[1]李文杰),王明洋),白银亮),等.血清对SHSY5Y细胞吗啡戒断后ERK1/2、p38和JNK磷酸化表达的影响*[J].郑州大学学报(医学版),2013,(04):455.
 LI Wenjie,WANG Mingyang,BAI Yinliang,et al.Effects of serum on phosphorylation of ERK1/2,p38 and JNK in SHSY5Y cells during morphine withdrawal[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(04):455.
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血清对SHSY5Y细胞吗啡戒断后ERK1/2、p38和JNK磷酸化表达的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年04期
页码:
455
栏目:
论著
出版日期:
2013-07-20

文章信息/Info

Title:
Effects of serum on phosphorylation of ERK1/2,p38 and JNK in SHSY5Y cells during morphine withdrawal
作者:
李文杰1王明洋1白银亮2程月芳3李静1刘宇1张旗1#
1)郑州大学药学院临床药学系 郑州 4500012)兰州大学第二医院药剂科 兰州 7300303)河南省肿瘤医院药剂科 郑州 450008
Author(s):
LI Wenjie1) WANG Mingyang1) BAI Yinliang2) CHENG Yuefang3) LI Jing1) LIU Yu1) ZHANG Qi1)
1)Department of Clinical Pharmacology, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 4500012)Department of Pharmacy, the Second Hospital of Lanzhou University, Lanzhou 7300303)Department of Pharmacy, Henan Tumor Hospital, Zhengzhou 450008
关键词:
吗啡纳洛酮戒断血清pERK1/2pJNKpp38SHSY5Y细胞
Keywords:
morphine naloxone withdrawal serum pERK1/2pJNK pp38SHSY5Y cell
分类号:
R996
摘要:
目的:探讨胎牛血清(FBS)对人神经母细胞瘤细胞(SHSY5Y)吗啡戒断后细胞中ERK1/2、p38和JNK磷酸化表达的影响。方法:SHSY5Y细胞分为无血清培养组和体积分数为10%血清培养组(有血清培养组)。细胞经 10 μmol/L吗啡作用48 h后,10 μmol/L纳洛酮戒断。在纳洛酮戒断不同时间后,收集细胞,应用Western blot检测ERK1/2、JNK和p38蛋白磷酸化水平的变化。结果:无血清培养条件下纳洛酮戒断10 min后SHSY5Y细胞中ERK1/2磷酸化水平显著上调(F=6.619,P<0.001),戒断30、60 min后ERK1/2磷酸化水平与对照组差异无统计学意义。 有血清培养条件下纳洛酮戒断10、30和60 min后SHSY5Y细胞中ERK1/2磷酸化水平均显著上调(F=22.901,P<0.001);在无和有血清培养条件下纳洛酮戒断均引起SHSY5Y细胞中p38磷酸化水平呈时间依赖性递增,同时也能引起JNK磷酸化水平的增加,但不随戒断时间的增加而升高。结论:SHSY5Y细胞在无血清培养下,吗啡戒断时ERK1/2磷酸化为瞬时增高;血清培养下,吗啡戒断可诱导ERK1/2磷酸化持续增高。
Abstract:
Aim: To investigate the effects of fetal bovine serum (FBS) on phosphorylated ERK1/2, p38 and JNK in human neuroblastoma cells (SHSY5Y) during morphine withdrawal. Methods:SHSY5Y cells were cultured either in DMEM/F12(11) without serum or in DMEM/F12(11) with serum (10% FBS). All cells were treated with 10 μmol/L morphine for 48 h. Cells were harvested at different time after 10 μmol/L naloxone was added. Western blot was employed to detect the phosphorylation of ERK1/2, p38 and JNK. Results: ERK1/2 phosphorylation was significantly increased 10 min after naloxoneprecipitated morphine withdrawal in SHSY5Y cells cultured without FBS(F=6.619, P<0.001). However, this increase disappeared after 30 min, 60 min. On the contrary, naloxoneprecipitated morphine withdrawal induced sustained ERK1/2 phosphorylation in SHSY5Y cells cultured with 10% FBS (F=22.901, P<0.001). In contrast, naloxoneprecipitated morphine withdrawal induced similar p38 and JNK phosphorylation increase in SHSY5Y cells cultured with or without FBS. Conclusion: Morphine withdrawal induces instant increase of ERK1/2 phosphorylation when cultured without FBS while sustained increase of ERK1/2 phosphorylation in SHSY5Y cells cultured with FBS . The temporal pattern of JNK and p38 phospholyration is similar in SHSY5Y cells cultured with or without FBS.

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目30800328;河南省高校科技创新人才支持计划项目10PTGS48420 #通讯作者,男,1976年7月生,博士,教授,研究方向:生化药理与神经药理学,Email:zhangqipku@hotmail.com
更新日期/Last Update: 2013-07-25