[1]高秀艳),宋士军),崔同),等.阿魏酸对大鼠离体胸主动脉环的舒张作用及其机制*[J].郑州大学学报(医学版),2013,(04):459.
 GAO Xiuyan),SONG Shijun),CUI Tong),et al.Vasodilatation effect and its mechanism of ferulic acid on isolated thoracic aortic rings from rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(04):459.
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阿魏酸对大鼠离体胸主动脉环的舒张作用及其机制*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年04期
页码:
459
栏目:
论著
出版日期:
2013-07-20

文章信息/Info

Title:
Vasodilatation effect and its mechanism of ferulic acid on isolated thoracic aortic rings from rats
作者:
高秀艳1宋士军2崔同3王腾腾1李朝苹1田粟1#
1)河北医科大学公共卫生学院营养与食品卫生学教研室 石家庄 0500172)河北医科大学基础医学院生理学教研室 石家庄 0500173)河北农业大学食品科技学院 保定 071001
Author(s):
GAO Xiuyan1) SONG Shijun2) CUI Tong3) WANG Tengteng1) LI Zhaoping1) TIAN Su1)
1)Department of Nutrition and Food Hygiene, School of Public Health, Hebei Medical University, Shijiazhuang 0500172)Department of Physiology,School of Basic Medical Sciences, Hebei Medical University, Shijiazhuang 0500173)College of Food Science & Technology, Agricultural University of Hebei, Baoding 071001
关键词:
阿魏酸胸主动脉环舒张作用机制大鼠
Keywords:
ferulic acid thoracic aortic ring vasodilatation effect mechanismrat
分类号:
R151.1
摘要:
目的:探讨阿魏酸(FA)对大鼠离体胸主动脉环的舒张作用。方法:采用离体血管灌流法,检测FA对去氧肾上腺素(PE)和KCl预收缩大鼠离体胸主动脉环的舒张作用,观察去内皮或L亚硝基精氨酸甲基酯(LNAME)、亚甲蓝(MB)、吲哚美辛(Indo)、格列苯脲(Gli)、四乙基胺(TEA)孵育后舒张作用的变化;并观察在无钙KH液中FA对PE诱发动脉环收缩作用的影响。结果:FA具有浓度依赖性地舒张PE和KCl预收缩的胸主动环的作用;完整内皮组和去内皮组的舒张作用的pD2分别为2.62±0.08和2.60±0.09(Z=0.447,P=0.655),与各种受体阻断剂孵育后FA的舒张作用间差异无统计学意义(P>0.05)。FA可明显抑制PE诱发的依外Ca2+性收缩反应(FFA=134.517,P<0.001)和依内Ca2+性收缩反应(F=108.816,P<0.001)。结论:FA具有舒张动脉环作用,其机制与抑制平滑肌细胞内Ca2+的释放和外Ca2+内流有关,其中考虑以抑制细胞内Ca2+的释放为主。
Abstract:
Aim: To investigate the vasodilatation effects and mechanism of ferulic acid (FA) on isolated thoracic aortic rings from rats. Methods: Using isolated vascular methods, the vasodilatation effects of accumulated FA on thoracic aortic rings preconstricted with Lphenylephrine hydrochloride (PE) and KCl were observed. After the isolated thoracic aortic rings were pretreated with removing the endothelium, or pretreated with the drugs, the changes of vasodilatation effects were observed. And in Ca2+free KH solution, the influence of FA on thoracic aortic rings proconstricted with PE was observed. Results: With the increasing concentration, FA increased the vasodilatation effects of vascular aortic ring in a dosedependent manner. The values of pD2 of endothelial groups and the endothelialdenuded group were 2.62±0.08, 2.60±0.93, respectively (Z=0.447,P=0.655). Compared with them, there was no significant changes after incubation with the drugs of LNAME, Indo, Gli, TEA, or MB (P>0.05). In addition, FA inhibited PEinduced contraction in Ca2+free KH solution(FFA=134.517,P<0.001). In Ca2+depleted, PEtreated aortic rings, FA inhibited the Ca2+induced contraction in a concentrationdependent manner(F=108.816,P<0.001). Conclusion: These results indicate that FA relaxes the thoracic aortic rings of rats in an endotheliumindepedended manner, and the vasorelaxing effect of FA might be mediated mainly through inhibiting Ca2+ release from intracellular stores and extracellular Ca2+ influx into vascular smooth muscle cells.

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备注/Memo

备注/Memo:
*河北省自然科学基金资助项目C2011204093;中国博士后科学基金资助项目20070410870 #通讯作者,女,1971年11月生,博士,副教授,研究方向:食品功效成分解析,Email: sutian314@hebmu.edu.cn
更新日期/Last Update: 2013-07-25