[1]张艳),程旭芳),黄刚),等.新型核苷类似物FNC对Raji细胞增殖、凋亡和Bcl6、PRDM1、Cmyc表达的影响*[J].郑州大学学报(医学版),2013,(04):450.
 ZHANG Yan),CHENG Xufang),HUANG Gang),et al.Effect of new nucleoside analogue FNC on proliferation,apoptosis and expressions of Bcl6,PRDM1,Cmyc in cell line Raji[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(04):450.
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新型核苷类似物FNC对Raji细胞增殖、凋亡和Bcl6、PRDM1、Cmyc表达的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年04期
页码:
450
栏目:
论著
出版日期:
2013-07-20

文章信息/Info

Title:
Effect of new nucleoside analogue FNC on proliferation,apoptosis and expressions of Bcl6,PRDM1,Cmyc in cell line Raji
作者:
张艳1)程旭芳2)黄刚3)董静静3)王陈萍2)江金花1)王庆端1)#常俊标3)#
1)郑州大学医药科学研究院 郑州 4500522)郑州大学药学院 郑州4500013)郑州大学化学与分子工程学院 郑州 450001
Author(s):
ZHANG Yan1)CHENG Xufang2)HUANG Gang3) DONG Jingjing3) WANG Chenping2)JIANG Jinhua1)WANG Qingduan1)CHANG Junbiao3)
1)Academy of Medical and Pharmaceutical Sciences,Zhengzhou University, Zhengzhou 4500522)School of Pharmaceutical Sciences,Zhengzhou University,Zhengzhou 4500013)School of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001
关键词:
FNCRajiBcl6PRDM1Cmyc
Keywords:
FNCRajiBcl6PRDM1Cmyc
分类号:
R733.4
摘要:
目的:研究2脱氧2氟4叠氮核苷类似物(FNC)对Raji细胞增殖的影响及其作用机制。方法:分别用0、0.035、0.350、3.500、35.000 和 350.000 μmol/L的FNC处理 Raji 细胞24、48、72 h后,应用MTT法检测细胞增殖情况;分别用0、0.035、0.175、0.875、4.375 μmol/L FNC处理Raji细胞48 h后用流式细胞术检测细胞周期及凋亡,用RTPCR 与Western blot 法分别检测细胞中Bcl6、PRDM1、Cmyc mRNA及蛋白的表达。结果:FNC可显著抑制 Raji 细胞的增殖,呈明显的时间、剂量依赖性(F浓度=4 869.984,F时间=1 785.062,F交互=126.281, P均<0.001);FNC可诱导细胞凋亡,阻滞细胞周期于G0/G1期(P<0.05);随着FNC浓度的增加,Raji细胞中Bcl6、Cmyc mRNA及蛋白的表达降低(F=65.497、68.502和59.086、78.285,P均<0.001),PRDM1 mRNA及蛋白的表达升高(F=81.133、85.234,P均<0.001)。结论:FNC可能通过下调Bcl6、Cmyc和上调PRDM1的表达,抑制Raji 细胞的增殖,诱导细胞凋亡。
Abstract:
Aim: To investigate the effects of new nucleoside analogue 2deoxy2βfluoro4azidenucleoside analogue(FNC) on proliferation of Raji cells and its mechanism. Methods:Raji cells were treated with 0,0.035,0.350,3.500,35.000,and 350.000 μmol/L FNC for 24,48 and 72 h,then the proliferation was determined by MTT assay. Raji cells were treated with 0,0.035,0.175,0.875,4.375 μmol/L FNC for 48 h, then the cell cycle and apoptotic rate were detected by flow cytometry, and the expression levels of Bcl6,PRDM1 and Cmyc mRNA and protein were detected by RTPCR and Western blot. Results: FNC could remarkably inhibit the proliferation of Raji cells in time and dosedependent manner(Fdose=4 869.984, Ftime=1 785.062,Finteraction=126.281, P<0.001). FNC could induce cell apoptosis and arrest cell cycle in G0/G1 phase (P<0.05). The expressions of Bcl6 and Cmyc mRNA and protein decreased(F=65.497,68.502,59.086,78.285,P<0.001), while the expressions of PRDM1 mRNA and protein increased with the increasing dose of FNC(F=81.133,85.234,P<0.001). Conclusion: FNC could inhibit cell proliferation through downregulating the expressions of Bcl6 and Cmyc and upregulating the expression of PRDM1.

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备注/Memo

备注/Memo:
* 国家自然科学基金资助项目21172202;河南省科技攻关计划资助项目112101310007 #通讯作者,王庆端,男,1949年10月生,本科,研究员,研究方向:肿瘤药理,Email:wangqd@zzd.edu.cn;常俊标,男,1965年10月生,博士,教授,研究方向:药物合成,Email:changjunbiao@zzu.edu.cn
更新日期/Last Update: 2013-07-25