[1]贾要丽),吴拥军),吴秋歌),等.肺癌患者RASSF1A和FHIT基因启动子区甲基化检测*[J].郑州大学学报(医学版),2013,(03):373.
 JIA Yaoli),WU Yongjun),WU Qiuge),et al.Detection of promoter methylation of RASSF1A and FHIT genes in patients with lung cancer[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(03):373.
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肺癌患者RASSF1A和FHIT基因启动子区甲基化检测*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年03期
页码:
373
栏目:
应用研究
出版日期:
2013-06-20

文章信息/Info

Title:
Detection of promoter methylation of RASSF1A and FHIT genes in patients with lung cancer
作者:
贾要丽1)吴拥军2)吴秋歌1)张佳1)马东波1)吴逸明2)王静1)#
1)郑州大学第一附属医院呼吸与危重症医学科 郑州 450052
2)郑州大学公共卫生学院毒理学教研室 郑州 450001
Author(s):
JIA Yaoli1)WU Yongjun2)WU Qiuge1)ZHANG Jia1)MA Dongbo1)WU Yiming2)WANG Jing1)
1)Department of Respiratory Disease and Critical Care Medicine,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
2)Department of Toxicology,College of Public Health,Zhengzhou University,Zhengzhou 450001
关键词:
RASSF1AFHIT甲基化肺癌
Keywords:
RASSF1AFHITmethylationlung cancer
分类号:
R734.2
摘要:
摘要目的:探讨外周血RASSF1A 和FHIT基因启动子区甲基化与肺癌发生发展的关系。方法:应用实时荧光定量甲基化特异PCR(qMSP)方法检测200例肺癌患者和200例健康对照人群外周血RASSF1A和FHIT基因启动子区的甲基化率,分析其在2组人群中甲基化水平的差异,并分析年龄、性别、吸烟史、组织学类型及临床分期对基因启动子区甲基化率的影响。结果:2组外周血RASSF1A和FHIT基因启动子区甲基化率的差异有统计学意义(RASSF1A: Z=2.075,P=0.038;FHIT: Z=3.044,P=0.002)。肺癌患者的性别、年龄和吸烟史、组织学类型及临床分期与RASSF1A和FHIT基因启动子区甲基化无关。随着RASSF1A和FHIT基因启动子区甲基化水平增加,患肺癌的危险性增加[RASSF1A:β=0.235,OR(95%CI)=1.551(1.023~2.353);FHIT:β=0.091,OR(95%CI)=1.763(1.116~2.671)]。结论:RASSF1A和FHIT基因启动子区的甲基化与肺癌有关,检测外周血中RASSF1A和FHIT基因启动子区甲基化水平可能有助于肺癌的早期预警。
Abstract:
AbstractAim: To investigate the relationship between promoter methylation status of cancer suppressor genes fragile histidine triad (FHIT)and ras association domain family 1A (RASSF1A) in peripheral blood and the development of lung cancer. Methods:The blood samples of 200 lung cancer patients and 200 normal controls were collected to determine the methylation levels of RASSF1A and FHIT using realtime PCR. Results: For both genes, there were significant differences in the methylation levels between lung cancer patients and controls (RASSF1A: Z=2.075,P=0.038;FHIT: Z=3.044,P=0.002). There were no associations between gender,age, smoking history, histological type,or clinical stage with RASSF1A and FHIT gene promoter methylation in the lung cancer group.The risk of lung cancer increased with the in creased of methylation levels of RASSF1A and FHIT genes[RASSF1A:β=0.235,OR(95%CI)=1.551(1.023-2.353);FHIT:β=0.091,OR(95%CI)=1.763(1.116-2.671)]. Conclusion: The aberrant promoter methylation of RASSF1A and FHIT is related to lung cancer.To detect RASSF1A and FHIT gene aberrant promoter methylation levels may contribute to early warning of lung cancer and the prediction of lung cancer risk.

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目30972457
#通讯作者,女,1958年6月生,博士,教授,研究方向:呼吸系统基础疾病及肺癌的早期预警,Email:wangjing@zzu.edu.cn
更新日期/Last Update: 2013-07-03