[1]田芳)△,江亚南),张晓艳),等.辣椒素对食管鳞状细胞癌细胞凋亡和NFκB信号通路相关蛋白表达的影响*[J].郑州大学学报(医学版),2013,(04):437.
 TIAN Fang,JIANG Yanan,ZHANG Xiaoyan,et al.Effects of capsaicin on expressions of NFκB signaling pathwayrelated proteins and apoptosis of esophageal squamous cell carcinoma cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(04):437.
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辣椒素对食管鳞状细胞癌细胞凋亡和NFκB信号通路相关蛋白表达的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年04期
页码:
437
栏目:
食管癌研究
出版日期:
2013-07-20

文章信息/Info

Title:
Effects of capsaicin on expressions of NFκB signaling pathwayrelated proteins and apoptosis of esophageal squamous cell carcinoma cells
作者:
田芳1江亚南1张晓艳1范天黎2
1)郑州大学基础医学院病理生理学教研室 郑州 4500012)郑州大学基础医学院药理学教研室 郑州 450001
Author(s):
TIAN Fang1)JIANG Yanan1)ZHANG Xiaoyan1)FAN Tianli2)
1)Department of Pathophysiology,College of Basic Medical Sciences,Zhengzhou University, Zhengzhou 4500012)Department of Pharmacology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001
关键词:
食管鳞状细胞癌辣椒素NFκBIκBαBcl2EC9706Eca109
Keywords:
esophageal squamous cell carcinomacapsaicinNFkappa BIκBαBcl2EC9706Eca109Abstract
分类号:
R735.1
摘要:
目的:探讨辣椒素对食管鳞状细胞癌(食管鳞癌)细胞凋亡和NFκB信号通路的影响。方法:采用Western blot法检测食管鳞癌EC9706和Eca109细胞中IκBα、磷酸化IκBα(pIκBα)、p50和p65蛋白的表达及0、50、100和200 μmol/L辣椒素作用后pIκBα和Bcl2蛋白的表达;凝胶滞留实验检测2种食管鳞癌细胞胞核中NFκB与DNA的结合活性及0、100和200 μmol/L辣椒素作用对NFκB与DNA结合活性的影响。2种食管鳞癌细胞分别分为对照组、5FU组、辣椒素组和联合组,流式细胞仪检测各组细胞凋亡情况。结果:2种食管鳞癌细胞胞质均表达IκBα和pIκBα蛋白,胞核均表达p50和p65蛋白;随辣椒素作用浓度的增加,pIκBα和Bcl2蛋白的表达逐渐下降,NFκB与DNA的结合活性也逐渐下降。辣椒素可促进2种食管鳞癌细胞的凋亡,与5FU 联合使用时,凋亡细胞显著增加(EC9706:F辣椒素=2 535.497,F交互=113.034,P<0.001;Eca109:F辣椒素=5 984.040,F交互=5.150,P<0.05)。结论:辣椒素可能通过抑制IκBα的磷酸化阻断NFκB信号通路,促进食管鳞癌细胞凋亡。
Abstract:
Aim: To evaluate the effects of capsaicin on cell apoptosis and NFκB pathway in esophageal squamous cell carcinoma (ECSS) cells. Methods:The expression levels of IκBα, phosphorylatedIκBα (pIκBα), p50 and p65 proteins in the EC9706 and Eca109 cells were deteted by Western blot. EC9706 and Eca109 cells were treated with 0,50,100,200 μmol/L capsaicin and the expressions of pIκBα and Bcl2 were detected by Western blot. The 2 cells were treated with 0,100 and 200 μmol/L capsaicin and DNAbinding activity was evaluated by electrophoretic mobility gel shift assay in EC9706 and Eca109 cells. The 2 cells were both allocated into control group,5FU group,capsaicin group ,and 5FU+capsaicin group and the apoptosis of the cells was analyzed by flow cytometry. Results: IκBα and pIκBα were expressed in the cytoplasm of ECSS cells, p50 and p65 were expressed in the nucleus of ECSS cells. With the increasing of the concentration of capsaicin, the levels of pIκBα and Bcl2 protein and the DNAbinding activity of NFκB were decreased correspondingly. Furthermore, in EC9706 and Eca109 cells, capsaicin alone increased cell apoptosis, and the effect became more prominent when it was combined with 5FU(EC9706: Fcapsaicin=2 535.497,Finteraction=113.034,P<0.001;Eca109:Fcapsaicin=5 984.040,Finteraction=5.150,P<0.05).Conclusion: Capsaicin may induce the apoptosis of ESCC cells by downregulating the activation of NFκB signaling pathway through inhibiting the phosphorylation of IκBα.

参考文献/References:

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目81071723;教育部高等学校博士点新教师科研基金资助项目20104101120002;河南省高等学校青年骨干教师 资助项目2011GGJS 010 女,1974年7月生,博士,副教授,研究方向:信号传导通路,Email:tianfang715@yahoo.com.cn
更新日期/Last Update: 2013-07-25