[1]冯青青,夏熙郑#.慢性阻塞性肺疾病患者肺组织中Bach1和γGCS的表达[J].郑州大学学报(医学版),2013,(04):474.
 FENG Qingqing,XIA Xizheng.Expressions of Bach1 and γGCS in lung tissue of patients with chronic obstructive pulmonary disease[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(04):474.
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慢性阻塞性肺疾病患者肺组织中Bach1和γGCS的表达
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年04期
页码:
474
栏目:
论著
出版日期:
2013-07-20

文章信息/Info

Title:
Expressions of Bach1 and γGCS in lung tissue of patients with chronic obstructive pulmonary disease
作者:
冯青青夏熙郑#
郑州大学第二附属医院呼吸内科 郑州 450014
Author(s):
FENG Qingqing XIA Xizheng
Department of Respiratory, the Second Affiliated Hospital,Zhengzhou University, Zhengzhou 450014
关键词:
慢性阻塞性肺疾病BTBCNC异体同源体1γ谷氨酰半胱氨酸合成酶
Keywords:
obstructive pulmonary disease BTB and CNC homology 1γglutamylcysteine synthetase
分类号:
R563.3
摘要:
目的:研究慢性阻塞性肺疾病(COPD)患者肺组织中BTBCNC异体同源体1(Bach1)和γ谷氨酰半胱氨酸合成酶(γGCS)的表达。方法:收集因肺肿瘤行肺叶切除术患者的肺组织标本,诊断符合COPD者入选COPD组(20例),不符合者入选对照组(20例)。于远离病灶处取材,分别采用原位杂交和免疫组化方法检测肺组织中Bach1、γGCS mRNA和蛋白的表达。结果:COPD组患者肺组织中Bach1、γGCS mRNA和蛋白的表达均较对照组增强(t分别为9.946,14.573, 14.134,14.365,P均<0.001)。COPD患者肺组织中Bach1 蛋白与γGCS mRNA、蛋白表达均呈直线负相关(r为-0.834, -0.815,P<0.001)。结论:Bach1可能通过负调控γGCS基因的表达,从而在COPD的氧化/抗氧化调节过程中发挥重要作用。
Abstract:
Aim: To investigate the expressions of BTB and CNC homology 1(Bach1)and γglutamylcysteine synthetase(γGCS) in lung tissue of the patients with chronic obstructive pulmonary disease(COPD).Methods:Lung specimens were obtained from 40 patients who underwent lobectomy for lung tumor, of whom 20 had concurrent COPD and 20 without COPD.The samples were taken from the areas remote to the lesion, and in situ hybridization and immunohistochemistry were used to detect the mRNA and protein expressions of Bach1 and γGCS,respectively.Results: The mRNA and protein expression levels of Bach1 and γGCS in COPD group were significantly higher than those in the control group(t was 9.946,14.573,14.134,14.365,respectively,P<0.001). The Bach1 protein expression level was negatively correlated with those of γGCS mRNA and protein (r=-0.834, -0.815, P<0.001) in COPD group. Conclusion: Bach1 may negatively regulate the expression of γGCS gene,and play an important role in the process of oxidation/antioxidation of COPD.

参考文献/References:

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备注/Memo

备注/Memo:
#通讯作者,男,1955年12月生,本科,教授,研究方向:慢性阻塞性肺疾病和呼吸系统感染性疾病,Email:xiaxizheng@126.com
更新日期/Last Update: 2013-07-25