[1]刘长海)△,华春秀),归改霞),等.和厚朴酚对小鼠四氯化碳肝损伤的保护作用*[J].郑州大学学报(医学版),2013,(04):470.
 LIU Changhai),HUA Chunxiu),GUI Gaixia),et al.Protective effect of honokiol on liver injury induced by carbon tetrachloride in mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(04):470.
点击复制

和厚朴酚对小鼠四氯化碳肝损伤的保护作用*
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年04期
页码:
470
栏目:
论著
出版日期:
2013-07-20

文章信息/Info

Title:
Protective effect of honokiol on liver injury induced by carbon tetrachloride in mice
作者:
刘长海1)华春秀2)归改霞2)刘锦辉2)李贺文2)夏西超2)#
1)南阳医学高等专科学校第一附属医院检验科 南阳 4730582)南阳医学高等专科学校基础医学院 南阳 473061
Author(s):
LIU Changhai1) HUA Chunxiu2) GUI Gaixia2) LIU Jinhui2) LI Hewen2)XIA Xichao2)
1)Clinical Laboratory, the First Affiliated Hospital, Nanyang Medical College, Nanyang 473058 2)Department of Basic Medical, Nanyang Medical College, Nanyang 473061
关键词:
和厚朴酚小鼠肝损伤四氯化碳
Keywords:
honokiolmouseliver injurycarbon tetrachloride
分类号:
R575
摘要:
目的:探讨和厚朴酚(HNK)对四氯化碳(CCl4)诱导的肝损伤的保护作用。方法:将50只小鼠随机分成HNK高、中、低剂量组(分别以20.0、2.0、0.2 mg/kg剂量皮下注射HNK注射液),正常组和模型组(注射等量生理盐水)5组。给药7 d后,模型组和HNK各剂量组腹腔注射含CCl4的大豆油溶液,正常组注射等体积大豆油溶液。24 h后,摘除眼球取血,解剖肝脏,观察小鼠肝脏的病理学变化,应用生化分析仪测定血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、血糖(Glu)含量,Realtime PCR法分析血清中ALT、AST、ALP和肝脏中肿瘤坏死因子α(TNFα)、白介素6(IL6)、干扰素γ(IFNγ)mRNA的表达。结果:HE染色结果显示,正常小鼠肝组织结构完整,肝细胞呈索状结构排列,肝细胞分界清晰,未见脂肪变性和炎性细胞浸润。模型组小鼠肝组织肝索排列紊乱,中央静脉变形,肝细胞肿胀并呈泡状脂肪变性,伴淋巴细胞和中性粒细胞浸润。HNK各剂量组上述病理变化均有不同程度减轻,尤以HNK高剂量组改善较明显。与模型组相比,HNK处理后,小鼠血清中ALT、AST、ALP含量和mRNA水平及Glu浓度均明显降低(F分别为274.620,1 121.720;355.870,69.430;294.280,35.330;75.770;P均<0.001);同时,肝脏组织中TNFα、IL6 和IFNγ mRNA的表达明显降低(F分别为1 165.950,69.460,4 787.190;P均<0.001)。结论:HNK对CCl4诱导的小
Abstract:
Aim: To observe the protective effect of honokiol on liver injury induced by carbon tetrachloride (CCl4) in mice.Methods:Fifty mice were randomly divided into five groups.The HNK high, middle and low dose groups were injected honokiol solution at a concentration of 20.0, 2.0, and 0.2 mg/kg, respectively, and the normal group and model group were injected normal saline, respectively. After 7 d,animals in the model group and HNK groups were injected 10 mL/kg CCl4, others were given soybean oil. Histological analysis of liver was performed by hematoxylin and eosin. The contents of serum alanine aminotransferase(ALT), aspartate aminotransferase(AST), alkaline phosphatase(ALP) and glucose(Glu) were determined through automatic biochemistry analyzer. The mRNA levels of ALT, AST, ALP, tumor necrosis factorα(TNFα), interleukin6(IL6) and interferonγ(INFγ) in livers were analyzed by realtime PCR.Results: Compared with the normal group, the liver sections of model group showed destructive alterations in the parenchyma, extensive fatty changes, disarrangement of hepatic cords and central veins, centrilobular necrosis, focal necrosis.The content of ALT, AST, ALP,and Glu in HNK groups were lessened in comparison with control group (F=274.620,1 121.720;355.870,69.430;294.280,35.330;75.770;P<0.001). The mRNA expression of TNFα, IL6 and IFNγ in liver was decreased in comparison with control group(F=1 165.950,69.460,4 787.190;P<0.001)significantly.Conclusion: The HKK can play a protecting role against liver injury induced by CCl4.

参考文献/References:

[1]薛芳, 李世辉, 潘崚. 和厚朴酚对U937细胞的体外作用 [J]. 中成药, 2007, 29(11):1582
[2]Ho KY, Tsai CC, Chen CP, et al. Antimicrobial activity of laonokiol and magnolol isolated from Magnolia officinalis [J]. Phytother Res, 2001, 15(2):139
[3]Bai X, Cerimele F, UshioFukai M, et al. Honokiol, a small molecular weight natural product, inhibits angiogenesis in vitro and tumor growth in vivo [J].J Biol Chem, 2003, 278(37):35501
[4]Zheng J, Tang Y, Sun M, et al. Characterization, pharmacokinetics, tissue distribution and antitumor activity of honokiol submicron lipid emulsions in tumorburdened mice [J]. Pharmazie, 2013,68(1):41
[5]Tian W, Xu D, Han W, et al. Cyclophilin D modulates cell death transition from early apoptosis to programmed necrosis induced by honokiol [J]. Int J Oncol, 2013, 42(5): 1654
[6]Chae JI, Jeon YJ, Shim JH, et al. Downregulation of Sp1 is involved in honokiolinduced cell cycle arrest and apoptosis in human malignant pleural mesothelioma cells [J]. Oncol Rep, 2013, 29(6):2318
[7]Kumar A, Kumar Singh U, Chaudhary A. Honokiol analogs: a novel class of anticancer agents targeting cell signaling pathways and other bioactivities [J]. Future Med Chem, 2013,5(7):809
[8]陈伟,林冠文,张青.和厚朴酚诱导人非霍奇金淋巴瘤Raji细胞凋亡及其可能机制 [J]. 南方医科大学学报,2011,31(11):1918
[9]王向军. 五味草水提物与醇提物预处理对四氯化碳致小鼠急性肝损伤的保护作用 [J]. 郑州大学学报:医学版, 2012,47(1):49
[10]刘伟萍, 金国平, 陈培波, 等. 山药水提物对四氯化碳所致小鼠急性肝损伤的改善作用 [J]. 郑州大学学报:医学版,2008,43(5):885
[11]彭福江,屈丹丹,郭亚兵. 臭氧化盐水对四氯化碳诱导的大鼠急性肝损伤的保护作用 [J]. 南方医科大学学报,2010,30(3):633
[12]林英,朱曦,么改琦, 等. 重组人生长激素对肠源性内毒素血症大鼠急性肝损伤的影响 [J]. 解放军医学杂志,2011,36(2):158
[13]Adetoro KO, Bolanle JD, Abdullahi SB, et al. In vivo antioxidant effect of aqueous root bark, stem bark and leaves extracts of Vitex doniana in CCl4 induced liver damage rats [J]. Asian Pac J Trop Biomed, 2013, 3(5):395
[14]Domitrovic R, Jakovac H. Effects of standardized bilberry fruit extract (Mirtoselect) on resolution of CCl4induced liver fibrosis in mice [J]. Food Chem Toxicol, 2011, 49(4): 848
[15]Yang L, Wang CZ, Ye JZ, et al.Hepatoprotective effects of polyprenols from Ginkgo biloba L. leaves on CCl4induced hepatotoxicity in rats [J]. Fitoterapia, 2011, 82(6):834
[16]Diao Y, Zhao XF, Lin JS, et al. Protection of the liver against CCl4induced injury by intramuscular electrotransfer of a kallistatinencoding plasmid [J]. World J Gastroenterol, 2011, 17(1): 111
[17]Yoshioka K, Kunitomo M, Yanai K, et al. Hepatocyte nuclear factor 1β induced by chemical stress accelerates cell proliferation and increases genomic instability in mouse liver [J]. J Recept Signal Transduct Res, 2011, 31(2): 132
[18]金黎明,杨艳,刘万顺, 等. 氨基葡萄糖对CCl4所致小鼠肝损伤的改善作用 [J]. 郑州大学学报:医学版, 2009,44(1): 122

相似文献/References:

[1]梁璐△,陈小军,吴昊,等.人脐带间充质干细胞在小鼠体内的迁移变化及宿主的免疫反应*[J].郑州大学学报(医学版),2010,(01):33.
 LIANG Lu,CHEN Xiaojun,WU Hao,et al.Survival and homing of human umbilical cord mesenchymal stem cells in immunocompetent mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(04):33.
[2]许燕),高国伟),鄢文海),等.低速离心法分离小鼠胚胎干细胞*[J].郑州大学学报(医学版),2010,(01):75.
 XU Yan),GAO Guowei),YAN Wenhai),et al.A new method of mouse embryonic stem cells isolation[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(04):75.
[3]郭艺红,孙莹璞#,乔玉环,等.不同用药方案对小鼠种植窗期子宫内膜白血病抑制因子及胞饮突表达的影响*[J].郑州大学学报(医学版),2010,(02):202.
 GUO Yihong,SUN Yingpu,QIAO Yuhuan,et al.Expression of LIF and pinopodes in mouse endometrium during the implantation window based on different treatment protocols[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(04):202.
[4]符粤文))△,吴德沛),陈峰),等.单倍型骨髓移植小鼠移植物抗宿主病模型的建立及移植物抗宿主病靶器官T细胞受体克隆检测*[J].郑州大学学报(医学版),2009,(06):1171.
 FU Yuewen)),WU Depei),CHEN Feng),et al.Establishment of murine haploidentical bone marrow transplantation model and detection of T cell receptor clonal in target organs of graftversushost disease[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):1171.
[5]唐蓓△.小鼠Arl8a基因逆转录病毒载体MSCVGFPArl8a的构建*[J].郑州大学学报(医学版),2009,(06):1204.
 TANG Bei.Cloning of mouse Arl8a gene and construction of its retroviral vector[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):1204.
[6]杨雯雯),常树丽),李艳菲),等.氯化汞对小鼠睾丸间质瘤mLTC1细胞增殖和孕酮合成的影响[J].郑州大学学报(医学版),2009,(06):1253.
 YANG Wenwen),CHANG Shuli),LI Yanfei),et al.Effects of mercury chloride on cell growth and progesterone biosynthesis of mLTC1 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):1253.
[7]王红伟)△,范玲玲),任爱红),等.氯胺酮对小鼠离体十二指肠纵行肌条收缩的影响*[J].郑州大学学报(医学版),2009,(05):990.
 WANG Hongwei),FAN Lingling),REN Aihong),et al.Effect of ketamine on contraction of isolated duodenal longitudinal strips in mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):990.
[8]张菊娥)△,陈小让),张翠英),等.四君子汤加柴胡对长期摄酒小鼠血脂及抗氧化作用的影响[J].郑州大学学报(医学版),2009,(05):1045.
 ZHANG Jue),CHEN Xiaorang),ZHANG Cuiying),et al.Effect of Sijun decoction with bupleurum falcatum on blood lipid and its antioxidation ability in alcoholintake mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):1045.
[9]朱宝菊),乔玉环)#,李印).canstatin基因治疗对小鼠人卵巢癌移植瘤生长及Caspase3、Flk1蛋白表达的影响*[J].郑州大学学报(医学版),2009,(02):292.
 ZHU Baoju),QIAO Yuhuan),LI Yin).Effects of canstatin gene theraphy on expression of Flk1 and Caspase3 and growth of human ovarian cancer in mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):292.
[10]刘青),刘剑波)#.干扰素γ对哮喘小鼠肺组织中水通道蛋白5表达的影响*[J].郑州大学学报(医学版),2009,(02):306.
 LIU Qing),LIU Jianbo).Effects of interferongamma on expression of aquaporin5 in lung tissue of asthmatic mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):306.

备注/Memo

备注/Memo:
*河南省重点科技攻关基金资助项目13B310177 #通讯作者,男,1977年4月生,博士,副教授,研究方向:药理学,Email:xiaxichao8336@163.com
更新日期/Last Update: 2013-07-25