroxidative mechanisms of lead and ethanol action on rat liver and kidney[J]. Toxicology, 2006, 219(1/3):11
[13]徐冰,林焕冰,周恒,等.白藜芦醇苷对PC12细胞氧糖剥夺损伤模型的保护作用[J].南方医科大学学报,2010,30(5):1041

相似文献/References:

[1]李晓芳,柴雅玫,文小岗,等.白藜芦醇对BGC823细胞人程序化死亡分子5表达及凋亡的影响[J].郑州大学学报(医学版),2010,(06):943.
[2]单中杰),郭亮),侯箐岚),等.白藜芦醇对人肾细胞癌7860细胞周期、凋亡及PDCD5 mRNA表达的影响*[J].郑州大学学报(医学版),2012,(02):182.
 SHAN Zhongjie),GUO Liang),HOU Qinglan),et al.Effects of reservatrol on cell cycle, apoptosis and expression of PDCD5 mRNA in 7860 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(03):182.
[3]马曜辉),单中杰)#.白藜芦醇对膀胱移行细胞癌T24细胞凋亡及组织蛋白酶B表达的影响*[J].郑州大学学报(医学版),2014,(04):498.
 MA Yaohui),SHAN Zhongjie).Effect of resveratrol on cell apoptosis and expression of cathepsin B in T24 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(03):498.
[4]王欣欣),马珊珊),孟 楠),等.hUC-MSCs和白藜芦醇对AD小鼠学习记忆能力及脑内SIRT1信号通路的影响*[J].郑州大学学报(医学版),2016,(05):576.
 WANG Xinxin),MA Shanshan),MENG Nan),et al.Effects of hUC-MSCs and resveratrol on learning and memory capability and SIRT signaling pathway in brain tissue of AD mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(03):576.

备注/Memo

备注/Memo:
*国家自然科学基金资助项目81172716
#通讯作者,男,1958年11月生,博士,教授,研究方向:铅中毒机制,Email:lwj@zzu.edu.cn
更新日期/Last Update: 2013-07-03