[1]刘伯新),郭长青)#,王曼华),等.EC9706细胞和人食管鳞状细胞癌组织中MAL基因启动子区的甲基化状态[J].郑州大学学报(医学版),2013,(04):440.
 LIU Boxin,GUO Changqing,WANG Manhua,et al.Methylation analysis of MAL gene promoter region in EC9706 cell and human esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(04):440.
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EC9706细胞和人食管鳞状细胞癌组织中MAL基因启动子区的甲基化状态
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年04期
页码:
440
栏目:
食管癌研究
出版日期:
2013-07-20

文章信息/Info

Title:
Methylation analysis of MAL gene promoter region in EC9706 cell and human esophageal squamous cell carcinoma tissue
作者:
刘伯新1郭长青1#王曼华1王明荣2
1)郑州大学第一附属医院消化内科 郑州 4500522)中国医学科学院北京协和医学院肿瘤医院肿瘤研究所分子肿瘤学国家重点实验室 北京 100021
Author(s):
LIU Boxin1)GUO Changqing1)WANG Manhua1)WANG Mingrong2)
1)Department of Gastroenterology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 2)State Key Laboratory of Molecular Oncology,Cancer Institute(Hospital),Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing 100021
关键词:
食管鳞状细胞癌EC9706细胞MAL基因启动子区甲基化
Keywords:
esophageal squamous cell carcinomaEC9706 cellMAL genepromoter region methylation
分类号:
R735.1
摘要:
目的:探讨人食管鳞状细胞癌(食管鳞癌)MAL基因启动子区的甲基化状态。 方法:利用亚硫酸氢盐测序法检测人食管鳞癌细胞系EC9706中MAL基因启动子区CpG位点甲基化的发生情况。根据测序结果选用甲基化敏感性限制性内切酶酶切结合PCR技术进一步检测26例食管鳞癌组织及配对癌旁正常组织MAL基因启动子区的甲基化情况。 结果:EC9706细胞中MAL基因启动子区检测出42个胞嘧啶位点的甲基化,占所有CpG位点的73.7%(42/57)。食管鳞癌组织中MAL基因的甲基化率为53.8%(14/26),远远高于配对癌旁正常组织的7.7%(2/26)(χ2=10.924,P<0.001)。不同临床病理特征食管鳞癌组织中MAL基因启动子区甲基化率差异均无统计学意义(P>0.05)。 结论:MAL基因启动子区的甲基化可能是食管鳞癌发生的机制之一。
Abstract:
Aim: To explore methylation status of MAL gene promoter region in human esophageal cancer. Methods:Bisulfitesequencing was used to detect the methylation pattern of MAL gene promoter region in esophageal cancer cell line EC9706.Then the methylation pattern of MAL gene promoter region in esophageal cancer and matched paracancerous tissue was further detected by methylation sensitive restriction endonuclease which was selected according to the sequencing results and PCR. Results: A total of 42 cytosine locuses were detected methylated in MAL gene promoter region in EC9706,accounting for 73.7%(42/57) of all CpG locuses. Methylation incidence of MAL gene in esophageal cancer was 53.8%(14/26),much higher than 7.7%(2/26) in matched paracancerous tissue(χ2=10.924,P<0.001). Methylation of MAL gene promoter region in esophageal cancer with different clinicopathological features had no obvious significance(P>0.05). Conclusion: The methylation of MAL gene promoter region may be one of the possible mechanisms of the occurrence of esophageal cancer.

参考文献/References:

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备注/Memo

备注/Memo:
#通讯作者,男,1966年10月生,博士,教授,主任医师,研究方向:消化道肿瘤及肝病,Email:cqguo6610@hotmail.com
更新日期/Last Update: 2013-07-25